In-Vitro Culture of Human Chondrocytes From Adult Subjectsm

Delbrück, A.; Dresow, Bernd; Gurr, Eberhard; Reale, E.; Schröder, Heinrich

doi:10.3109/03008208609167140

Cited by 28 publications

(13 citation statements)

References 30 publications

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“…This result confirms the findings of Delbruck et al (1986). The proteoglycans synthesized in vitro were identical to those of the native cartilage, with respect to molecular weight and length of glycosaminoglycan chains.…”

Section: Esupporting

confidence: 81%

“…drocyte culture methods were developed primarily using animal tissues, as in studies of limb development (Solursh et al, 1986), and have now been applied to human cells. Delbruck et al (1986) showed that human articular cells in agarose culture produced type I1 collagen. Synthesis of type II collagen and cartilage-specific proteoglycans by chondrocytes has been shown by Bassleer et al (1986).…”

Section: Discussionmentioning

confidence: 99%

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Proteoglycan synthesis by cultured human chondrocytes

Aulthouse

Becker²,

Beck

1994

Microscopy Res & Technique

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Iliac crest biopsies are important in the detection of human skeletal dysplasias. Therefore, culture of these cells may serve as a valuable method for studying proteoglycan metabolism in chondrocytes of individuals with skeletal abnormalities. Morphological and biochemical studies were performed on human iliac crest chondrocytes grown in monolayer and in agarose gels. Two proteoglycan populations of different hydrodynamic size and glycosaminoglycan composition were synthesized by cells grown in monolayer. Chondrocytes cultured in an agarose gel for 2 weeks synthesized proteoglycans identical to those of the native tissue with respect to hydrodynamic size and glycosaminoglycan chain length. However, the ratio of chondroitin-6-sulfate to chondroitin-4-sulfate was higher than in the native tissue. This ratio was not influenced by different sulfate concentrations in the medium. Moreover, treatment with ascorbic acid did not influence proteoglycan synthesis; however, there was a pericellular accumulation of proteoglycans.

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Section: Esupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Proteoglycan synthesis by cultured human chondrocytes

Aulthouse

Becker²,

Beck

1994

Microscopy Res & Technique

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“…For organ cultures pieces of cartilage of equal size were embedded in 0.75°/0 low-gellingtemperature agarose and cultured in Opti-MEM 1 supplemented with 10070 FCS, 10/0 Ultroser G, 1 #g/ml insulin and 50/zg/ml ascorbic acid. For agarose cultures chondrocytes were isolated by collagenase treatment essentially as described [20,21]. Culture medium was the same as described above.…”

Section: In Vitro Culture Of Human Chondrocytesmentioning

confidence: 99%

Interleukin‐1β induces synthesis and secretion of interleukin‐6 in human chondrocytes

et al. 1990

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Increased concentrations of interleukin‐6 (IL‐6) have been found in the synovial fluid of patients with osteoarthritis, rheumatoid arthritis and crystal‐related joint deseases. It is therefore of great interest to identify the cells responsible for the production of IL‐6, and to investigate whether IL‐6 plays a role in the pathogenesisof degenerative or inflammatory joint diseases. Here we show that human interleukin‐1β (IL‐1β) induces IL‐6 synthesis and secretion in differentiated human chondrocytes. In organ cultures resembling closely the in vivo system 106 chondrocytes incubated with 100 units of interleukin‐1β per ml of medium led to the release of 6 × 103 units of IL‐6 within 24 h. Chondrocytes cultured in agarose or as monolayers similary incubated with IL‐1β produced even higher amounts of IL‐6: 70 × 103 units per 106 cells within 24 h. The induction of IL‐6 synthesis by IL‐1β was also shown at the mRNA level. IL‐6 secreted by stimulated chondrocytes showed heterogeneity upon Western blot analysis.

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“…Hence, the genes that are strong candidates for sites of mutations that cause chondrodysplasias are not expressed in vitro. However, modu lated epiphyseal chondrocytes from many species reexpress the phenotype of the typi cal chondrocyte if cultured in or on agarose gels [36][37][38][39]. Furthermore, in the chick at least, such '^differentiated' chondrocytes subsequently express the phenotype of the hypertrophic chondrocyte if maintained in agarose culture [37,38].…”

Section: In Vitro Studiesmentioning

confidence: 99%

Approaches to Investigating Growth Plate Cartilage in the Human Chondrodysplasias

Horton¹

1988

Path Immunopathol Res

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In-Vitro Culture of Human Chondrocytes From Adult Subjectsm

Cited by 28 publications

References 30 publications

Proteoglycan synthesis by cultured human chondrocytes

Proteoglycan synthesis by cultured human chondrocytes

Interleukin‐1β induces synthesis and secretion of interleukin‐6 in human chondrocytes

Approaches to Investigating Growth Plate Cartilage in the Human Chondrodysplasias

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