In vitro culture and diversity of endophytic fungi in Bambusa oldhamii

Pasqualini, Ana Paula de Azevedo; Santos, Mariely Cristine dos; ́Anna-Santos, Bruno Francisco Sant; Fraga, Hugo Pacheco de Freitas; Quoirin, Marguerite

doi:10.1590/1983-40632019v4953760

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…Medium preparation: Standard MS medium (24) provided by Caisson company was used, then 30 g L -1 of sucrose was added and then the necessary growth regulators were added according to the experiment requirements. Add 1 mL of PPM (Plant Preservative Mixture) to reduce fungal and bacterial contamination (28). Also, add activated charcoal at a concentration of 4.0 g L -1 and adjust the pH to 5.7-5.8 with NaOH or HCl, then add 7.0 g L -1 agar to the medium.…”

Section: Methodsmentioning

confidence: 99%

ROLE OF DIMETHYL SULFATE ON BIOCHEMICAL CHARACTERISTICS OF Fragaria ananassa Duch UNDER SALINITY STRESS IN VITRO

Yasir¹,

Hamad²,

Neamah³

2022

Iraqi J. Agric. Sci.

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The aims of this study were to evaluate the effect of four concentrations of Dimethylsulfate (DMS) 0%, 0.03%, 0.06% and 0.1% under four levels of salinity including 0, 30, 60 and 90 mmol NaCl L-1 on some biochemistry characters such as proline, hydrogen peroxide (H2O2), catalase (CAT), superoxide dismutase (SOD), chlorophyll a (Chl a), chlorophyll b (Chl b) and total chlorophyll. All treatments were distributed according to the factorial experiment using complete ro(CRD) with five replicates. Results revealed that 0.03% of DMS gave the highest proline of 2.08 mg g-1, H2O2 of 0.64 µM 100mg-1, catalase of 19.53 AU min-1g-1 and SOD of 116.8 IU 100mg-1. The control achieved the highest Chl a of35.75 mg g-1, Chl b of 23.38 mg g-1 and total chlorophyll of 79.00 mg g-1 . Furthermore, 30 mM NaCl L-1 possesses highest proline of 2.14 mg g-1, H2O2 of 0.62 µM 100mg-1, catalase of 19.38 AU min-1g-1 and SOD of 114.0IU 100mg-1. The non-saline plantlets (control) gave highest Chl.a of 33.00 mg g-1, Chl b 20.62 mg g-1. While 60 mmol NaCl L-1 gave biggest total chlorophyll of 74.75.

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Section: Methodsmentioning

confidence: 99%

ROLE OF DIMETHYL SULFATE ON BIOCHEMICAL CHARACTERISTICS OF Fragaria ananassa Duch UNDER SALINITY STRESS IN VITRO

Yasir¹,

Hamad²,

Neamah³

2022

Iraqi J. Agric. Sci.

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“…To achieve this aim, it is necessary to establish disinfected explants in vitro for subsequent successful micropropagation of this specie. However, the difficulty of disinfecting Persian lime explants during the in vitro establishment phase is limited, due to the high rate of microbial, fungal, and necrotic contamination (phenol exudate products) [20]. Therefore, it is necessary to use biocides, fungicides, antibiotics, whose functions have been described as alternatives to control the growth of fungi and bacteria endophytes during in vitro establishment [21].…”

Section: Discussionmentioning

confidence: 99%

In vitro establishment from mature nodal segments of Persian Lime (Citrus latifolia Tan.)

Humberto Estrella-Maldonado,

Julio Ventura-Bello,

Ricardo Santillán-Mendoza

et al. 2024

Int. J. Sci. Res. Arch.

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In vitro propagation is a technique that has been used to produce large-scale, healthy, pathogen-free plants. However, the presence of endophytic microorganisms in explant tissues is one of the limiting factors for the in vitro establishment of any species. In this sense, we studied the effectiveness of PPM® biocide (Plant Preservative Mixture) and Chlorothalonil fungicide to control and/or reduce microbial and fungal contamination in mature nodal segments of different Persian lime clones under in vitro conditions. The results showed that explants cultured in MS medium (Murashige and Skoog 1962) supplemented with 0.2 mL L-1 of PPM biocide + 1.0 mL L-1 of Chlorothalonil were adequate to achieve a higher percentage of disinfection (44%) in mature nodal segments in the Persian lime clones. Furthermore, it was observed that PPM concentrations above 0.3 mL L-1 resulted in greater phytotoxicity in mature nodal segments at 60 days.

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“…Isolates 711 and 712 were identified to the genus Phoma, a diverse genus that includes, in addition to growthpromoting microorganisms (Hamayun et al 2009), phytopathogens, entomopathogens, soil saprophytes, aquatic saprophytes and opportunistic human pathogens (Rai et al 2009). Endophytic fungi of B. oldhamii were previously reported by Pasqualini et al (2019), which report that research associated with the discovery of these isolates may increase the current knowledge about the diversity of fungi associated with bamboo. They report the isolation of phylogenetically similar isolates to those found in the present paper, which include: Phoma herbarum, Arthrinium kogelbergense, Arthrinium arundinis, Fusarium graminearum, Wojnowiciella leptocarpi, and Fusarium sp.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and characterization of endophytic fungi of Bambusa oldhamii munro applied as antagonists to Pyricularia oryzae

et al. 2020

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Isolation, identification and characterization of endophytic fungi of Bambusa oldhamii munro applied as antagonists to Pyricularia oryzae 1 Endophytic fungi, in addition to improving plant development, may produce antimicrobial substances that can inhibit pathogens. The objective of this study was to isolate and characterize endophytic fungi of Bambusa oldhamii and evaluate in vitro antagonism to Pyricularia oryzae. Fungal isolates of B. oldhamii shoots were purified and identified by nuclear ribosomal DNA (nrDNA) including internal transcribed spacer regions (ITS1 and ITS4). These isolates were then confirmed by phylogenetic analysis of the ITS1 and ITS2 sequences and morphological analysis. The isolates were evaluated in in vitro experiments of direct and indirect antagonism (volatile production). Five fungal isolates were identified and named as follows: isolate 27 (Arthrinium sp.); isolate 29 (Acrocalymma sp.); isolate 122 (Botryobambusa fusicoccum); isolate 711 (Phoma sp.) and isolate 712 (Phoma sp.). We found that the area of the P. oryzae colony was reduced by more than 80% by all endophytic isolates in this study, with emphasis on isolate 122, with a 96% reduction in area of the P. oryzae. These results were considered promising and will serve as a foundation for future studies on induction of resistance to P. oryzae.

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In vitro culture and diversity of endophytic fungi in Bambusa oldhamii

Abstract: RESUMO

Cited by 9 publications

References 22 publications

ROLE OF DIMETHYL SULFATE ON BIOCHEMICAL CHARACTERISTICS OF Fragaria ananassa Duch UNDER SALINITY STRESS IN VITRO

ROLE OF DIMETHYL SULFATE ON BIOCHEMICAL CHARACTERISTICS OF Fragaria ananassa Duch UNDER SALINITY STRESS IN VITRO

In vitro establishment from mature nodal segments of Persian Lime (Citrus latifolia Tan.)

Isolation, identification and characterization of endophytic fungi of Bambusa oldhamii munro applied as antagonists to Pyricularia oryzae

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