In vitro digestion of polysaccharides: InfoGest protocol and use of small intestinal extract from rat

Digestion of pectic polysaccharide from Brassica rapa L. in vitro and its effect on the intestinal microbiota in cyclophosphamide‐treated mice

“…The significant decrease in M w of BRP might be due to its sensitivity to acidic pH, resulting in the breakage of some glycosidic bonds (Hu et al ., 2013). It was also reported that if the decrease in M w is caused by the breakage of glycosidic bonds, then the number of reducing ends increases, which coincides with the trend of C R in this study, suggesting that the decrease in molecular weight might be due to the breakage of glycosidic bonds (Ding et al ., 2019a). Furthermore, the peak delayed with digestion time was also observed in stimulated intestinal digestion, which also meant a reduction of the M w in stimulated intestinal digestion.…”

“…The small intestinal digestion was conducted according to the published reports (Ding et al ., 2019a; Gallego‐Lobillo et al ., 2021). The simulated small intestinal digestion (SIES) was made up of 540 mg NaCl, 65 mg KCl, 33 mg CaCl 2 , 100 mg pancreatin and 100 mL distilled water, and the pH was adjusted to 7.0 by NaHCO 3 (0.1 M).…”

“…The sample was subjected to in vitro digestion according to the reported literatures (Ding et al ., 2019a; Gallego‐Lobillo et al ., 2021); 40.0 mg BRP was dissolved in 20.0 mL gastric juice and then incubated in a water bath at 37 °C for 6 h. The pH of mixed fluid was maintained to 2.0 by HCl (0.1 M) and the samples were collected at 0, 3 and 6 h of digestion after boiling in water to inactivate enzymes. The gastric digestion of the sample (2.0 mg mL −1 ) was incubated in water bath at 37 °C for 6 h. Then, the aforementioned gastric digested solution at 6 h was mixed with the SIES at a ratio of 1:1, and the resulting mixture was adjusted the pH to 7.0 by 0.1 M NaHCO 3 solution.…”

Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro

“…As reported, some macromolecular polysaccharides from natural sources can be digested by saliva or gastrointestinal fluid, and some cannot [ 10 ]. However, studies on the digestibility of polysaccharides even from same species offer conflicting conclusions.…”

Glycated α-lactalbumin based micelles for quercetin delivery: Physicochemical stability and fate of simulated digestion

“…Therefore, ALA was suitable for investigating the improvement of glycation on encapsulation capacity and digestion resistance. Dextran is a glucose polymer mainly formed by α-1,6-glycosidic linkages with good water solubility, commercially selectable molecular weight and high resistance to mammalian digestion ( Gallego-Lobillo, Ferreira-Lazarte, Hernández-Hernández, & Villamiel, 2021 ). Dextran is widely used for protein glycosylation to obtain conjugates with different grafting degrees (GDs) and good EE for lipophilic bioactive compounds ( Fan, Yi, Zhang, Wen, & Zhao, 2017 ).…”