In vitro differentiation of human dental follicle cells with dexamethasone and insulin

Morsczeck, Christian; Moehl, C.; Götz, Werner; Heredia, Ascensión; Schäffer, Tilman E.; Eckstein, Niels; Sippel, Claudia; Hoffmann, Karen

doi:10.1016/j.cellbi.2005.03.020

Cited by 105 publications

(84 citation statements)

References 25 publications

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“…BMPs, and more specifically BMP-2, have potent effects in inducing osteoblast differentiation of different cell sources [14,19,20,46,53]. Several studies have demonstrated dexamethasone (DEX) as a non-GF can induce terminally differentiated osteoblasts [18,36,42].…”

Section: Introductionmentioning

confidence: 99%

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Balmayor

Feichtinger

Azevedo

et al. 2009

Clin Orthop Relat Res

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BMP-2 is currently administered clinically using collagen matrices often requiring large amounts of BMP-2 due to burst release over a short period of time. We developed and tested a novel injectable drug delivery system consisting of starch-poly-e-caprolactone microparticles for inducing osteogenesis and requiring smaller amounts of BMP-2. We evaluated BMP-2 encapsulation efficiency and the in vitro release profile by enzyme-linked immunosorbent assay. BMP-2 was rapidly released during the first 12 hours, followed by sustained release for up to 10 days. We then evaluated the osteogenic potential of dexamethasone (standard osteogenic induction agent) and BMP-2 after incorporation and during release using an osteo/myoblast cell line (C2C12). Alkaline phosphatase activity was increased by released BMP-2. Mineralization occurred after stimulation with BMP-2-loaded microparticles. A luciferase assay for osteocalcin promoter activity showed high levels of activity upon treatment with BMP-2-loaded microparticles. In contrast, no osteogenesis occurred in C2C12 cells using dexamethasone-loaded microparticles. However, human adipose stem cells exposed to the microparticles produced high amounts of alkaline phosphatase. The data suggest starch-poly-e-caprolactone microparticles are suitable carriers for the incorporation and controlled release of glucocorticoids and growth factors. Specifically, they reduce the amount of BMP-2 needed and allow more sustained osteogenic effects.

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Section: Introductionmentioning

confidence: 99%

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Balmayor

Feichtinger

Azevedo

et al. 2009

Clin Orthop Relat Res

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“…These results are comparable to our previous investigations. Here, OCN was also imbedded in the extracellular matrix of DFCs after the osteogenic differentiation (Morsczeck et al 2005b). We suppose that DFCs secrete constitutively OCN into the cell culture medium.…”

Section: Discussionmentioning

confidence: 99%

The extracellular concentration of osteocalcin decreased in dental follicle cell cultures during biomineralization

2016

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The secretion of osteocalcin (OCN) is an excellent differentiation marker for the osteogenic differentiation. This study investigated the secretion of OCN during the osteogenic differentiation of DFCs. During the differentiation of DFCs the extracellular concentrations of OCN were higher in standard cell culture medium than in osteogenic differentiation medium. However, after 4 weeks in the osteogenic differentiation medium the extracellular OCN concentration decreased strongly, whereas the concentration remains high in the control medium. At this point in time DFCs formed connective tissue like structures with mineralized clusters and OCN. Real-time RT-PCR analyses and western-blot analyses proved that OCN was expressed in both cell culture media. However, the expression of the mRNA was inhibited in the osteogenic differentiation medium. These results suggest that DFCs secrete constitutively OCN into the cell culture medium and that the osteogenic differentiation medium suppresses the gene expression of OCN. Moreover, OCN imbeds into the extracellular matrix after the formation of connective tissue like structures, and the soluble OCN in the cell culture medium disappears. Hence, extracellular OCN in the cell culture medium is not a marker for the osteogenic differentiation of DFCs.

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“…The gene expression of osteoprotegerin (OPG) reduced in the dental follicle of the first mandibular molar of the rat at day 3 postnatally, then expressed consistently up to day 10 ( Wise et al, 2000). In fact, there have been many studies which have concentrated on the important molecules promoting the differentiation of dental follicle cells in vitro (Zhao et al, 2002;Morsczeck et al, 2005;Chen et al, 2007;Kémoun et al, 2007). With the concerns above, detection methods both in vivo and vitro were included in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that vascular endothelial growth factor (VEGF), dexamethasone/insulin, bone morphogenetic protein 2 (BMP2) and bone morphogenetic protein 7 (BMP7) can promote cementoblast/osteoblast differentiation of DFCs (Zhao et al, 2002;Morsczeck et al, 2005;Chen et al, 2007;Kémoun et al, 2007). In tooth eruption, cytokines such as colony-stimulating factor 1 (CSF-1) and its receptor, interleukin-1 (IL-1) and its receptor, endothelial growth factor (EGF), monocyte chemoattractant protein 1 (MCP-1) and nuclear factor-κB (NF-κB) were noted for their involvement in the dental follicle, in which they combine to form a complicated tooth eruption network system (Lin et al, 1996;Wise et al, 1997;Xu et al, 1998;Que et al, 1998;Grier et al, 1998;Que et al, 1999;Gu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%