In Vitro culture of Eucalyptus grandis: Effect of gelling agents on propagation

Macrae, S.; Staden, J. Van

doi:10.1016/s0176-1617(11)80092-1

Cited by 24 publications

(7 citation statements)

References 4 publications

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“…The control plants were grown on soil without AC in a growth chamber where light intensity was 221 lmol m -2 s -1 provided by fluorescent and incandescent lighting propagating walnut (Juglans nigra) (Driver and Kuniyuki 1984), both of these media were not well-suited for growing 'Nisqually-1'. Nadel (1992) also reported that MS medium was a suitable medium, although less effective than -strength MS. Gelling agents can significantly affect the performance of in vitro culture (MacCrae and Van Staden 1990;Cheng and Shi 1995). The performance of 'Nisqually-1' in agar and Gelrite media were similar to that observed with Siberian elm [Ulmus pumila (Cheng and Shi 1995)], where shoots in agar-solidified medium deteriorated in 1 week, but fully recovered when transferred to Gelrite medium, while those in Gelrite medium deteriorated in 1 week after being transferred to agar-gelled medium.…”

Section: Discussionsupporting

confidence: 54%

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Kang

Osburn

Kopsell

et al. 2009

Plant Cell Tiss Organ Cult

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Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populus trichocarpa (Torr. & Gray) genotype 'Nisqually-1'. However, 'Nisqually-1' has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1' was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulatorfree Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0.3% Gelrite Ò and 5-10 g L -1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

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Section: Discussionsupporting

confidence: 54%

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Kang

Osburn

Kopsell

et al. 2009

Plant Cell Tiss Organ Cult

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“…However, the possible stimulatory effect of Gelrite can not be excluded, and this has been reported in a number of herbaceous and woody species (e.g. MacRae and van Staden, 1990;Mii et al, 1991). Siberian elm may be sensitive to some micro-elements present in agar that are absent in Gelrite, e.g.…”

mentioning

confidence: 99%

Micropropagation of mature siberian elm in two steps

Cheng

Shi

1995

Plant Cell Tiss Organ Cult

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A simple and reliable two-step micropropagation system was developed for mature Siberian elm (Ulmus pumila L.). Shoot-tip cultures were initiated and multiplied on a modified Murashige and Skoog medium supplemented with 1 ~tM benzyladenine, B5 vitamins, solidified with 0.22% Gelrite, and subcultured weekly. Proliferated shoots 2-3 cm in length rooted successfully in sterile and non-sterile soil mix at 100% efficiency. Healthy plants were acclimatized to the ambient environment.

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“…To prevent precipitation, agar could be used in place of Phytagel as the solidifying agent for many species, but this cannot be done with Siberian elm (Cheng and Shi, 1995) and some other species, such as Eucalyptus grandis Anth. (MacRae and van Staden, 1990), because agar inhibits shoot development in these species. In these situations, precipitation can be easily corrected by adjusting the pH of antibiotic stock solutions to 9 or 10.…”

Section: Discussionmentioning

confidence: 99%

Aminoglycoside Antibiotics Inhibit Shoot Regeneration from Siberian Elm Leaf Explants

Kapaun¹,

Cheng²

1999

HortSci

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Four aminoglycoside antibiotics were evaluated for their effects on shoot regeneration from leaf explants of Siberian elm (Ulmus pumila L.) seedlings and their potential use as selective agents in genetic transformation with the neomycin phosphotransferase II gene as the selective marker gene. Kanamycin at 100 mg·L–1 or higher concentration reduced shoot regeneration, with complete inhibition at 225 mg·L–1, and was considered a suitable selective agent. Neomycin completely inhibited shoot regeneration at 450 mg·L–1, but all explants remained green; therefore, it may also be used as a selective agent. Geneticin significantly inhibited shoot formation at 1 mg·L–1 and completely killed the explants at 4 mg·L–1 after 1 week. Geneticin was too toxic for direct selection, but may be useful in a delayed selection scheme or for confirmation of transformation. Paromomycin was least effective in inhibiting shoot formation; 13% of explants still regenerated shoots on the medium with the highest concentration tested (400 mg·L–1). Both neomycin and paromomycin precipitated in media containing Phytagel as a gelling agent if antibiotic stock solutions were added to the medium without adjusting their pH. Precipitation was prevented by adjusting the pH of the stock solutions from 6.2 (neomycin) or 6.9 (paromomycin) to above 9, or by using agar as a gelling agent. The precipitation was not affected by the concentrations of salts in the media.

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In Vitro culture of Eucalyptus grandis: Effect of gelling agents on propagation

Cited by 24 publications

References 4 publications

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Micropropagation of mature siberian elm in two steps

Aminoglycoside Antibiotics Inhibit Shoot Regeneration from Siberian Elm Leaf Explants

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