In vitro culture of Perkinsus atlanticus, a parasite of the carpet shell clam Ruditapes decussatus

Ordás, M. Camino; Figueras, A.

doi:10.3354/dao033129

Cited by 29 publications

(20 citation statements)

References 15 publications

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“…The formation of prezoosporangia in RFTM and the development of zoospores in seawater were similar to those of P. marinus (Perkins, 1996), P. olseni (Lester and Davis, 1981), and P. atlanticus (Azevedo, 1989;Auzoux-Bordenave et al, 1995;Ordas and Figueras, 1998 …”

Section: Discussionmentioning

confidence: 74%

Occurrence of Perkinsus Species (Protozoa, Apicomplexa) from Manila Clam Tapes philippinarum in Japan.

Maeno¹,

Yoshinaga²,

Nakajima³

1999

Fish Pathol.

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Section: Discussionmentioning

confidence: 74%

Occurrence of Perkinsus Species (Protozoa, Apicomplexa) from Manila Clam Tapes philippinarum in Japan.

Maeno¹,

Yoshinaga²,

Nakajima³

1999

Fish Pathol.

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“…In some cases, protozoans that were initially described as Perkinsus spp. based partly on their morphologies were later found not to belong in the genus Perkinsus (McGladdery et al 1991, Goggin et al 1996, Ordás & Figueras 1998, Figueras et al 2000. Currently, as mentioned above, classification of P. qugwadi, is questionable because of noted differences in morphology from that which is the typical morphology of Perkinsus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Further evidence that P. atlanticus belongs to the genus Perkinsus was provided from nucleotide sequence analysis of the small subunit (SSU) rRNA gene and the internal transcribed spacer (ITS) region of the rRNA gene complex (Goggin 1994, de la Herrán et al 2000, Robledo et al 2000, Casas et al 2002. Recently, Ordás & Figueras (1998) reported the in vitro culture of P. atlanticus from the haemolymph of an infected carpet shell clam. Identification of the cultured cells was based solely on their morphology (light and electron microscopy).…”

Section: Introductionmentioning

confidence: 99%

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Continuous in vitro culture of the carpet shell clam Tapes decussatus protozoan parasite Perkinsus atlanticus

Casas¹,

Peyre²,

Reece³

et al. 2002

Dis. Aquat. Org.

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Continuous in vitro cultures of the clam Tapes decussatus parasite Perkinsus atlanticuswere established from infected gill fragments, infected haemolymph and parasite hypnospores isolated from infected gill fragments following incubation in Ray's fluid thioglycollate medium (RFTM). No continuous cultures could be initiated from P. atlanticus zoospores. Cultures initiated from hypnospores yielded the highest percentage of continuous cultures (100%, 6/6), followed by cultures initiated from gill fragments (93%, 43/46) and from haemolymph (30%, 3/10). Failures to establish continuous cultures were due to microbial contamination. The source of parasite influenced the success rate, the time taken to establish cultures and the size of cultured cells. In vitro proliferation of parasite cells was mainly by vegetative multiplication. Zoosporulation, yielding motile biflagellated zoospores, was observed at a low frequency (<1% of dividing cells) in every culture. Morphology of cultured cells examined with light and transmission electron microscopy corresponded to that of P. atlanticus found in clam tissues. Cultured cells enlarged in RFTM and stained blue-black with Lugol's solution, which are characteristics of the Perkinsus species cells. DNA sequences of the internal transcribed spacer (ITS) region of the ribosomal RNA gene complex matched those of P. atlanticus. All cultures were established in a medium designated JL-ODRP-2A that was similar in composition to the culture medium JL-ODRP-1 originally used to propagate Perkinsus marinus in vitro. Proliferation of P. atlanticus in vitro could be supported by the commercial culture medium (1:2 v/v) DME:Ham's F-12 with fetuin. KEY WORDS: Perkinsus atlanticus · Tapes decussatus · In vitro culture · Clam parasite · Ribosomal RNA gene complex · ITS · UltrastructureResale or republication not permitted without written consent of the publisher Dis Aquat Org 52: [217][218][219][220][221][222][223][224][225][226][227][228][229][230][231] 2002 spacer (ITS) region of the rRNA gene complex (Goggin 1994, de la Herrán et al. 2000, Robledo et al. 2000, Casas et al. 2002. Recently, Ordás & Figueras (1998) reported the in vitro culture of P. atlanticus from the haemolymph of an infected carpet shell clam. Identification of the cultured cells was based solely on their morphology (light and electron microscopy). Sequence analysis of the SSU small subunit rRNA gene, however, showed that those cultured cells did not correspond to a Perkinsus organism (Figueras et al. 2000). Continuous cultures of P. atlanticus, therefore, still need to be established.The development of continuous cultures of Perkinsus species can lead to a better understanding of this group of parasites . Cultures of the eastern oyster pathogen Perkinsus marinus, for example, have been used in a wide range of studies to address the parasite's environmental tolerance (Burreson et al. 1994, Dungan & Hamilton 1995, virulence , Bushek & Allen 1996a, Volety & Chu 1997, genetic composition (Reece et al. 1997, Reece et...

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“…Pseudoperkinsus tapetis cells, cultured as described by Ordás & Figueras (1998), were centrifuged and washed twice in ASW. Then, cellular suspensions with concentrations 10 7 , 10 6 , 10 5 , 10 4 and 10 3 cells ml 1 were prepared in ASW and added to the vials as stimuli.…”

Section: Methodsmentioning

confidence: 99%

Modulation of the chemiluminescence response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes

Ordás

Novoa

Figueras

2000

Fish & Shellfish Immunology

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In vitro culture of Perkinsus atlanticus, a parasite of the carpet shell clam Ruditapes decussatus

Cited by 29 publications

References 15 publications

Occurrence of Perkinsus Species (Protozoa, Apicomplexa) from Manila Clam Tapes philippinarum in Japan.

Occurrence of Perkinsus Species (Protozoa, Apicomplexa) from Manila Clam Tapes philippinarum in Japan.

Continuous in vitro culture of the carpet shell clam Tapes decussatus protozoan parasite Perkinsus atlanticus

Modulation of the chemiluminescence response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes

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