In Vitro Drug Release and Biocatalysis from pH-Responsive Gold Nanoparticles Synthesized Using Doxycycline

Abstract

pH-sensitive doxycycline gold nanoparticles (doxy-AuNPs) are reported here to act as effective drug nanocarriers and as biocatalysts. The AuNPs were synthesized with doxy as the reducing and capping agent. Various parameters were optimized to find the best conditions for the synthesis of doxy-AuNPs, and these were characterized with UV−vis, X-ray diffraction (XRD), FTIR, and transmission electron microscopy (TEM). Doxy-AuNPs were then loaded with the anticancer drug doxorubicin (DOX), where 70% of the initially available drug was loaded within 24 h. Furthermore, pH-dependent drug release was measured at 60% with in vitro measurements in phosphate-buffered saline (PBS). In addition, the doxy-AuNPs were applied as a biocatalyst. Oxidation of dopamine was taken as a model reaction to determine the catalytic activity of doxy-AuNPs. Almost complete oxidation of dopamine occurred in 5 min, which indicates the fast response of synthesized doxy-AuNPs as a biocatalyst. Hence, doxy-AuNPs are a versatile platform for drug loading and biocatalyst.

Minocycline-Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan-Induced Diabetic Mice

“…Nanobiotechnology is among the demanding areas of research that make use of biological substances at nanoscale and find applications for them in different fields, such as biosensors [10], diagnostics [11], bio-imaging [12], catalysis [13], drug delivery systems [14], and nanomedicine [15]. In comparison to conventional drug formulations, nanomedicine has received more attention in the last few years due to its benefits, such as more precise diagnosis, a higher percentage of recovery, and more effective therapies [16].…”

Hollow Gold Nanoparticles Loaded with L-Buthionine-Sulfoximine as a Novel Nanomedicine for In Vitro Cancer Cell Therapy

“…Otherwise, it is reported that cancer-specific conditions such as acidic pH and higher levels of glutathione, heat, or light can be used to trigger the release of the drug from the nanocarrier [36]. These parameters act as stimuli for the smart release of the drug [36,37]. However, power evidences are needed to further clarify whether "BSO is released from BSO@HAuNS."…”

Minocycline Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan induced Diabetic Mice

“…All animal trial techniques were directed as per local and worldwide controls. The nearby direction is the Wet op de dierproeven (Article 9) of Dutch Law (International) as detailed in our previous studies Hussain et al 2020;Ara et al 2020;Khan et al 2019;Ali et al 2019;Mumtaz et al 2019;Mughal et al 2019;Dar et al 2019) and The Institutional Bioethics Committee at Government College University Lahore, Pakistan (No. GCU/IIB/21 dated: 08-01-2019).…”

“…Nanobiotechnology is among the demanding areas of research that make use of the biological substances at the nanoscale and find their applications in different fields such as biosensor (Kazmi et al, 2020), diagnostics (Hu et al, 2013), bio-imaging (Zhou et al, 2018), catalysis (Raza et al, 2017), drug delivery system (Kazmi et al, 2019) and nanomedicine (Wicki et al, 2015). In comparison to the conventional drug formulations, nanomedicine has retrieved more attention for the last few years due to its benefits such as more precise diagnosis, a higher percentage of recovery, and more effective therapies (Kouame et al, 2019).…”