In Vitro Cytocompatibility of N-acetylcysteine–supplemented Dentin Bonding Agents

Kim, Na Ryoung; Park, Hee Chul; Kim, Insook; Lim, Bum-Soon; Yang, Hyeong‐Cheol

doi:10.1016/j.joen.2010.08.005

Cited by 22 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The supplementation of dentin bonding agents with N-acetylcysteine (NAC) was found to be useful to prevent the cytotoxicity 38) . Similar to this finding, NAC is shown to inhibit a decrease in the oxygen comsumption rate induced by HEMA 39) .…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

View full text Add to dashboard Cite

Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

show abstract

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

View full text Add to dashboard Cite

show abstract

“…For real‐time PCR, a 20‐μl reaction mixture containing 10 μl of SYBR Premix Ex Taq (Takara Bio, Otsu, Japan), 0.4 μl of ROX Reference Dye II (Takara Bio), cDNA, and primers were thermocycled in an ABI PRISM 7500 Sequence Detection System Thermal Cycler (Applied Biosystems, Foster City, CA, USA). The primer sequences and thermocycling conditions utilized in this experiment were as previously described . Expression of DSPP and OCN genes was evaluated on the basis of their threshold cycle ( C t ) values and was normalized to expression of the glyceraldehyde‐3‐phosphate dehydrogenase ( GAPDH ) gene.…”

Section: Methodsmentioning

confidence: 99%

Stimulating effects of quercetin and phenamil on differentiation of human dental pulp cells

Kim

Son

Park

et al. 2013

European J Oral Sciences

Self Cite

View full text Add to dashboard Cite

Dentin formation is preferred in the healing response of the pulp to pulp-capping agents during vital pulp therapy. Enhancement of the dentinogenic differentiation of dental pulp cells is thought to accelerate pulp repair. The aim of this study was to evaluate the dentinogenic activity of small molecules (three flavonoids and phenamil) that have been shown previously to induce osteoblast differentiation. Among the flavonoids (quercetin, genistein and baicalin), quercetin induced the highest alkaline phosphatase (ALP) activity of human dental pulp (HDP) cells. Phenamil, an amiloride derivative, elicited higher ALP activity than quercetin. However, increased expression of dentin sialophosphoprotein (DSPP) mRNA and mineral deposition were seen in cultures treated with quercetin compared with phenamil. This would seem to suggest that quercetin is the most dentinogenic agent among the tested chemicals. The increase in ALP activity in the quercetin-treated cells was not affected by ICI 182,780, an estrogen receptor inhibitor, and was partially blocked by PD98059, an extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitor. This suggests that ERK1/2 is activated in the quercetin-induced differentiation of HDP cells without the mediation of estrogen receptors, which are known to be involved in osteoblast differentiation induced by quercetin.

show abstract

“…ALP activity assay ALP activity was determined in HDPC that had been exposed to PSL for 3, 6 and 9 days in GM and DIM as described previously 12) . In brief, cells in 96-well plates were incubated in a mixture of 140 µL of alkaline buffer solution and 10 µL of 1.5 M MgCl 2 containing 67 mM 4-nitrophenyl phosphate (Fluka, Buchs, Switzerland) for 30 min at 37˚C.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells

Park

Quan

Yang

2017

Dental Materials Journal

Self Cite

View full text Add to dashboard Cite

Phosphatidylserine (PS) is known to enhance biomineralization due to the ability to accumulate calcium ions. In this study, the effects of PS on odontogenic differentiation and mineralization of human dental pulp cells (HDPCs) were investigated using phosphatidylserine-containing liposomes (PSLs). PSL was slightly cytotoxic at 125 µM in growth medium, and ALP activity was up-regulated in the PSL-treated HDPCs at non-cytotoxic concentrations. Mineralization was also enhanced by PSL, while mRNA expressions of DSPP and OCN genes were slightly attenuated. The mRNA expression of Runx2 was not altered by PSL. It is thus likely that PSL selectively affected odontogenic differentiation processes of HDPC. Finally, the interaction between PSL and HDPC was investigated by staining with annexin V-FITC in PSL-treated HDPC. It was found that PS was gradually incorporated into HDPC cytoplasm for several days. The results of this study suggest that PSL is able to stimulate dentin formation in dental pulps.

show abstract

In Vitro Cytocompatibility of N-acetylcysteine–supplemented Dentin Bonding Agents

Cited by 22 publications

References 31 publications

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Stimulating effects of quercetin and phenamil on differentiation of human dental pulp cells

Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells

Contact Info

Product

Resources

About