In vitro digestibility of dietary protein sources for white shrimp (Penaeus vannamei)

In vitro protein digestibility of animal, vegetal and microbial feed ingredients for Macrobrachium tenellum

“…They found the highest values on yeast, beef blood meal, and pork meal; in contrast, the lowest digestibility values were found on soybean paste, fish meal, and fish hydrolyzate. In general, relative digestibility and protein hydrolysis values were higher (88.3-189.0% and 27.8-59.5%, respectively), than those reported in other studies (Ezquerra et al, 1998;Lemos et al, 2004;Nieto et al, 2005).…”

“…This depends on many other factors such as the physical properties (i.e., particle size, solubility, etc. ), and chemical properties (i.e., amino acid content); but also biological characteristics of the animal; the architecture of the digestive tract, and physiology will affect protein digestibility, and also environmental conditions are important (pH, temperature, salinity, ions); all these aspect should be considered for the determination of in vitro protein digestibility of ingredients or feed (Cruz-Suárez, 1996;Ezquerra et al, 1998;Carrillo-Farnés et al, 2007). However, most of the new protein ingredients have not been assessed from the digestibility point of view (Álvarez-González, 2003).…”

“…The pH-stat in vitro method has been widely used in the evaluation of the nutritional quality of raw materials and processed feeds, because it is simple, fast, and with high reproducibility (Moyano et al, 2014). The use of pHstat system has proved to be suitable for checking the quality of the protein sources, for marine shrimp feed formulation (Ezquerra et al, 1998;Lemos et al, 2004Lemos et al, , 2009.…”

Species specific in vitro protein digestion (pH-stat) for fish: method development and application for juvenile rainbow trout (Oncorhynchus mykiss), cobia (Rachycentron canadum), and Nile tilapia (Oreochromis niloticus)

“…This motivated the development of in vitro methods based on the digestion of small amounts of feedstuff samples. Reported in vitro protein digestion methods include the use of digestive enzymes that may be available from commercial sources (Lazo et al, 1998;Shipton and Britz, 2002;Tonheim et al, 2007) or recovered from the target species Ezquerra et al, 1998;Tibbetts et al, 2011a). Nevertheless, different results in the in vitro digestion have been found depending on the enzyme origin, suggesting a species-specific feature of in vitro protein digestion (Ezquerra et al, 1998;Lemos and Nunes, 2008;Moyano and Savoie, 2001).…”

In vivo and in vitro protein digestibility of formulated feeds for Artemesia longinaris (Crustacea, Penaeidae)

“…Studies focused to ascertain the nutrients digestibility coefficients usually consist of feeding a feed containing fixed concentrations of a nutrient and an indigestible marker to measure in the feces (Fenucci et al, 1980;Divakaran et al, 2002). Species specific in vitro digestibility methods have been developed using digestive enzymes from the species in question (Lan and Pan, 1993;Ezquerra et al, 1998;Ramos Díaz et al, 2001;Lemos et al, 2004). These methods are usually faster and less expensive than in vivo methods.…”