In Vitro Culture Initiation and Cryopreservation of Endemic Taxa Dianthus Giganteus Ssp. Banaticus

Jarda, Liliana; Cristea, Vasile; Halmágyi, Adela; Plada, M.

doi:10.17660/actahortic.2011.918.18

Cited by 8 publications

(6 citation statements)

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“…There are few data about molecular analysis of rare or endemic Dianthus species (Cristea et al 2014, Jarda et al 2014 despite the numerous data regarding the conservation of these species. In Romania these plant species were preserved by in vitro cultures (Butiuc-Keul et al 2001, Miclăuş et al 2003, Marcu et al 2006, Jarda et al 2011. Our study revealed the genetic diversity structure of 5 endangered or endemic species of Dianthus found in different sites in Romania.…”

Section: Genetic Diversity Of Romanian Dianthus Taxamentioning

confidence: 54%

Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

Butiuc-Keul¹,

Crăciunaş²,

Goia³

et al. 2018

Acta Botanica Croatica

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In order to develop a proper conservation programme for several endangered, rare or endemic species of Dianhtus from Romania, molecular characterization by simple sequence repeat (SSR) markers has been accomplished. Amplification of SSR loci in individuals belonging to different populations of D. callizonus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis and D. tenuifolius revealed 23 polymorphic alleles. D. callizonus and D. tenuifolius showed particular sets of SSR alleles. D. glacialis ssp. gelidus, D. henteri and D. nardiformis proved to share almost the same alleles in most of the loci. The highest genetic diversity was observed in D. glacialis ssp. gelidus and D. tenuifolius in locus MS-DINMADSBOX. Allelic patterns across Dianthus species indicate that the mean number of different alleles was highest in D. glacialis ssp. gelidus, while the number of effective alleles was highest in D. tenuifolius. There are no particular differences in individuals belonging to the same species. Genetic diversity is generally low, ranging from 0.18 (D. callizonus) to 0.44 (D. henteri). Regarding the genetic diversity within populations of the same species, no differences were revealed by the use of the SSR markers tested in the present study.

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Section: Genetic Diversity Of Romanian Dianthus Taxamentioning

confidence: 54%

Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

Butiuc-Keul¹,

Crăciunaş²,

Goia³

et al. 2018

Acta Botanica Croatica

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“…They obtained 8 shoots/inoculum after 56 day of culture on medium with K and NAA (1.0 mg/L of each). Previous observations at 30 and 60 days from the transfer on D2 medium suggest that after 30 days the plants from all 3 populations multiplied relatively uniformly, but after 60 days there is an obvious difference between the populations (Jarda et al, 2011). A high multiplication rate (110-240 neoshoots/inoculum) after 83 days of culture on medium supplemented with BAP (1.0 mg/L) and NAA (0.1 mg/L) was also obtained for another strictly endemic species in the South Carpathians, D. henteri Heuff.…”

Section: In Vitro Multiplicationmentioning

confidence: 80%

“…For the initiation of in vitro culture of D. giganteus subsp. banaticus taxa, plant material (nodal explants prelevated from in situ plants, 20 explants/individuals/sterilizing methods) was sterilized using several methods, as described by Jarda et al (2011). Before sterilization the plant material was pre-sterilized by washing in water, rinsing in 5% Domestos, and a short rinse in 80% ethylic alcohol.…”

Section: Plant Materialsmentioning

confidence: 99%

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Jarda¹,

Butiuc-Keul²,

Höhn³

et al. 2014

Turk J Biol

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Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

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“…Тако је, код D. giganteus ssp. banaticus испитивана могућност криопрезервације (Jarda et al, 2011), а код D. spiculifolius и D. glacialis ssp. gelidus утврђен је протокол за успостављање банке гена (Holobiuc et al, 2009), при чему је позитиван ефекат имало додавање манитола као осмотског фактора у медијум (Butiuc-Keul et al, 2001, Cristea et al, 2002, Holobiuc et al, 2010b, Pop i Pamfil, 2011.…”

Section: размножавање угрожених врста каранфилаunclassified

“…However, there were some additional researches. Thus, Jarda et al (2011) investigated the possibility of cryopreservation of D. giganteus ssp. banaticus, and Holobiuc et al (2009) determined the protocol for the establishment of a gene bank of D. spiculifolius and D. glacialis ssp.…”

Section: Moroieni Romaniamentioning

confidence: 99%

In vitro propagation of endangered Dianthus taxa

Marija¹,

Mihailo²,

Đukić³

2015

Glas Suma Fakul

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The review of recent researches regarding the in vitro culture of 30 endangered Dianthus taxa is presented in this paper. Various in vitro protocols developed for selected rare and threatened Dianthus taxa are analysed in order to provide a useful synthesis of the data obtained with the main principles, techniques and recommendations for futher research and practice. The recapitulated data presented in this review can be used as a tool for the micropropagation of other endangered Dianthus taxa, enabling their propagation and obtaining a sufficient amount of plants for reintroduction. In addition, the obtained results represent the basis for ex situ conservation of the investigated taxa, especially for medium-term and long-term conservation (cryopreservation). [Projekat Ministarstva nauke Republike Srbije, br. 43007]

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In Vitro Culture Initiation and Cryopreservation of Endemic Taxa Dianthus Giganteus Ssp. Banaticus

Cited by 8 publications

References 0 publications

Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

In vitro propagation of endangered Dianthus taxa

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