Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Ghaleh, Hadi Esmaeili Govarchin; Zarei, Leila; Motlagh, Bahman Mansori; Jabbari, Nasrollah

doi:10.1080/21691401.2019.1600529

Cited by 32 publications

(17 citation statements)

References 43 publications

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“…It has been reported that HSP, which confers thermotolerance to cells, can also be expressed by mechanical stimulation rather than heat shock 25 . Alternatively, it was recently reported that combinations of hyperthermia and other stimuli enhanced the cell killing effect 10 , 15 , 26 , 27 . On the other hand, in previous studies, owing to the lack of a system that achieves immediate temperature regulation, there was a gap between the set temperature and the actual realized culture temperature to which cells were exposed.…”

Section: Discussionmentioning

confidence: 99%

Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells

Imashiro

Takeshita

Morikura

et al. 2021

Sci Rep

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Hyperthermia has been studied as a noninvasive cancer treatment. Cancer cells show stronger thermal cytotoxicity than normal cells, which is exploited in hyperthermia. However, the absence of methods evaluating the thermal cytotoxicity in cells prevents the development of hyperthermia. To investigate the thermal cytotoxicity, culture temperature should be regulated. We, thus, developed a culture system regulating culture temperature immediately and accurately by employing metallic culture vessels. Michigan Cancer Foundation-7 cells and normal human dermal fibroblasts were used for models of cancer and normal cells. The findings showed cancer cells showed stronger thermal cytotoxicity than normal cells, which is quantitatively different from previous reports. This difference might be due to regulated culture temperature. The thermal stimulus condition (43 °C/30 min) was, further, focused for assays. The mRNA expression involving apoptosis changed dramatically in cancer cells, indicating the strong apoptotic trend. In contrast, the mRNA expression of heat shock protein (HSP) of normal cells upon the thermal stimulus was stronger than cancer cells. Furthermore, exclusively in normal cells, HSP localization to nucleus was confirmed. These movement of HSP confer thermotolerance to cells, which is consistent with the different thermal cytotoxicity between cancer and normal cells. In summary, our developed system can be used to develop hyperthermia treatment.

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Section: Discussionmentioning

confidence: 99%

Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells

Imashiro

Takeshita

Morikura

et al. 2021

Sci Rep

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show abstract

“…Absorption at 570 nm was measured by Elisa reader (BioTek Instrument, USA). The half maximal inhibitory concentration (IC50) was determined by linear regression based on Equation ( 4) [25]. In order to calculate the cell viability, Equation (5) was used as follows: [26]…”

Section: In Vitro Cytotoxicity Studiesmentioning

confidence: 99%

Preparation, characterization and in vitro evaluation of PEGylated nanoliposomal containing etoposide on lung cancer

Kazemabadi

Heydarinasab

Akbarzadeh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Introduction and objective: Lung cancer is the most common one in terms of outbreak and mortality. Since most modern treatments have many side effects, finding an effective and alternative therapy seems necessary. The present study aimed to determine the effect of PEGylated liposomal etoposide nanoparticles on the lung cancer (A-549 and Calu6 cell lines). Materials and methods: The PEGylated liposomal etoposide nanoparticles were prepared by reversephase evaporation method. The particle size and zeta potential of the nanoparticles were measured by Zetasizer. The nanoparticle cytotoxicity was examined by MTT method. The vesicular drug release pattern was examined by dialysis method. The amount of loaded drug and the encapsulation efficiency (EE) was also measured and calculated. Apoptosis test was performed using flow cytometry with Annexin V kit. Results: The mean particle size, size distribution, and zeta potential of PEGylated liposomal etoposide nanoparticles were 122.5 ± 4.8 nm, 0.252 ± 0.12 and À13.7 ± 0.51 mv, respectively. The etoposide release in prepared formulations was detected to be about 15.64% after 50 hr. The cytotoxic effect of etoposide nanoparticles on lung cancer A-549 and Calu6 cell lines showed more anti-tumour activity compared to the free drug used. Conclusion:The results showed that the PEGylated liposomal nanoparticles were used as a suitable nanocarrier for etoposide injection. It was also found that the drug effect on the nanodrug formulations was higher than that of the free drug.

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“…Breast cancer is the second driving reason for cancer-based mortality among women and the most generally identified cancer after skin-related cancers [3]. Radiation therapy, so called radiotherapy, is among the most important methods of cancer treatment, which applies ionizing radiation (IR) to eradicate cancerous cells [4]. The radiotherapy not only is considered as primary therapy, but also is used along with chemotherapy, hormone therapy, and surgery [5,6].…”

Section: Introductionmentioning

confidence: 99%

Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy

Jabbari

Nawaz

Rezaie

2019

IJMS

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Radiation therapy, which applies high-energy rays, to eradicate tumor cells, is considered an essential therapy for the patients with breast cancer. Most tumor cells secrete exosomes, which are involved in cell-to-cell communication in tumor tissue and contribute therapeutic resistance and promote tumor aggressiveness. Here, we investigated the effect of clinically applicable doses of X-ray irradiation (2, 4, 6, 8, 10 Gy) on the dynamics of the exosomes’ activity in MCF-7 breast cancer cells. Survival and apoptosis rate of cells against X-ray doses was examined using MTT and flow cytometry assays, respectively. Whereas, the levels of reactive oxygen species (ROS) in the X-ray-treated cells were detected by fluorometric method. The mRNA levels of vital genes involved in exosome biogenesis and secretion including Alix, Rab11, Rab27a, Rab27b, TSPA8, and CD63 were measured by real-time PCR. The protein level of CD63 was examined by Western blotting. Additionally, exosomes were characterized by monitoring acetylcholinesterase activity, transmission electron microscopy, size determination, and zeta potential. The result showed that in comparison with control group cell survival and the percentage of apoptotic cells as well as amount of ROS dose-dependently decreased and increased in irradiated cells respectively (p < 0.05). The expression level of genes including Alix, Rab27a, Rab27b, TSPA8, and CD63 as well as the protein level of CD63 upraised according to an increase in X-ray dose (p < 0.05). We found that concurrent with an increasing dose of X-ray, the acetylcholinesterase activity, size, and zeta-potential values of exosomes from irradiated cells increased (p < 0.05). Data suggest X-ray could activate exosome biogenesis and secretion in MCF-7 cells in a dose-dependent way, suggesting the therapeutic response of cells via ROS and exosome activity.

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Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Cited by 32 publications

References 43 publications

Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells

Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells

Preparation, characterization and in vitro evaluation of PEGylated nanoliposomal containing etoposide on lung cancer

Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy

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