Micropropagation of mature siberian elm in two steps

Cheng, Zong‐Ming; Shi, Nian‐Qing

doi:10.1007/bf00051592

Cited by 17 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, Bonga and Von Aderkas (1992) mention that, for many hardwoods, roots formed in vitro had enlarged cortical cells and underdeveloped vascular systems and thus were of poorer quality than roots formed ex vitro. The same conclusion was drawn by Cheng and Shi (1995), who reported that the in vitro rooting stage could be eliminated to reduce time and cost.…”

Section: Tip Segmentssupporting

confidence: 78%

“…In our work, acclimatization was given particular attention. Two approaches, similar to those tested by Cheng and Shi (1995) with U. pumila, were assayed: in vitro rooting prior to transfer to soil mixture (Fig. 1c, d) and direct transfer of shoots to soil mixture (Fig.…”

Section: Tip Segmentsmentioning

confidence: 99%

“…Gartland and co-workers obtained genetically modified elms in 2001 (GM trees fight DED 2001) and in 2005 they reported that these elms were being tested for their resistance to the fungus (Gartland et al 2005). Other reports on elm micropropagation were carried out (Cheng and Shi 1995;Corchete et al 1997) and a significant number of these involved shoot production using leaves as primary explants (Bolyard et al 1991b;George and Tripepi 1994;Kapaun and Cheng 1997;Ben Jouira et al 2000). Recently, micropropagation of U. glabra was carried out with dormant axillary buds as primary explants (Biroscíková et al 2004).…”

mentioning

confidence: 96%

See 2 more Smart Citations

A protocol for Ulmus minor Mill. micropropagation and acclimatization

Conde

Sousa

Costa

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Here we report the establishment of a simple protocol for the micropropagation and acclimatization of U. minor. Branches with dormant buds were collected from mature elms and sprouted in a greenhouse. Tip and node segments were used as starting material for in vitro proliferation in a medium (designated here as DKW1) already used for the micropropagation of a clone of the English Elm (U. procera SR4). In the first assay, in which explants from nine different trees were used, 88.5% of the tip segments produced new axillary shoots thus proving to be the best explant type. Afterwards, material from four different trees (F4, F7, F13, F14), that had the highest sprouting rate in the greenhouse, was used to test for genotype influence. F14 proved to be the best genotype in culture and it was used for all the subsequent experiments. Shoots from F14 were used to assay in vitro rooting using five DKW based media. Rooting percentages were high for all media and varied between 80% and 100%. For acclimatization two approaches were assayed: the use of previously rooted in vitro plants and the direct acclimatization of shoots from cultures in DKW1. After 6 weeks, 86.4% of the in vitro rooted plants were successfully acclimatized and a slightly higher value, 88.6%, was attained by direct acclimatization of shoots with thick stems and hard leaves. These results proved that there is no need for a previous in vitro rooting step and that direct acclimatization can effectively reduce time and costs. Thus U. minor micropropagation and acclimatization can be divided into only two steps: proliferation of shoots in DKW1 and direct acclimatization of these shoots in a sterile soil mixture.

show abstract

Section: Tip Segmentssupporting

confidence: 78%

Section: Tip Segmentsmentioning

confidence: 99%

mentioning

confidence: 96%

See 1 more Smart Citation

A protocol for Ulmus minor Mill. micropropagation and acclimatization

Conde

Sousa

Costa

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…The control plants were grown on soil without AC in a growth chamber where light intensity was 221 lmol m -2 s -1 provided by fluorescent and incandescent lighting propagating walnut (Juglans nigra) (Driver and Kuniyuki 1984), both of these media were not well-suited for growing 'Nisqually-1'. Nadel (1992) also reported that MS medium was a suitable medium, although less effective than -strength MS. Gelling agents can significantly affect the performance of in vitro culture (MacCrae and Van Staden 1990;Cheng and Shi 1995). The performance of 'Nisqually-1' in agar and Gelrite media were similar to that observed with Siberian elm [Ulmus pumila (Cheng and Shi 1995)], where shoots in agar-solidified medium deteriorated in 1 week, but fully recovered when transferred to Gelrite medium, while those in Gelrite medium deteriorated in 1 week after being transferred to agar-gelled medium.…”

Section: Discussionsupporting

confidence: 72%

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Kang

Osburn

Kopsell

et al. 2009

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populus trichocarpa (Torr. & Gray) genotype 'Nisqually-1'. However, 'Nisqually-1' has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1' was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulatorfree Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0.3% Gelrite Ò and 5-10 g L -1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

show abstract

“…However, this strategy can only be initiated after the establishment of efficient protocols for plant regeneration from cells and tissues of elm trees (Fenning et al 1996a). Protocols of this type have already been developed from differentiated explants (Fink et al 1986;Dorion et al 1987;Bolyard et al 1991;Fenning et al 1993;George and Tripepi 1994;Cheng and Shi 1995;Kapaun and Cheng 1997;Ben Jouira et al 1998), from callus (Karnosky et al 1982), from suspension cultures (Durzan and Lopushansky 1975) and from protoplasts (Sticklen et al 1986;Dorion et al 1994) of some species and clones of special interest.…”

Section: Introductionmentioning

confidence: 97%

Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill.

2004

View full text Add to dashboard Cite

Somatic embryogenesis from mature elm ( Ulmus minor Mill.) in vitro-cloned material is possible. Embryogenic callus was obtained from leaves inoculated on two different MS-based media-one supplemented with 2.3 microM 2,4-dichlorophenoxyacetic acid (I2) and the other supplemented with 1.1 microM kinetin (I6). However, only leaves cultured on medium I6 produced somatic embryos, at the globular stage, when embryogenic callus was maintained in induction media. When embryogenic callus from medium I6 was transferred to basal medium, somatic embryos with green cotyledons were obtained. An average of 35.9% of these embryos converted easily into normal plants in conversion medium with 1% sucrose. Acclimatisation reached 39.7%, and this was not significantly different from a control group consisting of plants propagated by axillary buds. No morphological differences were observed between plants derived from somatic embryos and control plants. Also, no differences in ploidy were detected between the somatic embryo-derived plants and the mother plants.

show abstract

Micropropagation of mature siberian elm in two steps

Cited by 17 publications

References 9 publications

A protocol for Ulmus minor Mill. micropropagation and acclimatization

A protocol for Ulmus minor Mill. micropropagation and acclimatization

Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill.

Contact Info

Product

Resources

About