In vitro cytotoxicity of one-step dentin bonding systems

Hashieh, Imad Abou; Cosset, Anne; Franquin, Jean‐Claude; Camps, Jean

doi:10.1016/s0099-2399(99)80003-x

Cited by 35 publications

(24 citation statements)

References 12 publications

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“…In the present study, the cytotoxicity of four different one-step, self-etching dentin bonding agents were analyzed with MTT assay, DCF assay, 8-OHdG measurement and comet assay in L929 mouse fibroblast cell culture model. The cytotoxicity of the resin based materials are related with dilution rate 3,27,28) . The tested dentin bonding agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect on L929 cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

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Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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“…The factors that inhibit dehydrogenase activity affect the associated color reaction. It has been shown that activated cells produce more formazan than resting cells; therefore, it is possible to measure cell activity or enzyme activities [23][24][25][26][27][28] . With direct contact tests, all primers/conditioner/ adhesives showed cytotoxicity to L-929 cells and 50% and 20% diluted solutions were found to be more toxic than the other dilutions for all materials.…”

Section: Discussionmentioning

confidence: 99%

“…With direct contact tests, all primers/conditioner/ adhesives showed cytotoxicity to L-929 cells and 50% and 20% diluted solutions were found to be more toxic than the other dilutions for all materials. Hashieh et al 24) investigated the cytotoxicity of 10 −1 -10 −8 diluted fourth and fifth generation of bonding systems on L-929 cells with MTT test and found that the level of cytotoxic effects was reduced with the dilution rate.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

et al. 2011

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This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfil Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

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“…Each experiment was performed 6 times and the cell cytotoxicity was calculated according to the following equation [19]:…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of the cytotoxicity of FotoSan™ light-activated disinfection on human fibroblasts

et al. 2011

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SummaryBackgroundRoot canal disinfection needs to be improved because actual techniques are not able to eliminate all microorganisms present in the root canal system. The aim of the present study was to investigate the in vitro cytotoxicity of FotoSan (CMS Dental APS, Copenhagen Denmark), 17% EDTA and 2% chlorhexidine.Material/MethodsFibroblasts of periodontal ligament from healthy patients were cultured. FotoSan (with and without light activation for 30 sec.), 17% EDTA and 2% chlorexidine were used for the cell viability tests. Untreated cells were used as control. The cellular vitality was evaluated by MTT test. The production of reactive oxygen species (ROS) was measured using an oxidation-sensitive fluorescent probe. Results were statistically analyzed by ANOVA, followed by a multiple comparison of means by Student-Newman-Keuls, and the statistical significance was set at p<0.05.ResultsMTT tests showed that cytotoxic effects of FotoSan (both photocured and uncured) were statistically lower (p<0.05) than that observed using 2% Chlorhexidine, while no significant differences were found in comparison with 17% EDTA. No alterations in ROS production were detectable in any of the tested materials.ConclusionsSince the toxicity of the FotoSan photosensitizer, both light-activated and not light-activated, is similar to common endodontic irrigants, it can be clinically used with precautions of use similar to those usually recommended for the above-mentioned irrigating solutions.

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In vitro cytotoxicity of one-step dentin bonding systems

Cited by 35 publications

References 12 publications

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

In vitro evaluation of the cytotoxicity of FotoSan™ light-activated disinfection on human fibroblasts

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