In vitro cytotoxic, genotoxic and oxidative stress of cypermethrin on five fish cell lines

Taju, G.; Majeed, Shahid; Nambi, K.S.N.; Farook, M.A.; Vimal, S.; Hameed, A.S. Sahul

doi:10.1016/j.pestbp.2014.06.006

Cited by 48 publications

(20 citation statements)

References 67 publications

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“…Caracterización de las aguas residuales de corrales, provenientes de una finca ganadera con manejo de ectoparásitos mediante bombeo por aspersión con cipermetrina Niveles de cipermetrina, como los mostrados en la tabla 1, pueden causar estrés oxidativo en especies como Clarias batrachus, Prochilodus lineatus y Cyprinus carpio y estar asociados con efectos citotóxicos en peces (Loteste et al 2013;Al-Ghanim 2014;Kumar et al 2014;Taju et al 2014). …”

Section: Resultados Y Discusiónunclassified

Remoción de cipermetrina presente en el baño de ganado utilizando humedales construidos

Marrugo‐Negrete

Ortega-Ruíz

Frómeta

et al. 2016

CTA

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<p>Ectoparasite control in the livestock sector involves the use of chemicals to prevent production losses. In small farms that produce milk in the Córdoba department, the use of the pumping system for the cattle bath is common between farmers. In this work, cypermethrin degradation efficiency was evaluated in three lab-scale subsurface flow constructed wetland planted with Limnocharis flava, Cyperus papyrus and Alpinia purpurata sp., and one unplanted system, all of the beds were gravel based; then, total suspended solids and total phosphorus retention, and elimination of chemical oxygen demand were measured as water quality parameters. The wastewater was pretreated in a descending-ascending slow sand filter, and then was conducted to a wetland continuous flow fed at 7 ml/min. Limnocharis flava bed was higher for the degradation of organic compounds, with 97.9 ± 2.5 % and 69.1 ± 3.7 % for cypermethrin and chemical oxygen demand respectively, with statistically significant differences (p < 0,05) respect to unplanted bed. The higher SST removal were found in the Cyperus papyrus wetland, with 62,0 %, however, no differences were observed with the other evaluated planted systems, as opposed these were significantly higher than unplanted wetlands.</p>

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Section: Resultados Y Discusiónunclassified

Remoción de cipermetrina presente en el baño de ganado utilizando humedales construidos

Marrugo‐Negrete

Ortega-Ruíz

Frómeta

et al. 2016

CTA

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“…To determine the effective concentration of H 2 O 2 , DrF cells were seeded at a density of 4,000 cells per well into 96well plates and grown for 24 hr and were exposed to various H 2 O 2 concentrations (0, 2, 4, 6, 8 and 10 µM, 6 wells per concentration) in a volume of 100 μl/well for 24 hr, after which the medium with H 2 O 2 was removed. The cell viability assessment was done using standard endpoint, that is, tetrazolium bromide MTT assay adopted following the procedure as mentioned in literature (Abdul Majeed et al, 2014;Mosmann, 1983;Taju, Abdul Majeed, Nambi, & Sahul Hameed, 2014). After 24 hr of exposure to different concentrations of H 2 O 2 , 20 µl MTT solution (5 mg/ml PBS) was added to each well.…”

Section: Cell Viability By Mtt Assaymentioning

confidence: 99%

Zebrafish fin‐derived fibroblast cell line: A model for in vitro wound healing

Sivalingam¹,

Seepoo²,

Taju³

et al. 2019

Journal of Fish Diseases

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The goal of this study was to develop and characterize a cell line from the caudal fin tissue of zebrafish and also its application as an in vitro model to study the effect of H2O2 in wound healing. Fibroblastic cell line was developed using explant culture method from caudal fin tissue of zebrafish and characterized. This cell line was named as DrF cell line. The DrF cells treated with 0–10 µM/ml H2O2 were tested for viability, proliferation and motility by MTT assay, trypan blue assay and chemotaxis assay, respectively. Among the different concentrations of H2O2, 4 µM was found to be nontoxic to study cell migration in in vitro scratch wound assay. Furthermore, the expression of proliferating cell nuclear antigen (PCNA) and chemokine receptor (CXCR4) genes was carried by qPCR. The cell survival, proliferation and migration were extremely enriched at 4 µM level of H2O2. We observed accelerated wound closure in DrF cells treated with H2O2. The qPCR results indicated that H2O2 markedly up‐regulated mRNA expression of PCNA and CXCR4. The findings from our study suggest that H2O2 at low levels promotes cell survival, proliferation, migration and wound healing in DrF cells.

