In vitro decidualization of human endometrial stromal cells

Tabanelli, S; Tang, Baiqing; Gurpide, Erlio

doi:10.1016/0960-0760(92)90137-8

Cited by 134 publications

(76 citation statements)

References 22 publications

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“…Small heat shock proteins have been detected in rat and mouse uterine tissue during implantation where they are thought to play a role in decidualization (Tabanelli et al 1992, Bany & Schultz 2001. In addition, Hsp27 has been examined in human endometrial stromal cells during in vivo and in vitro decidualization (Tang et al 1993, Ciocca et al 1996, Shah et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour

White¹,

Williams²,

Highmore³

et al. 2005

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The underlying mechanisms that regulate uterine contractions during labour are still poorly understood. A candidate regulatory protein is heat shock protein 27 (Hsp27). It belongs to the small heat shock protein family and can regulate actin cytoskeleton dynamics, act as a chaperone, and may regulate contractile protein activation. As a result, we hypothesized that Hsp27 expression would be highly induced during late pregnancy and labour. Hsp27 mRNA expression was significantly elevated (P < 0.05) on days 17 to 22 of gestation. In addition, immunoblot analysis demonstrated that detection of total Hsp27 increased (P < 0.05) between day 21 and 1 day post-partum (PP) inclusive. Since phosphorylation of Hsp27 has been reported to be a prerequisite for smooth muscle contraction, we examined the temporal and spatial expression of Ser-15 phosphorylated Hsp27. Immunoblot analysis showed that the detection of Ser-15 phosphorylated Hsp27 significantly increased (P < 0.05) between days 19 and 23 (active labour) inclusive, in parallel with detection of total Hsp27. Immunocytochemical analysis of Ser-15 phosphorylated Hsp27 expression in situ demonstrated that phosphorylated Hsp27 in circular muscle became detectable in peri-nuclear and membrane regions on days 19 to 22, but was primarily restricted to the cytoplasm on days 23 to PP. In contrast, phosphorylated Hsp27 in longitudinal muscle was primarily detected in myocyte membranes on days 15 to 22, and then also became detectable in the cytoplasm of myocytes on days 23 and PP. Our results demonstrate that Hsp27 expression is highly upregulated during late pregnancy and labour and suggest that Hsp27 is a potential candidate contraction-associated protein.

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Section: Introductionmentioning

confidence: 99%

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour

White¹,

Williams²,

Highmore³

et al. 2005

Reproduction

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“…1). The decidualized endometrial stromal cell (ESC) becomes rounded, acquires myofibroblast characteristics, and secretes a variety of phenotypic antigens, including prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1) and tissue factor (TF) (Daly et al 1983, Irwin et al 1989, Tabanelli et al 1992, Tseng et al 1992, Lockwood et al 1993, Gellersen et al 1994, Oliver et al 1999b, Christian et al 2001b. At a molecular level, decidual transformation involves extensive reprogramming of many cell functions including altered steroid hormone receptor expression and steroid metabolism, remodelling of the extracellular matrix and cytoskeleton, altered expression of intracellular enzymes, growth factors and cytokines and their receptors, and induction of apoptosis modulators and decidua-specific transcription factors (Oliver et al 1999a, Popovici et al 2000, Brar et al 2001.…”

Section: Introductionmentioning

confidence: 99%

Cyclic AMP and progesterone receptor cross-talk in human endometrium: a decidualizing affair

Gellersen¹,

Brosens²

2003

Journal of Endocrinology

401

378

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During the menstrual cycle, the ovarian hormones oestradiol and progesterone control the ordered growth and differentiation of uterine cells. This remodelling process is critical for implantation of the developing embryo, the formation of the placenta, and maintenance of pregnancy. Failure of uterine tissues to respond appropriately to ovarian hormone signalling results in defective placentation, associated with a spectrum of pregnancy disorders such as recurrent miscarriages and preeclampsia. These obstetrical disorders are a major cause of maternal and perinatal morbidity and mortality. Progesterone exerts its action on target cells, at least in part, through binding to the progesterone receptor (PR), a member of the steroid/ thyroid hormone receptor superfamily of ligand-activated transcription factors. The mechanism by which progesterone controls the differentiation of human endometrial stromal cells, a process termed decidualization, in the secretory phase of the menstrual cycle is not well understood. Emerging evidence indicates that locally expressed factors and activation of the cAMP second messenger pathway integrate hormonal inputs and confer cellular specificity to progesterone action through the induction of diverse transcription factors capable of modulating PR function.

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“…We and other investigators have developed in vitro models of human endometrial stromal cell decidualization, in which the induction of deciduatype prolactin is a marker of this process (Irwin et al 1989, Tabanelli et al 1992, Frank et al 1994, Brosens et al 1996, Brar et al 1999. In this study, we have used primary cultures of endometrial stromal cells and decidual fibroblast cells in which decidualization was induced with the progesterone analog medroxyprogesterone acetate (MPA) and estradiol in combination with dibutyryl cAMP (decidual fibroblasts only).…”

Section: Introductionmentioning

confidence: 99%

An Ets motif in the proximal decidual prolactin promoter is essential for basal gene expression

Brar¹,

Kessler²,

Handwerger³

2002

Journal of Molecular Endocrinology

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Transcriptional regulation of the prolactin gene in the decidua differs from that in the pituitary. Several lines of evidence strongly suggest this difference is due to regulation of the prolactin gene in the decidua and other extra-pituitary tissues by tissue-specific transcription factors, which activate distinct promoters to induce prolactin gene expression in extra-pituitary sites compared with the pituitary. The human decidua is a major site of extra-pituitary expression of the prolactin gene. Here we present evidence that the transcription factor Ets-1 is critical for basal expression of the decidua-type (or decidual) prolactin promoter. Overexpression of Ets-1 significantly induces decidual prolactin promoter activity in BeWo and JAR cells that express little or no endogenous Ets-1. Conversely, a dominant/negative mutant of Ets represses basal promoter activity. Although the proximal 1·5 kb of the decidual prolactin promoter contains six Ets motifs, only the Ets motif at nt -77/-71 is essential for basal gene expression. Mutation of the Ets motif at nt -77/-71 results in an approximately 90% decrease in promoter activity, while mutation of the other Ets motifs results in only small changes. Electrophoretic mobility shift assays demonstrate that Ets proteins in decidualized endometrial stromal cells bind this Ets motif in the decidual prolactin promoter. Ets protein expression increases up to 20-fold upon induction of decidualization in endometrial stromal cells under conditions in which expression of the prolactin gene is also induced. These studies provide strong evidence for a critical role of the Ets transcription factor in basal expression of the decidual prolactin promoter.

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In vitro decidualization of human endometrial stromal cells

Cited by 134 publications

References 22 publications

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour

Cyclic AMP and progesterone receptor cross-talk in human endometrium: a decidualizing affair

An Ets motif in the proximal decidual prolactin promoter is essential for basal gene expression

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