Mechanical and biological effects of infiltration with biopolymers on 3D printed tricalciumphosphate scaffolds

Cornelsen, Matthias; Probst, Florian; Schwarz, Christina; Burian, Egon; Tröltzsch, Matthias; Otto, Sven; Saller, Maximilian Michael; Schieker, Matthias; Seitz, Hermann

doi:10.4012/dmj.2016-306

Cited by 6 publications

(9 citation statements)

References 38 publications

(47 reference statements)

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“…The scaffold used in our study is a synthetic biodegradable composite or hybrid, based on the ceramic tri-calcium phosphate (TCP) that is infiltrated with the polymer poly(D,L-lactide-co-glycolide (PLGA), abbreviated as TCP-PLGA. The infiltration of polymers such as PLGA significantly improves the mechanical properties in comparison to non-infiltrated TCP scaffolds, therefore balancing the problem of brittleness [20][21][22] . PLGA was chosen among other polymers because in a previous work of our study group it demonstrated favourable mechanical and biological behaviour 20 .…”

Section: Discussionmentioning

confidence: 99%

“…The infiltration of polymers such as PLGA significantly improves the mechanical properties in comparison to non-infiltrated TCP scaffolds, therefore balancing the problem of brittleness [20][21][22] . PLGA was chosen among other polymers because in a previous work of our study group it demonstrated favourable mechanical and biological behaviour 20 . The scaffolds were produced by 3D printing based on principles of CAD/CAM.…”

Section: Discussionmentioning

confidence: 99%

“…Although all TCP-PLGA scaffolds in this study were applicable to the defect area and could be fixed well via titanium screws, a resorbable osteosynthesis system based on biodegradable polymers such as PDLLA or PLGA may allow for a better connection to the TCP-PLGA construct 62 . Even though the mechanical properties of TCP are improved by the infiltration of a polymer, these scaffolds still have the drawback of brittleness and further improvements are desirable 20 .…”

Section: Discussionmentioning

confidence: 99%

“…www.nature.com/scientificreports www.nature.com/scientificreports/ CAD/CAM-fabrication of scaffolds. All 3D in-vitro experiments were carried out with TCP-PLGA cylinders of 20 mm height and 10 mm diameter (volume ~1.57 cm 3 ) with interconnecting channels of 450-500 μm as previously described 20 . TCP-PLGA blocks were used in the in-vivo experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The STL data were transferred to the Chair of Fluid Technology and Microfluidics at the University of Rostock where customized TCP-PLGA (tri-calcium phosphate infiltrated with poly(D,L-lactide-co-glycolide)) composite scaffold were finally manufactured by CAD/CAM. Further scaffolds specifications were previously described 20 .…”

Section: Methodsmentioning

confidence: 99%

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Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst

Fliefel

Burian

et al. 2020

Sci Rep

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Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADScs) with tri-calcium phosphates (tcp) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixteen skeletally mature miniature pigs were divided into two groups: Control group and scaffolds seeded with osteogenic differentiated pADSCs (n = 8/group). TCP-PLGA scaffolds with or without cells were integrated in the mandibular critical size defects and fixed by titanium osteosynthesis plates. After 12 weeks, ADSCs seeded scaffolds (n = 7) demonstrated significantly higher bone volume (34.8% ± 4.80%) than scaffolds implanted without cells (n = 6, 22.4% ± 9.85%) in the micro-CT (p < 0.05). Moreover, an increased amount of osteocalcin deposition was found in the test group in comparison to the control group (27.98 ± 2.81% vs 17.10 ± 3.57%, p < 0.001). In conclusion, ADSCs seeding on ceramic/polymer scaffolds improves bone regeneration in large mandibular defects. However, further improvement with regard to the osteogenic capacity is necessary to transfer this concept into clinical use. Maxillofacial bone defects, which occur due to trauma, craniofacial deformities, tumour or infection can lead to facial deformities and severe maxillofacial dysfunctions provoking a dramatic decrease in the quality of life of the patients 1,2. The reconstruction of large bone defects poses many challenges in oral and maxillofacial surgery. Although autologous bone grafts are considered the gold standard in bone defect repair, there are some concerns related to the limited supply and donor site morbidity 3. Several alternatives as allografts, xenografts or synthetic bone substitutes have been brought by researchers and clinicians to restore the function and architecture of the defective bone but still cannot solve the problem due to various limitations. The search of new treatment alternatives has emerged enormously in the past few

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst

Fliefel

Burian

et al. 2020

Sci Rep

Self Cite

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A Review on Materials Application in Scaffold Design by Fused Deposition Method

Ansari,

Sheikh

2023

J. Inst. Eng. India Ser. C

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Effect of infiltrant-related parameters on mechanical performance for 3DP technology

Wang

Ding

et al. 2020

RPJ

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Purpose The effects of infiltrant-related factors during post-processing on mechanical performance are fully considered for three-dimensional printing (3DP) technology. The factors contain infiltrant type, infiltrating means, infiltrating frequency and time interval of infiltrating. Design/methodology/approach A series of printing experiments are conducted and the parts are processed with different conditions by considering the above mentioned four parameters. Then the mechanical performances of the parts are tested from both macroscopic and microscopic papers. In the macroscopic view, the compressive strength of each printed part is measured by the materials testing machine – Instron 3367. In the microscopic view, scanning electron microscope and energy dispersion spectrum are used to obtain microstructure images and element content results. The pore size distributions of the parts are measured further to illustrate that if the particles are bound tightly by infiltrant. Then, partial least square (PLS) is used to conduct the analysis of the influencing factors, which can solve the small-sample problem well. The regression analysis and the influencing degree of each factor are explored further. Findings The experimental results show that commercial infiltrant has an outstanding performance than other super glues. The infiltrating action will own higher compressive strength than the brushing action. The higher infiltrating frequency and inconsistent infiltrating time interval will contribute to better mechanical performance. The PLS analysis shows that the most important factor is the infiltrating method. When compare the fitted value with the actual value, it is clear that when the compressive strength is higher, the fitting error will be smaller. Practical implications The research will have extensive applicability and practical significance for powder-based additive manufacturing. Originality/value The impact of the infiltrating-related post-processing on the performance of 3DP technology is easy to be ignored, which is fully taken into consideration in this paper. Both macroscopic and microscopic methods are conducted to explore, which can better explain the mechanical performance of the parts. Furthermore, as a small-sample method, PLS is used for influencing factors analysis. The variable importance in the projection index can explain the influencing degree of each parameter.

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Mechanical and biological effects of infiltration with biopolymers on 3D printed tricalciumphosphate scaffolds

Cited by 6 publications

References 38 publications

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

A Review on Materials Application in Scaffold Design by Fused Deposition Method

Effect of infiltrant-related parameters on mechanical performance for 3DP technology

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