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“…Piretroidler, dünyada 30 yılı aşkın bir süredir tarımda, halk sağlığı için evlerde ve küçükbaş hayvanlarda ektoparazitlere karşı kullanılmaktadır (Soderlund et al 2002;Piner, 2009). İnsanlarda sentetik piretroidleremaruziyete bağlı olarak fenvalerent ve deltametrin ile ilgili Çin'de akut zehirlenme olayları kaydedilmiştir (Çakır, 2008 (Taju et al 2014;Song et al 2015;Vardavas et al 2016;Yun et al 2017 …”

Section: Tartışma Ve Sonuçunclassified

Evaluation of Siperkor Pesticide Mutagenicity with Ames Test

Özkara¹

2017

AKU-J. Sci. Eng.

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ÖzetPestisitler tarım sektöründe ürünlerin verimini, kalitesini artırmak ve ürün depolanması için yaygın olarak kullanılmaktadır. Ancak pestisitler hedef olmayan organizmalara zarar vermeleri, pestisit direncine sebep olmaları, bazılarının damutajen ve karsinojen olmasından dolayı endişe yaratmaktadırlar. Bu çalışmada tarımda yaygın olarak kullanılan piretroid grubu bir insektisit olan Siperkor'un mutajenitesi Ames testi ile değerlendirilmiştir. Ames testinde Siperkor'un 5 farklı dozu (250, 25, 2.5, 0.25, 0.025 µg/plak) TA98 veTA100 suşları kullanılarak hem S9 karaciğer enzimi fraksiyonu varlığında hem de yokluğunda test edilmiştir. Her test suşu için deney sırasında suşa özel bir pozitif kontrol kullanılmıştır. TA98 suşu S9'lu deney için 2-aminofluorene (AF), S9'suz deney için 4-nitro-ophenylenediamine(NPD) kullanılırken, TA100 suşu için S9'lu deneyde 2-aminoanthracene (2AA), S9'suz deney için ise sodyum azide (SA)pozitif kontrol olarak kullanılmıştır. Ames testi sonuçlarına göre Siperkor pestisiti sadece TA98 suşunda 250 µg/plak konsantrasyonunda hem S9 fraksiyonu varlığında hem de yokluğunda mutajenik aktivite göstermiştir. Evaluation of Siperkor Pesticide Mutagenicity with Ames TestKeywords Ames Test;Mutagenicity; Siperkor;Pesticide AbstractPesticides are widely used in agriculture to improve the efficiency and quality of products and food storage. However there is a big concern about their usage because some of them are mutagens and/or carcinogens, harm non-target organisms or cause pest resistance. In this study Siperkor insectidepyretroid group-which is widely used in agriculture was determined for mutagenicity by using Ames test. Five different concentrations (250, 25, 2.5, 0.25, 0.025 µg/plate), of Siperkor pesticide was tested by Ames test on TA98 and TA100 strains with and without S9 metabolic activation. For each tester strain, a specific positive control was always used to test the experimental flaws, if any. While 4-nitro-ophenylenediamine (NPD) for TA98, sodium azide (SA) for TA100, was used as positive controls without metabolic activation, 2-aminofluorene (AF), 2-aminoanthracene(2AA) was used as positive controls with metabolic activation, respectively. The positive controls also were used in each tester strains. According to Ames test results, only 250 µg/plate, concentration of Siperkor pesticide has shown mutagenic activity on TA98 strain with and without S9 fraction.

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In vitro cytotoxic, genotoxic and oxidative stress of cypermethrin on five fish cell lines

Cited by 48 publications

References 67 publications

Remoción de cipermetrina presente en el baño de ganado utilizando humedales construidos

Remoción de cipermetrina presente en el baño de ganado utilizando humedales construidos

Zebrafish fin‐derived fibroblast cell line: A model for in vitro wound healing

Evaluation of Siperkor Pesticide Mutagenicity with Ames Test

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