In vitro determination of hemoglobin A1c for diabetes diagnosis and management: technology update

English, Emma; John, W. Garry; Milosevich, Elise

doi:10.2147/plmi.s48403

Cited by 6 publications

(1 citation statement)

References 48 publications

(31 reference statements)

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“…Glycation of the N-terminal residue changes its structure and decreases the positive charge of HbA. As a result, a slight difference in isoelectric point between HbA1c and HbA0 allows them to be separated by charge, while the presence of the glucose adduct on HbA1c allows for separation by structural difference (2). Thus, methods of HbA1c analysis can be divided into two categories: those based on the charge differences (such as in cation-exchange chromatography, electrophoresis, and isoelectric focusing) and those based on the structural differences (such as in boronate afﬁnity chromatography and immunoassay) (2).…”

Section: Discussionmentioning

confidence: 99%

Spurious HbA1c results in patients with diabetes treated with dapsone

Aljenaee

Hakami

Davenport

et al. 2019

Endocrinology, Diabetes &Amp; Metabolism Case Reports

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Summary Measurement of glycated haemoglobin (HbA1c) has been utilised in assessing long-term control of blood glucose in patients with diabetes, as well as diagnosing diabetes and identifying patients at increased risk of developing diabetes in the future. HbA1c reflects the level of blood glucose to which the erythrocyte has been exposed during its lifespan, and there are a number of clinical situations affecting the erythrocyte life span in which HbA1c values may be spuriously high or low and therefore not reflective of the true level of glucose control. In the present case series, we describe the particulars of three patients with diabetes who had spuriously low HbA1c levels as a result of dapsone usage. Furthermore, we discuss the limitations of HbA1c testing and the mechanisms by which it may be affected by dapsone in particular. Learning points: Various conditions and medications can result in falsely low HbA1c. Dapsone can lead to falsely low HbA1c by inducing haemolysis and by forming methaemoglobin. Capillary glucose measurement, urine glucose measurements and fructosamine levels should be used as alternatives to HbA1c for monitoring glycaemic control if it was falsely low or high.

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Section: Discussionmentioning

confidence: 99%

Spurious HbA1c results in patients with diabetes treated with dapsone

Aljenaee

Hakami

Davenport

et al. 2019

Endocrinology, Diabetes &Amp; Metabolism Case Reports

View full text Add to dashboard Cite

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Anti‐Diabetic and Insulinotropic Effects of p‐Anisic Acid in High‐Fat Diet and Streptozotocin Induced Type‐2 Diabetic Rats

Vora,

Tirgar,

Raval

2024

Chemistry & Biodiversity

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Purpose: To evaluate the antidiabetic effect of p‐anisic acid (p‐AA) against type 2 diabetes mellitus (T2DM). Methods: Thirty‐six male rats were utilized for the study. The animals were provided with the high‐fat diet (HFD) and T2DM was induced through 35 mg/kg streptozotocin (STZ). Subsequently, the animals were allocated to 6 groups and subjected to the treatments for 4 weeks followed by a 2‐week observation. Three treatment groups were administered with p‐AA (25 mg/kg, 50 mg/kg or 100 mg/kg). Glibenclamide (3 mg/kg) was provided to the standard control group. The normal control and disease control groups were supplied with 0.1 % carboxymethyl cellulose (CMC). Subsequently, the effects of treatment on body weight (BW), blood glucose level (BGL), glycosylated haemoglobin (HbA1c), insulin, and lipid parameters were measured and the pancreas was isolated for histopathology. Results: A statistically significant (P‐value<0.001) increase in BW was observed in the p‐AA (50 mg/kg and 100 mg/kg) groups. The BGL decreased significantly (P‐value<0.001) in p‐AA groups. p‐AA significantly (P‐value<0.001) regulated the levels of HbA1c and serum insulin and lipid parameters. A notable improvement in the morphology of the pancreas was observed. Conclusion: Treatment with p‐AA exhibited significant antidiabetic effects against STZ‐HFD‐induced T2DM.

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