In vitro degradation of silk fibroin

Horan, Rebecca L.; Antle, Kathryn A.; Collette, Adam L.; Wang, Yongzhong; Huang, Jia; Moreau, Jodie E.; Volloch, Vladimir; Kaplan, David L.; Altman, Gregory H.

doi:10.1016/j.biomaterials.2004.09.020

Cited by 679 publications

(539 citation statements)

References 17 publications

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“…While there are a number of biomaterials available for drug delivery devices, purified silk fibroin protein is a unique option that is particularly well suited to the needs listed above. Silk fibroin is biocompatible [20] and biodegrades slowly [21]. Degradation lifetimes of silk can be regulated by the extent of physical crosslinking for beta sheet formation, allowing control of degradation timeframes from weeks to years [21], an important property for sustained release applications.…”

Section: Introductionmentioning

confidence: 99%

Silk polymer-based adenosine release: Therapeutic potential for epilepsy

et al. 2008

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Adenosine augmentation therapies (AAT) make rational use of the brain's own adenosine-based seizure control system and hold promise for the therapy of refractory epilepsy. In an effort to develop an AAT compatible with future clinical application, we developed a novel silk protein-based release system for adenosine. Adenosine releasing brain implants with target release doses of 0, 40, 200, and 1,000 ng adenosine/day were prepared by embedding adenosine-containing microspheres into nanofilm-coated silk fibroin scaffolds. In vitro, the respective polymers released 0, 33.4, 170.5, and 819.0 ng adenosine/day over 14 days. The therapeutic potential of the implants was validated in a dose-response study in the rat model of kindling epileptogenesis. Four days prior to the onset of kindling, adenosine-releasing polymers were implanted into the infrahippocampal cleft and progressive acquisition of kindled seizures was monitored over a total of 48 stimulations. We document a dose-dependent retardation of seizure acquisition. In recipients of polymers releasing 819 ng adenosine/day, kindling epileptogenesis was delayed by one week corresponding to 18 kindling stimulations. Histological analysis of brain samples confirmed correct location of implants and electrodes. We conclude that silk-based delivery of around 1,000 ng adenosine/day is a safe and efficient strategy to suppress seizures.

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Section: Introductionmentioning

confidence: 99%

Silk polymer-based adenosine release: Therapeutic potential for epilepsy

et al. 2008

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“…[103,104] Silk is degradable and lightweight with excellent thermal and mechanical properties. [105,106] SF is obtained after the extraction of sericin proteins from silk. SF is non-immunogenic, biocompatible, and capable of supporting cell attachment, spreading, growth, and differentiation.…”

Section: Sfmentioning

confidence: 99%

Graphene Oxide/Polymer‐Based Biomaterials

2017

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Since its discovery in 2004, derivatives of graphene have been developed and heavily investigated in the field of tissue engineering. Among the most extensively studied forms of graphene, graphene oxide (GO), and GO/ polymer-based nanocomposites have attracted great attention in various forms such as films, 3D porous scaffolds, electrospun mats, hydrogels, and nacre-like structures. In this review, the most actively investigated GO/ polymer nanocomposites are presented and discussed, these nanocomposites are based on chitosan, cellulose, starch, alginate, gellan gum, poly(vinyl alcohol) (PVA), poly(acrylamide), poly(e-caprolactone) (PCL), poly(lactic acid) (PLLA), poly(lactide-co-glycolide) (PLGA), gelatin, collagen, and silk fibroin (SF). The biological and mechanical performance of such nanocomposites are comprehensively scrutinized and ongoing research questions are addressed. The analysis of the literature reveals overall the great potential of GO/ polymer nanocomposites in tissue engineering strategies and indicates also a series of challenges requiring further research efforts.

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“…Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al, 2005;Meinel et al, 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.…”

Section: Introductionmentioning

confidence: 99%

“…Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al, 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al, 2005;Meinel et al, 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.…”

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confidence: 99%

Cytocompatibility of silkworm cocoon layer extracts

Jo¹,

Kim²,

Lee³

et al. 2016

International Journal of Industrial Entomology

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Recently silk polymer produced by Bombyx mori silkworm has been considered as biological macromolecules. Silk polymer was extracted in PBS solution at 37 o C for 72 h or 72 o C for 24 h. The effect of EtOH treatment on the cocoon extraction was also examined. The extraction yield of cocoon was less than 1 wt% regardless of extraction conditions. UV spectroscopy showed that the experimental extracts have absorption bands at 280 nm. There is no cytotoxicity effect on the mouse fibroblast L929 cell. The phenotype of L929 cell was not changed under the experimental conditions. The proliferation behavior of L929 cell was not affected by the addition of cocoon extract. Therefore, cocoon extract might be cytocompatible and can be used as promising biomaterials. IntroductionA silkworm cocoon of Bombyx mori provides silk fiber for textile industries for more than 4,000 years. Recently, silk polymer has been revealed several advantages for biological materials and then applied various functional materials such as cosmetics, suture and artificial eardrum (Kundu et al., 2013;Ju et al., 2014;Vepari and Kaplan, 2007; Kim et al., 2010), and so on.In the view point of material, silk is a natural protein polymer composed of two proteins: one is a central protein called silk fibroin and another is gum-like protein called silk sericin. Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al., 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al., 2005;Meinel et al., 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.Silk sericin is a protein polymer, which acts as an adhesive binder to maintain the structure of the fibers. Sericin is an albuminoid protein composed of serine (30%) and other amino acids (Teramoto and Miyazawa, 2005;Garel et al., 1997). Sericin was removed easily from Bombyx mori cocoon using a thermo or chemical process called degumming (Mo et al., 2009) Preparation of cocoon extractsEach cocoon was washed with tap water and then dried absolutely with room temperature in the dark. The cocoon was sliced into 5 mm ⅹ 5 mm pieces. Each cocoon slices were divided into two groups; one group was treated with absolute ethyl alcohol (EtOH) for 24 h at R.T. and another group was not treated EtOH. After treatment, cocoon was dried with R.T. for overnight. The extraction condition were followed; weigh 1 g of cocoon and add into 10 mL of 1X PBS buffer at 37 o C for 72 h and/or 72 o C for 24 h. Save the cocoon extracts and then filtered it with 0.22 um syringe filter (Sartorius Ltd., Epsom, UK) and protein concentration of all extracts was determined by bicinchronic acid (BCA) assay. Forty ug of each extracts used for cell cytotoxicity and proliferation assay. UV vis sp...

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In vitro degradation of silk fibroin

Cited by 679 publications

References 17 publications

Silk polymer-based adenosine release: Therapeutic potential for epilepsy

Silk polymer-based adenosine release: Therapeutic potential for epilepsy

Graphene Oxide/Polymer‐Based Biomaterials

Cytocompatibility of silkworm cocoon layer extracts

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