In vitro culture of albedo tissue from fruits of Citrus sinensis cv. Washington Navel: Effect of fruit age and orange juice

Amo-Marco, J. B.; Picazo, I.

doi:10.1080/14620316.1994.11516529

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Similar results were obtained in other studies related to adding of plant extracts juice of coconut, tomato, potato, onion, banana, orange, apple, pineapple and yeast to the culture medium [7,9,10,11,18] . As indicated previously, most of the vegetative growth characteristics measured becomes enhanced with increasing date syrup in the medium up to 6%.…”

Section: Resultssupporting

confidence: 90%

“…It has been reported that in many plant species adding of plant extracts juice of coconut, tomato, potato, onion, banana, orange, apple, pineapple and yeast to the culture medium enhanced the growth of tissues [7][8][9][10][11] . Kinnersley and Henderson [12] found that addition of corn (maize) syrup to basic culture media improves embryogenesis of wild carrot.…”

Section: Introductionmentioning

confidence: 99%

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Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Al-Khateeb¹

2008

American J. of Biochemistry and Biotechnology

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Abstract:The effect of different concentration of date palm syrup (2, 4, 6, 8 and 10%) and sucrose at concentration of 30 and 60 g/l in addition to the control (without carbon source) on the micro propagation of date palm "cv. Suckary" were investigated. The results indicated that date syrup was taken up from the media as shown by the increase in total dry weight of culture. Addition of sucrose at 60 g/l produced the highest number of somatic embryos and longest shoot equal to that produced by date syrup at concentration of 6% without significant differences. On the other side, sucrose at 30 g/l improved the germination of somatic embryos followed by date syrup at 6%. Regarding fresh and dry weights, adding 6% date palm syrup was superior in this respect. Tissues grown on medium supplement with 10% of date syrup caused severe reduction in number of somatic embryo possibly due to the osmotic stress. In addition, tissues grown on medium containing 2% of date syrup had the lowest number of embryos. Date palm tissues are capable of utilizing date syrup as the sole carbon source for vegetative growth. It can be concluded that, adding date syrup locally known as 'Dibs' to the culture medium of date palm at concentration of 6% can be used totally as a replacement of sucrose which was the normally sugar used in most of plant tissue culture medium. This is the first report on the use of date syrup on somatic embryogenesis of date palm cv. Suckary.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Al-Khateeb¹

2008

American J. of Biochemistry and Biotechnology

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“…It seems no relation of fruit weight, fruit diameter, ovule weight, ovule length and ovule diameter to nucellar embryogenesis responses. However, it is reported in Washington navel, the growth of albedo calk was dependent on the 20% juice of the specific age of fruit with diameter 3045 mm (Amo-Marco and Picazo, 1994). In fruit species My icaria cauliflora, ovule diameter have 64% explant callusing and ovule diameter (0.8 cm) have 31% embryogenic cultures (Litz, 1984).…”

Section: Resultsmentioning

confidence: 98%

Effect of Radiation on Nuclear Embryogenesis in Sweet Orange Cultivars

Altaf¹,

Khalil²,

Marwat³

et al. 2000

Pakistan J. of Biological Sciences

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All the Thirteen sweet orange cultivars which were tested for nuclellus embryos in MS + BA (0.5 mg/I) + Glutamine 5 rrigil responded to embryogenesis. After two months culture period, the maximum (7.0) coefficient of nuclellar regeneration (CNR) was of cultivar Mosambi, which has minimum ovvule weight (0.11 cm) and the minimum CNR was of Moro blood (2.0). The fruit characteristics like fruit weight, diameter, ovule weight, length and diameter have no relations with CNR. The maximum values of CNR were in

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“…At this concentration, response for primary callus induction was 88% (Al Taha et al 2013). Optimal callus induction response was observed on MS medium, supplemented with1.5 mg/L 2, 4-D from all types of explants, with highest response (92%) and maximum shoot regeneration response (70 %) from callus incubated on MS medium supplemented with BA 3 mg/L [67 and at the same time in genus C. sinensis, MS medium supplemented with orange juice stimulated callus growth [68] whereas in Mexican lime, embryogenic calli was induced by 0.5mg/L 2,4 D supplemented MS medium [69]. Among the three explants such as nodal segments, leaf and root segments, nodal segments had been considered as the best explants for induction of callus since calli derived from nodal segments were green and friable as compared to leaf and root segments while callus derived from the leaf segments appeared to be brown and necrotic and therefore it had been confirmed that response to callus induction depended on the type of explants as well as concentration and the type of plant growth regulator used [70].…”

Section: Callus Inductionmentioning

confidence: 99%

Recent Advances of Biotechnological Tools on Diverse Species of Citrus: Current Applications and Future Prospects

Sharma

Roy

2021

IJPSS

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Based on the long term conservation of several endangered and indigenous species of Citrus, significant impact of biotechnological tools particularly in terms of in-vitro micropropagation methods in addition to synthetic seed production using encapsulation of plant propagules including shoot tips, nodal segments, androgenic embryos, embryogenic callus, etc. in sodium alginate has been highlighted in this manuscript. When seed is not available in enough quantity for raising seedlings for rootstock or have low levels of polyembryony and do not produce adequate quantities of nucellar seedlings, then micropropagation techniques could quickly supply in vitro regenerated rootstock or budwood. Rapid, mass-production and cost-effective biotechnological tools for propagation of citrus rootstocks and budwood would be of great importance in this regard. Reports on another aspect of long term conservation particularly based on storage of cells, tissues and organs of drought tolerant species of Citrus at ultra-low temperature preferably at -196 ºC via applications of cryopreservation techniques using vitrification and encapsulation or dehydration methods has been highlighted in this manuscript. In addition, several research on techniques of in-vitro micrografting using superior scion and rootstocks of two different species of Citrus with an objective of eradication of virus infected citrus stocks for successful production of grafts have been reviewed. Furthermore, effects of explants either through direct and indirect regeneration and conversion into a complete disease free plantlet using suitable synthetic nutrient media along with plant growth regulators at various concentrations and combinations have been highlighted in this manuscript. Hence, the current review is primarily focused on the applications and its effects of superior biotechnological tools for long term conservation of diverse species of citrus for further increasing the potentiality of Citrus industries in addition to genetic improvement and genetic resource conservation.

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In vitro culture of albedo tissue from fruits of Citrus sinensis cv. Washington Navel: Effect of fruit age and orange juice

Cited by 5 publications

References 15 publications

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Effect of Radiation on Nuclear Embryogenesis in Sweet Orange Cultivars

Recent Advances of Biotechnological Tools on Diverse Species of Citrus: Current Applications and Future Prospects

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