In vitro cytotoxicity studies of palladacyclic complexes containing the symmetric diphosphine bridging ligand. Studies of their interactions with DNA and BSA

Karami, Kazem; Hosseini-Kharat, Mahboubeh; Sadeghi‐Aliabadi, Hojjat; Lipkowski, Janusz; Mirian, Mina

doi:10.1016/j.ejmech.2013.11.042

Cited by 72 publications

(24 citation statements)

References 74 publications

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“…Similarly, considering Fig. 4 and previous reports (17,18,30,(31)(32)(33)(34)(35)(36), it is conceivable that the components to which the Pd compound molecules (negatively charged in neutral pH) could be chelated and/or adsorbed are CW_TMP, positively charged DBP in neutral pH, and DNA bases in E. coli cells. In contrast, as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Pasteurized milk contains granulocytes, many genera of dead bacteria, lipids, proteins, and sugars; in brief, pasteurized milk contains a number of PCR inhibitors (26). Milk proteins, enzymes, and nucleic acids inside or outside cow cells are also likely to disturb the function of Pd compounds (12)(13)(14)(15)(16)(17)(18).…”

Section: Discussionmentioning

confidence: 99%

“…We were also very interested in the interaction between palladium compounds and bovine serum albumin (BSA) in view of the effects of Pd compounds on proteins and enzymes (18). Thus, we evaluated whether treatment with Pd compounds followed by PCR could improve LD discrimination, and then we elucidated the reaction mechanism of Pd compounds for LD discrimination.…”

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confidence: 99%

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Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR

Soejima

Iwatsuki

2016

Appl Environ Microbiol

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Ethidium monoazide and propidium monoazide (EMA and PMA) have been used in combination with PCR for more than a decade to facilitate the discrimination of live and dead bacteria (LD discrimination). These methods, however, require many laborious procedures, including the use of a darkroom. Here, we demonstrate an innovative use of palladium compounds involving lower limits of detection and quantification of targeted live cells, fewer laborious procedures, lower costs, and potentially higherthroughput analysis than the use of EMA and PMA. We have also recently reported platinum compounds for LD discrimination, but platinum compounds carry costs that are 3 times higher because of the requirement for much larger amounts for LD discrimination than palladium compounds. Palladium compounds can penetrate dead (compromised) but not live bacteria and can be chelated primarily by chromosomal DNA and cell wall transmembrane proteins, with small amounts of DNA-binding proteins in vivo. The new mechanism for palladium compounds is obviously different from that of platinum compounds, which primarily target DNA. Combining palladium compounds with PCR (Pd-PCR) in water resulted in discrimination between live and dead Enterobacteriaceae bacteria that was much clearer than that seen with the PMA method. Pd-PCR correlated with reference plating or with the currently used PMA-PCR method for pasteurized milk, based on EN ISO 16140:2003 validation. Pd-PCR enabled us to specifically detect and assay viable Enterobacteriaceae cells at concentrations of 5 to 10 CFU/ml in milk while following U.S./EU regulations after a 4.5-h process in a typical laboratory exposed to natural or electric light, as specified by U.S./EU regulations. IMPORTANCEEthidium monoazide and propidium monoazide (EMA and PMA) facilitate the discrimination of live and dead bacteria (LD discrimination). These methods, however, require many laborious procedures, including the use of a darkroom. Here, we demonstrate an innovative use of palladium compounds involving fewer laborious procedures, lower costs, and potentially higherthroughput analysis than the use of EMA and PMA. We have also recently reported platinum compounds for LD discrimination, but platinum compounds carry costs that are 3 times higher because of the requirement for much larger amounts for LD discrimination than palladium compounds, which have also a novel reaction mechanism different from that of platinum compounds. In view of testing cost, palladium compounds are also very useful here compared with platinum compounds. Ultimately, the innovative Pd-PCR method may be also substituted for the currently used reference plating methods. The PCR is a widely available tool that is used to detect bacteria and viruses in food and in environmental and clinical samples. However, PCR cannot distinguish live from dead bacteria. During reverse transcription-PCR targeting mRNA, a high concentration of contaminating dead bacteria (4 log 10 to 7 log 10 cells/ml) will trigger a false-positive result owing to t...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR

Soejima

Iwatsuki

2016

Appl Environ Microbiol

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“…The formation of 1 was readily confirmed by a characteristic stretching vibration band at 2052 cm À1 assigned to the bridged N 3 group [37]. 31 In the FT-IR spectra of 1a and 1b, a strong stretching vibrations were observed at 2026 and 2032 cm À1 due to the terminal azide group, respectively, which were shifted to lower wavenumber compared to the bridging azide group present in the starting material (2052 cm À1 ) [40]. Moreover, in the IR spectra of 1a and 1b, PAC (C 6 H 5 ) bond stretching vibrations were observed in the 1106 and 1103 cm À1 , respectively.…”

Section: Synthesis and Spectroscopic Characterizationmentioning

confidence: 96%

“…Many studies dealing with the interactions between some small molecules and BSA have been carried out because the tertiary structure of BSA is similar to HSA in more than 76% of its overall structure [22]. Compared to the number of organic molecules [23][24][25][26], relatively few metal complexes [27][28][29] DNA/BSA binding affinity [30,31]. However, the studies on the DNA and protein binding interaction of palladium complexes containing the C,C-chelating phosphorus ylide have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

Karami

Shirani-Sarmazeh

Hosseini-Kharat

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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New heteroleptic palladium(II) dithiocarbamates: synthesis, characterization, packing and anticancer activity against five different cancer cell lines

Khan

Amir

Ullah

et al. 2016

Applied Organom Chemis

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Two new heteroleptic palladium(II) complexes have been synthesized by reacting equimolar quantities of palladium(II) chloride, sodium 4‐(2‐methoxyphenyl)piperazine‐1‐carbodithioate and diphenyl‐p‐tolylphosphine (1) or tri‐p‐tolylphosphine (2). Complexes 1 and 2 have been characterized using elemental analysis, Fourier transform infrared spectroscopy, multinuclear NMR (1H, 13C and 31P) spectroscopy and single‐crystal X‐ray analysis. The latter technique confirms a pseudo square‐planar geometry in which two adjacent positions are occupied by bidentate dithiocarbamate while chloro and substituted triphenylphosphine are present at the remaining two positions. The anticancer activity of both complexes against five different cancer cell lines (LU – human lung carcinoma, established at UIC, Department of Surgical Oncology; MCF7 – human breast adenocarcinoma, ATCC number HTB‐22™; MDA‐MB‐231 – human breast adenocarcinoma, ATCC number HTB‐26™; Hepa‐1c1c7 – mouse liver hepatoma, ATCC number CRL‐2026™; PC‐3 – human prostate adenocarcinoma, ATCC number CRL‐1435™) was determined by MTT assay, revealing 2 has higher activity than 1. A drug–calf thymus DNA binding study with UV–visible spectroscopy reveals a higher DNA binding affinity of 2 (3.511 × 104 M−1) than 1 (4.213 × 103 M−1). Density functional theory studies confirm the relatively more stable nature of 2 than 1. Copyright © 2016 John Wiley & Sons, Ltd.

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In vitro cytotoxicity studies of palladacyclic complexes containing the symmetric diphosphine bridging ligand. Studies of their interactions with DNA and BSA

Cited by 72 publications

References 74 publications

Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR

Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR

Synthesis, spectral characterization, crystal structure and in vitro DNA/protein binding studies of phosphorous ylide palladacyclic complexes containing azide group

New heteroleptic palladium(II) dithiocarbamates: synthesis, characterization, packing and anticancer activity against five different cancer cell lines

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