In vitro-cultivation of human periosteum derived cells in bioresorbable polymer-TCP-composites

Arnold, Ulrike

doi:10.1016/s0142-9612(01)00364-7

Cited by 56 publications

(52 citation statements)

References 40 publications

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“…Recently, Endres et al (2007) showed that the 3D arrangement of human articular chondrocytes in human fibrin glue and resorbable polyglycolic acid (PGA) scaffolds, cultured in the presence of human serum, is an excellent system for the maturation of cartilage grafts in articular cartilage regeneration. Several previous studies also demonstrated that the fibrin immobilisation techniques promoted homogeneous cell distribution and bone formation, when using human periosteum-derived progenitor cells in PLGA (Zheng et al, 2006), PLGA-TCP composites (Arnold et al, 2002) and PLGApolydioxanon fleeces (Perka et al, 2000).…”

Section: Introductionmentioning

confidence: 90%

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

Munirah¹,

Sh²,

Ruszymah³

et al. 2008

eCM

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Our preliminary results indicated that fibrin and poly(lacticco-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl 2 ) solution. PLGA-seeded chondrocytes were used as a control. Resulting constructs were implanted subcutaneously, at the dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and sulphated-glycosaminoglycan (sGAG) analyses were performed at each time point of 1, 2 and 4 weeks postimplantation. Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and glycosaminoglycan (GAG) in fibrin/PLGA hybrid constructs was confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene encoded cartilage-specific markers, collagen type II and aggrecan core protein. The sGAG production in fibrin/PLGA hybrid constructs was higher than in the PLGA group. In conclusion, fibrin/PLGA hybrid scaffold promotes cartilaginous tissue formation in vivo and may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering.

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Section: Introductionmentioning

confidence: 90%

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

Munirah¹,

Sh²,

Ruszymah³

et al. 2008

eCM

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“…Secondly, their osteogenic potential persists even after prolonged cultivation. Longer culture periods (3-4 months) can be performed before final differentiation is obtained, making these cultures suitable for long-term in vitro evaluation of biomaterials (Perka et al 2000;Arnold et al 2002). A point to note here is that selection of the appropriate cultures having an osteogenic potential is a necessity.…”

Section: Discussionmentioning

confidence: 99%

“…The latter renders these cells less suitable for long-term in vitro test systems. The use of progenitor cells (periosteum or bone marrow derived cells) may circumvent these problems (Ishaug et al 1997;Perka et al 2000;Arnold et al 2002;Spitzer et al 2002;Lisignoli et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, several attempts to culture and differentiate periosteum-derived cells towards the osteogenic lineage were made (Koshihara et al 1987(Koshihara et al , 1989Breitbart et al 1998;Solchaga et al 1998). Some studies to evaluate the osteogenic potential of biomaterials were performed which used periosteum-derived cells from human, rabbit, pig and bovine origin (Perka et al 2000;Arnold et al 2002;Spitzer et al 2002). However, rat periosteum-derived cells were not yet analyzed with regard their suitability to study cell/biomaterial interactions.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Osteogenic Differentiation of Rat Periosteum-derived Cells

2005

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Selection of appropriate cultures having an osteogenic potential is a necessity if cell/biomaterial interactions are studied in long-term cultures. Osteoblastic cells derived from rat long bones or calvaria have the disadvantage of being in an advanced differentiation stage which results in terminal differentiation within 21 days. In this regard, less differentiated periosteum-derived osteoprogenitors could be more suitable. Periosteum-derived cells were isolated from the tibiae of adult Wistar rats (n = 12). The osteogenic potential with regard to alkaline phosphatase activity, morphology, nodule formation and mineralization was studied by culturing them in an osteogenic medium for up to 4 months. Seventy-five percent of the cultures (n = 9) did not show any increase in alkaline phosphatase activity nor nodule formation during long-term culture for up to 4 months. Nevertheless, in 25% of the cultures, alkaline phosphatase activity started from negligible (<5 mM pNP/mg protein) and increased towards approximately 50 mM pNP/mg protein. Three-dimensional nodule formation was observed at passages 3-5. In further passages (P5-P7), nodule formation capacity decreased and a diffuse mineralization pattern was observed. Suitable cultures with osteogenic capacity, can be selected at early passages based on the presence of cuboidal cells. These cells have the advantage of retaining their osteogenic potential even after prolonged cultivation (6-7 passages) before final differentiation occurs. Although periosteal cells are suitable for long term in vitro evaluation of biomaterials, the isolation and selection is time consuming. Hence, a more appropriate source to study cell/biomaterial interactions should be more convenient.

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“…Both these calcium phosphates offer different degrees of osteoclastic resorbability and solubility 14) . TCP has also proven to be of good biocompatibility and biodegradability and is highly osteoconductive 15) . Healing of bone defects requires cell proliferation and new matrix production to bridge the damaged tissue.…”

Section: Introductionmentioning

confidence: 99%

Mechanical and biological effects of infiltration with biopolymers on 3D printed tricalciumphosphate scaffolds

Cornelsen

Probst²,

Schwarz³

et al. 2017

Dent. Mater. J.

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The aim of this study was to evaluate the influence of infiltrating 3D printed (TCP) scaffolds with different biodegradable polymers on their mechanical and biological properties. 3D printed TCP scaffolds with interconnecting channels measuring 450±50 µm were infiltrated with four different biodegradable copolymers. To determine the average compressive strength, a uniaxial testing system was used. Additionally, scaffolds were seeded with MC3T3 cells and cell viability was assessed by live/dead-assay. Uninfiltrated TCP had an average compression strength of 1.92±0.38 MPa. Mechanical stability was considerably increased in all infiltrated scaffolds up to a maximum of 7.36±0.57 MPa. All scaffolds demonstrated high cell survival rates with a maximum of 94±10 % living cells. In conclusion, infiltration of 3D printed tricalcium phosphate scaffolds with biodegradable polymers significantly improved mechanical properties and biological properties were comparable to those of uninfiltrated TCP scaffolds.

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In vitro-cultivation of human periosteum derived cells in bioresorbable polymer-TCP-composites

Cited by 56 publications

References 40 publications

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

Isolation and Osteogenic Differentiation of Rat Periosteum-derived Cells

Mechanical and biological effects of infiltration with biopolymers on 3D printed tricalciumphosphate scaffolds

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