In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

Shaheen, Fozia; Aziz, Muhammad Hammad; Fatima, Mahvish; Khan, M. Ajmal; Ahmed, Farhana; Ahmad, Riaz; Ahmad, Muhammad; Alkhuraiji, Turki S.; Akram, Muhammad Waseem; Raza, Rizwan; Ali, Syed Mansoor

doi:10.3390/nano8070539

Cited by 33 publications

(14 citation statements)

References 64 publications

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“…Moreover, the highest LDH release was in CuO NPs (100 mg/ml) with R þ H groups as compared to control. Shaheen et al revealed that assessments of the GO-ZnO against the MCF-7 Cells, a significant increased the LDH release in a dose-dependent manner [40].…”

Section: Discussionmentioning

confidence: 99%

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Ghaleh

Zarei

Motlagh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of this paper was examining the combined impacts of CuO nanoparticles (CuO NPs), hyperthermia (H), and irradiation (R) on an increment of MCF-7 cells. The MTT assay was employed to assess the antiproliferative effects of CuO NPs (25, 50, and 100 lg/ml), hyperthermia (41 C for 1 h), and irradiation (200 cGy). Moreover, the perniciousness was estimated through the survival capability of cells, and apoptosis, ROS production, and levels of caspase-3,-8 and-9 proteins were determined. A significant (p < .01) decrease in proliferation index (0.124 ± 0.021), a significant (p < .01) increase in apoptosis (42% ± 1.54) of MCF7 cells, a significant (p < .03) increase in ROS formation (32.16 ± 1.9) and a significant (p < .01) increase in LDH release (33.28 ± 1.56) were recorded in the adjacency of MCF-7 cells by a combination of CuO NPs (100 mg/ml) and R þ H compared to control and other treatments. The activities of caspase-3 (0.33 ± 0.014) and caspase-9 (0.389 ± 0.019) also increased significantly (p < .05). However, caspase-8 showed no significant changes in its activity (p ¼ .065). Based on these observations, a combination of CuO NPs, hyperthermia, and irradiation could suppress the growth of MCF-7 cells and evoke cell apoptosis via mitochondrial membrane potential.

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Section: Discussionmentioning

confidence: 99%

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Ghaleh

Zarei

Motlagh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…•− ), hydroxyl radical (HO•), and hydrogen peroxide (H 2 O 2 ) serve as signaling molecules in the process of apoptosis [46]. The GO-ZnO nanocomposite was found to induce oxidative stress mediated toxicity in MCF-7 cells [47]. In this study, we examined several parameters of oxidative stress in A549 cells against CeO 2 NPs, RGO, and CeO 2 -RGO nanocomposite when exposed to said particles for 24 h. The quantitative data demonstrated that the intracellular level of ROS in the CeO 2 NP group was almost similar to that of the control group, but significantly higher than the RGO group ( Figure 8A).…”

Section: Oxidative Stressmentioning

confidence: 99%

Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

et al. 2019

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Graphene-based nanocomposites have attracted enormous interest in nanomedicine and environmental remediation, owing to their unique characteristics. The increased production and widespread application of these nanocomposites might raise concern about their adverse health effects. In this study, for the first time, we examine the cytotoxicity and oxidative stress response of a relatively new nanocomposite of cerium oxide-reduced graphene oxide (CeO2-RGO) in human lung epithelial (A549) cells. CeO2-RGO nanocomposites and RGO were prepared by a simple hydrothermal method and characterized by relevant analytical techniques. Cytotoxicity data have shown that RGO significantly induces toxicity in A549 cells, evident by cell viability reduction, membrane damage, cell cycle arrest, and mitochondrial membrane potential loss. However, CeO2-RGO nanocomposites did not cause statistically significant toxicity as compared to a control. We further observed that RGO significantly induces reactive oxygen species generation and reduces glutathione levels. However, CeO2-RGO nanocomposites did not induce oxidative stress in A549 cells. Interestingly, we observed that CeO2 nanoparticles (NPs) alone significantly increase glutathione (GSH) levels in A549 cells as compared to a control. The GSH replenishing potential of CeO2 nanoparticles could be one of the possible reasons for the biocompatible nature of CeO2-RGO nanocomposites. Our data warrant further and more advanced research to explore the biocompatibility/safety mechanisms of CeO2-RGO nanocomposites in different cell lines and animal models.

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“…The cellular morphology of HUH-7 and WRL-68 cells were thoroughly studied, after treatment, under the phase-contrast microscope, and images were taken by Olympus camera for further comparison. In published studies, distortion of cellular morphology along with membrane disintegration, changed in shape and size is an indication of molecular responses because of nanoparticles [91]. Hence, we verified and compared the prominent effect of IONP with 5FU and hygromycin B on the cellular morphology.…”

Section: Mechanism Of Photocatalytic Degradationmentioning

confidence: 57%

Green Synthesis of Iron Oxide Nanoparticles to Explore Cytotoxic Behaviour and to Diminish Environmental Pollution by a Novel Contrivance

2021

JCBSC

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To overcome the side effects of conventional chemotherapy, the study of effective anticancer application of magnetic iron oxide nanoparticles is now-a-days a great area of research which is increasing day by day. In our present study, Iron oxide nanoparticles (IONPs) have been synthesised by reducing ferrous sulphate using Punica granatum peel extracts. Punica granatum peel extract has a rich source of flavonoids and phenolic compounds with high antioxidant and these molecules can be used as excellent reducing agents. The nanopowders have been calcined at 700 0 C for 4 hours. Physicochemical, optical, photocatalytic, cytotoxicity activities (both in normal and carcinoma cell) and morphological assessment of carcinoma cells are characterised using analytical techniques. XRD data, DLS, FTIR spectra and Scanning electron microscope analysis have confirmed the formation of IONPs having spherical shape with trigonal (rhombohedral axes) phase structure, smaller size (average size from XRD 43-47 nm) and large surface area. From UV-Vis absorption spectra and Tauc plot the optical band gap has been obtained 3.5 e.V. IONPs show good photo-Fenton in Methylene blue (MB) dye degradation within 15 minutes with ~91% and 40 minutes with 99% for under optimum condition. The cytotoxicity in Human hepatic cell line (WRL-68) and human hepatocellular carcinoma (HUH-7) cell lines have also been observed using MTT assay and verified Green Synthesis … Shatarupa Basak et al.

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In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

Cited by 33 publications

References 64 publications

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

Green Synthesis of Iron Oxide Nanoparticles to Explore Cytotoxic Behaviour and to Diminish Environmental Pollution by a Novel Contrivance

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