In vitro degradation and controlled release behavior of ,-PLGA50 and PCL-b-,-PLGA50 copolymer microspheres

Dong, Chang‐Ming; Guo, Yingzhi; Qiu, Kun-Yuan; Gu, Zhongwei; Feng, Xia

doi:10.1016/j.jconrel.2005.05.024

Cited by 64 publications

(55 citation statements)

References 28 publications

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“…Poly(e-caprolactone) (PCL) is the biodegradable and biocompatible polyester, [1][2][3] which has many medical applications such as drug delivery 1,[4][5][6][7] and the development of tissue-engineering scaffolds. [8][9][10][11][12] However, the cells cannot easily adhere to the scaffolds because PCL does not have any cell recognition sites.…”

Section: Introductionmentioning

confidence: 99%

The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering

Chang

Hung

Chu

et al. 2009

J Biomedical Materials Res

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This study investigates a poly(epsilon-caprolactone)-graft-type II collagen-graft-chondroitin sulfate (PCL-g-COL-g-CS) biomaterial as a scaffold for cartilage tissue engineering. Biodegradable polyester, PCL, was utilized to fabricate three-dimensional (3D) porous scaffolds by particulate leaching. The PCL scaffold was then surface modified by chemical bonding of 1,6-hexanediamine and the grafting of a bioactive polymer layer of COL and CS with the help of 1-ethyl-3-(3-dimethyl- aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) on the modified PCL surface to produce PCL-g-COL and PCL-g-COL-g-CS, respectively. The characteristics of these modified and grafted matrices were examined by ESCA, aminolysis, collagen and CS assay, porosity and water-binding capacity. Grafted COL and CS markedly increased water-binding capacity, and promoted the spreading and growth of chondrocytes. During a 4-week culture period, PCL-g-COL and PCL-g-COL-g-CS matrices both provided more cell proliferation, as determined by measuring the DNA assay. Additionally, a larger amount of secreted collagen and glycosaminoglycans (GAGs) appeared in the PCL-g-COL-g-CS matrices than in the control (PCL) as indicated by the histochemical sections via Hematoxylin and eosin (H&E) stain, Masson trichrome stain and Safranin-O stain. The chondrocytes were induced to function normally; the cell phenotype was maintained, and the GAGs and collagen in the PCL-g-COL-g-CS scaffold were secreted in vitro. These results serve as a basis for future studies of the fabrication process and reveal the potential biocompatibility of the biomimetic matrix for regenerating articular cartilage or other organs.

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Section: Introductionmentioning

confidence: 99%

The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering

Chang

Hung

Chu

et al. 2009

J Biomedical Materials Res

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“…Furthermore, proteins are released out in a controllable manner rather than a simple diffusion when biodegradable polymers are employed to formulate polymeric microspheres encapsulating protein drugs [5,6]. In attempts to control the release rate of bioactive proteins encapsulated within polymeric microspheres, molecular weight and hydrophilicity of the polymer was widely modified to control degradation rates of polymeric microspheres [3,7,8]. However, those approaches were simply dependent on degradation rates of polymers, therefore, could not reflect in vivo physiological changes including temperatures, glucose levels, and pH, where pharmaceutical drug should be released according to micro-environmental changes in vivo.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable microspheres containing poly(ɛ-caprolactone)–Pluronic block copolymers for temperature-responsive release of proteins

Lee

Yoo

2008

Colloids and Surfaces B: Biointerfaces

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“…These results are consistent with Dong's report. 33 During the following 8 days, the release rate decreased to about 0.4 mg/day and then it approached complete (80%) of the PHT release from the microcarriers. The release rate in this stage became much slower than that in the first stage, and we hypothesize that release at this point was a consequence of diffusion through pores generated in the dosage form, which was formed by the coalescence of some PHT crystals, since they might stick to each other when the PCL suspensions containing PHT was spray-dried.…”

Section: Pxrd Analysismentioning

confidence: 93%

Formulation of Spray-Dried Phenytoin Loaded Poly(ε-Caprolactone) Microcarrier Intended for Brain Delivery to Treat Epilepsy

Simon

et al. 2007

Journal of Pharmaceutical Sciences

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In vitro degradation and controlled release behavior of ,-PLGA50 and PCL-b-,-PLGA50 copolymer microspheres

Cited by 64 publications

References 28 publications

The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering

The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering

Biodegradable microspheres containing poly(ɛ-caprolactone)–Pluronic block copolymers for temperature-responsive release of proteins

Formulation of Spray-Dried Phenytoin Loaded Poly(ε-Caprolactone) Microcarrier Intended for Brain Delivery to Treat Epilepsy

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