In vitro Culture of Somatic Wheat Callus Tissue

Trione, E. J.; Jones, Leo E.; Metzger, R. J.

doi:10.2307/2440606

Cited by 20 publications

(3 citation statements)

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“…Shoots wcrc only rarely redifferentiated from callus of corninoil wheat -(a total of only six cases). Using the same materials, Trione et al (1968) reported no su~cessful shoot formation. On the contrary, shoot rediffcrentiation from the callus tissucs was found to be rather easy in oats by Carter et nl.…”

Section: Orgnn Rediffel-e;ltiatio?z From Cnllzlsmentioning

confidence: 88%

In Vitro Culture of Wheat Tissues. I. Callus Formation, Organ Redifferentiation and Single Cell Culture

Shimada¹,

Sasakuma²,

Tsunewaki³

1969

Can. J. Genet. Cytol.

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Callus induction, organ formation from callus and single callus cell culturc have bcen tried in wheat. Though lcinetin sl~owed no effects, supplemcnts of 2,4-1) (I-lOnig/l) or IAA (jOmg/l) to tlie basal mcdia induced calluses from seedling roots of einl~orn, enlmcr and coninion wtlcats, and from stem pieccs of common \vheat. T h e best caIIus gron;th was olxained when casein liydroIysate ( l g / l ) or coconut milk (1%) was added t o tlic basal media. Callus gl.owtIi n.as also vigorous when 2,4-11 (0.5-2.0rng/l) uras added. Root formation from callus took place in all kinds of tested media, except those containing no growth factors or supplemented \vitli 2,4-D at high concentrations (I-jmg/l).Shoot formation occurred in six cascs and no growth factors wcre found to be specifically cffective on shoot differcntiation. Tv.0 plants were rcstorcd and rcaclictl maturity. Calluses of coninion wheat consisted of eudiploid and ancuploid cells at almost the same frequencies. T h e grcat majority of ancuploid cells had 42 5 3 cliromosonies. T h e restored plants slio\ved normal chromosome constitution. Single callus-cell suspensions \\.ere obtained. b! . thc liquid culture of seeds in a shalcer. A filtrate of tlic single cell suspension was plated o11 a solid agar ti~edi~uii, and some colonies ufcrc formed. Ho\\.cver, plating efficiency uras very l o u~ and colony grou~th was slow.

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Section: Orgnn Rediffel-e;ltiatio?z From Cnllzlsmentioning

confidence: 88%

In Vitro Culture of Wheat Tissues. I. Callus Formation, Organ Redifferentiation and Single Cell Culture

Shimada¹,

Sasakuma²,

Tsunewaki³

1969

Can. J. Genet. Cytol.

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“…Materials and Methods Callus and cell suspension cultures of wheat have been obtained from several tissues (Trione et al 1968; Gamborg and Eveleigh 1968). The cultures were derived from tissues of roots and germinating seeds.…”

Section: Introductionmentioning

confidence: 99%

Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures

Dudits¹,

Nemet²,

Haydu³

1975

Can. J. Bot.

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DUDITS, D., G. NEMET, and Z. HAYDU. 1975. Study of callus growth and organ formation In wheat (Triticlrtn aestii~rrtn) tissue cultures. Can. J. Bot.153: 957-963. Callus cultures of wheat (Tritic~rm aestivlrtn L.) were established by incubation of segments from root tips, shoots of seedlings, and from rachis with B5 and T media. 2,4,5-Trichlorophenoxyacetic acid, Benazolin, and Banvel D (Dicamba) were found to be appropriate growth regulators for initiation and maintenance of wheat callus cultures. Cytokinins inhibited callus growth. This effect was less pronounced with zeatin than with kinetin and benzyladenine. Supplementation of media with cytokinins, however, increased the number of roots formed in the callus. Shoots and complete plants were regenerated from rachis and shoot callus. DUDITS, D., G. NEMET et Z. HAYDU. 1975. Study of callus growth and organ formation in wheat (Triticum aestivlrm) tissue cultures. Can. J. Bot. 53: 957-963. Des cultures de callus de ble (Triticum aestivum L.) ont et6 obtenues en incubant sur les milieux B5 et T des segments d'extrkmites de racines et de tiges de plantules, ainsi que de rachis.L'acide 2,4,5-trichlorophCnoxy-acetique, la Benazoline et le Banvel D (Dicamba) se sont avCrCs des rkgulateurs de croissance efficaces pour assurer I'initiation et le maintien des cultures de callus de blC. Les cytokinines inhibaient la croissance des callus. Cet effet Ctait moins prononcC avec la zeatine qu'avec la kinetine et la benzyladtnine. Cependant, I'addition de cytokinines dans les milieux augmentait le nombre de racines formees dans le callus. Des tiges et des plantes complktes se sont r6gCnCrkes partir de callus de rachis et de tiges.[Traduit par le journal]

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“…Nodal as well as internodal segments are commonly used for induction of morphogenic callus cultures in dicots, but there are only a few brief reports available for cereals mentioned in the earlier phase of development of tissue culture methods. In attempts to initiate regenerable callus in wheat and barley, nodes were among the different explants analysed but without success (Trione et al 1968;Wilson et al 1976). However, O'Hara andStreet (1978) as well as Schü tze et al (1984) described callus formation from nodes but yielding infrequent shoot regeneration in wheat and oat.…”

Section: Discussionmentioning

confidence: 99%

Node-derived cultures with high-morphogenic competence in barley and wheat

Sharma

Hänsch

Mendel

et al. 2006

Plant Cell Tiss Organ Cult

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A successful regeneration system is presented for elite cultivars in barley and wheat based on nodes. Nodal explants were excised from in vitro and ex vitro grown plants. A combination of 8.28 lM 4-amino-3,5,6-trichloropicolinic acid and 4.54 lM to 22.71 lm thidiazuron (TDZ) used in MS-based medium containing 60 g l -1 maltose favoured induction of clumps of multiple shoots/buds with or without callus formation in the primary cultures. Within 8-10 weeks upon further subcultures, the proliferation into callus with rapid and continuously forming adventitious buds containing clusters of meristemoids, termed meristematic bulk tissue (MBT) was obtained. Lowering the levels of growth regulators resulted in redifferentiation of shoots, which elongated, rooted, developed into morphologically normal plants and set seeds normally. With a frequency ranging between 37 and 82% the nodes raised from in vitro grown plants were proliferated into MBT independent of TDZ concentration, cultivar and species. The average number of shoots per responding node in different cultivars was 7-15 in barley and 1-6 in wheat after 12-14 weeks. Nodes from greenhouse grown plants mainly responded for callus formation with poor development of MBT.

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In vitro Culture of Somatic Wheat Callus Tissue

Cited by 20 publications

References 0 publications

In Vitro Culture of Wheat Tissues. I. Callus Formation, Organ Redifferentiation and Single Cell Culture

In Vitro Culture of Wheat Tissues. I. Callus Formation, Organ Redifferentiation and Single Cell Culture

Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures

Node-derived cultures with high-morphogenic competence in barley and wheat

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