In vitro Cytotoxicity and Glucose Uptake Activity of Fruits of Terminalia bellirica in Vero, L-6 and 3T3 cell lines

Das, Mary Shoba; Devi, G. Karthiga

doi:10.7324/japs.2015.501215

Cited by 22 publications

(14 citation statements)

References 9 publications

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“…The cytotoxic effect of the most active phytoconstituent (diterpenes) of Berberis aristata root bark was studied against three cell lines [RD (Human Rhabdomyosarcoma), L20B (Diploid mouse lung cell line) and Hep 2 (Human larynx epidermoid carcinoma)] by MTT assay as described earlier in (Al-Asady et al 2014; Das and Devi 2015) with slight modifications, as per protocol given in Additional file 2. The cell lines were procured from Central Research Institute (C.R.I), Kasauli, Himachal Pradesh, India.…”

Section: Methodsmentioning

confidence: 99%

Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

et al. 2019

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Berberis aristata is an important part of traditional healing system from more than 2500 years. The aqueous extract of Berberis aristata root bark displayed broad spectrum activity against 13 test pathogens, ranging from 12 to 25 mm. In classical optimization, 15% concentration prepared at 40 °C for 40 min was optimal and thermostable. Statistical optimization enhanced the activity by 1.13–1.30-folds. Ethyl acetate was the best organic solvent to elute out the potential compound responsible for antimicrobial activity. Diterpenes were the most abundant phytoconstituent (15.3%) and showed broad spectrum antimicrobial activity ranging from 16.66 to 42.66 mm. Ethyl acetate extract displayed the lowest minimum inhibitory concentration (0.05–1 mg/mL), followed by diterpenes (0.05–5 mg/mL) and flavonoids (0.05–10 mg/mL). The test extracts were microbicidal in nature and showed a prolonged post antibiotic effect ranging from 2 to 8 h. They were found to be biosafe as per Ames and MTT assay. The in vitro cytotoxicity evaluation of diterpenes against L20B, RD and Hep 2 cell lines revealed its IC50 ranging from 245 to 473 µg/mL. Acute oral toxicity of diterpenes on Swiss albino mice did not show any changes in behavioral pattern, body weight, biochemical parameters as well as organs’ architecture. The study thus indicates B. aristata could be a potential candidate for development of potent drug owing to its antimicrobial potential and biosafe profile.

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Section: Methodsmentioning

confidence: 99%

Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

et al. 2019

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“…It was also observed that the crude ethanol extract of Syzygium jambolanum seed increased the glucose uptake by yeast cells in vitro at lower concentration 36 . The L6 cell lines derived from skeletal muscle serves as a good model for glucose uptake study 37 .…”

Section: Discussionmentioning

confidence: 99%

An in Vitro Study of Syzygium Cumini Seed Extract on Glucose Uptake Activity in L-6 Cell Lines

Maneemegalai¹,

Narmatha²

2019

J. Drug Delivery Ther.

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Diabetes mellitus is the most common endocrine disorder. The plant Syzygium cumini has been used in traditional medicine for the treatment of diabetes. The present study investigated the effect of ethanol extract of S. cumini seeds on uptake of glucose by L-6 rat skeletal muscle cells. S. cumini seeds were extracted with varying solvents and quantitative phytochemical analysis was carried out, ethanol extract of seeds exhibited higher content of tested phytochemicals. The effect of different concentrations (300µg/ml – 1000µg/ml) of ethanol extract of seeds were studied on glucose uptake activity of L-6 rat skeletal muscle cells. It was observed that with the increase in concentration, the glucose uptake activity was also increased. The results of the study supports and demonstrates the antidiabetic potential of ethanol seed extracts of Syzygium cumini utilizing in vitro model. KEY WORDS: Diabetes mellitus, Syzygium cumini, phytochemicals, glucose uptake, L-6 cells

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“…The flavonoids was studied for their activity against the three cell lines obtained from Central Research Institute (C.R.I), Kasauli (H.P. ), India by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay as described previously [ 33 – 35 ] with slight modifications. The cytotoxic effect was compared to a normal cell line , i.e., Vero (African Green monkey kidney normal cell) line.…”

Section: Methodsmentioning

confidence: 99%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

BMC Pharmacol Toxicol

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Background Plants provide a ray of hope to combat the ever increasing antibiotic resistance and Symplocos racemosa is a valuable medicinal plant. The study focused on highlighting the importance of this plant’s phytoconstituents as potential source of novel antimicrobials against planktonic as well as biofilm forming microorganisms, along with their antiproliferative activity. The biosafety of the phytoconstituents was also established, followed by detection of probable antimicrobial components. Methods The best organic extractant and major groups of phytoconstituents were tested for their antimicrobial activity against reference microbial strains and drug-resistant clinical isolates. The anti-proliferative potential of the most active group of phytoconstituents was evaluated against cancerous cell lines. The in vitro biosafety of phytoconstituents was evaluated by Ames and MTT assay, while in vivo biosafety of the most active phytoconstituents, i.e., flavonoids was determined by acute oral toxicity. Further, the probable antimicrobial components in the flavonoids were detected by TLC and GC-MS. Results Ethyl acetate extract was the most effective among various organic extracts, whereas phytoconstituents such as flavonoids, cardiac glycosides, saponins, tannins, triterpenes and phytosterols were the major groups present, with flavonoids being the most potent antimicrobials. The phytoconstituents displayed a significant antibiofilm potential, as exhibited by inhibition of initial cell attachment, disruption of the pre-formed biofilms and reduced metabolic activity of biofilms. The phytoconstituents were significantly active against the drug-resistant strains of E.coli, MRSA and Salmonella spp. Further, flavonoids showed significant cytotoxic effect against the cancerous cell lines but were non-cytotoxic against Vero (normal) cell line. All the test preparations were biosafe, as depicted by the Ames test and MTT assay. Also, flavonoids did not induce any abnormality in body weight, clinical signs, biochemical parameters and organs’ histopathology of the Swiss albino mice during in vivo acute oral toxicity studies. The flavonoids were resolved into 4 bands (S1-S4), where S3 was the most active and its GC-MS analysis revealed the presence of a number of compounds, where Bicyclo [2.2.1]heptan-2-one,1,7,7-trimethyl-, (1S)- was the most abundant. Conclusions These findings suggest that the phytoconstituents from Symplocos racemosa bark could act as potential source of antimicrobial as well as antiproliferative metabolites.

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In vitro Cytotoxicity and Glucose Uptake Activity of Fruits of Terminalia bellirica in Vero, L-6 and 3T3 cell lines

Cited by 22 publications

References 9 publications

Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

An in Vitro Study of Syzygium Cumini Seed Extract on Glucose Uptake Activity in L-6 Cell Lines

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

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