In vitro effect of dehydroepiandrosterone sulfate on steroid receptors, aromatase, cyclooxygenase-2 expression, and steroid hormone production in preovulatory human granulosa cells

ELBeltagy, Khalid; Honda, Kenichi; Ozaki, Koji; Misugi, Takuya; Tokuyama, Osamu; Kimura, Michio; Kira, Yukimi; Ishiko, Osamu

doi:10.1016/j.fertnstert.2007.01.008

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…According to this finding, it has been reported that an excess of androgens leads to more prolonged sexual cycles in PCOS patients by rescuing the CL from luteolysis (Manikkam et al 2006). The ability of DHEA to stimulate E 2 secretion has also been previously reported in human granulose cells (Bonser et al 2000), and the mechanism proposed is the enhanced expression of P450 aromatase by DHEA (Elbeltagy et al 2007). Moreover, Genazzani et al (2006) have recently reported that administration of DHEA in postmenopausal women is capable of increasing circulating levels of androgens and progestins.…”

Section: Discussionmentioning

confidence: 74%

Effect of DHEA and metformin on corpus luteum in mice

Sander¹,

Facorro²,

Piehl³

et al. 2009

Reproduction

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We evaluated the effect of hyperandrogenism in ovaries with functional and regressing corpora lutea (CL) and the action of metformin in preventing these possible alterations using a mouse model. To obtain a CL functional for 9G1 days, immature female mice of the BALB/c strain were injected i.p. with 10 IU/mouse of pregnant mare's serum gonadotropin (PMSG). DHEA (60 mg/kg body weight s.c., 24 and 48 h prior to kill) decreased both serum progesterone (P) and estradiol (E 2 ) levels and increased the activity of superoxide dismutase (SOD) from ovaries with functional CL (on day 5 after PMSG). It increased P and E 2 and the activities of SOD and catalase (CAT) and decreased lipoperoxidation of ovaries with regressing CL (on day 9 after PMSG). Treatment with DHEA did not affect the production of prostaglandin F 2a (PGF 2a ) or PGE by ovaries with functional CL, whereas DHEA decreased PGF 2a and increased PGE production by ovaries with regressing CL. Metformin (50 mg/kg body weight, orally) given together with DHEA restored E 2 levels from mice with ovaries with functional CL and serum P, PGF 2a and PGE levels, and oxidative balance in mice with ovaries with regressing CL. Metformin alone was able to modulate serum P and E 2 levels, lipoperoxidation, SOD and CAT, and the 5,5-dimethyl-1-pyrroline N-oxide/ % OH signal. These findings suggest that hyperandrogenism is able to induce or to rescue CL from luteolysis and metformin treatment is able to prevent these effects.

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Section: Discussionmentioning

confidence: 74%

Effect of DHEA and metformin on corpus luteum in mice

Sander¹,

Facorro²,

Piehl³

et al. 2009

Reproduction

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“…Theses levels were particularly high with respect to the concentrations usually found (Van Eenoo et al, 2001; Hemmersbach et al, 2006). For this woman, further analysis detected a high serum level of DHEAs, which may have induced an increase in ovarian aromatase activity (ElBeltagy et al, 2007).…”

Section: Discussionmentioning

confidence: 96%

Nandrolone excretion in sedentary vs physically trained young women

Enéa¹,

Bayle²,

FLAMENT³

et al. 2010

Scandinavian Med Sci Sports

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We investigated the effects of the menstrual cycle, oral contraception and physical training on exhaustive exercise-induced changes in the excretion of nandrolone metabolites [19-norandrosterone (19-NA), and 19-noretiocholanolone (19-NE)] in young women. Twenty-eight women were allocated to an untrained group (n=16) or a trained group (n=12), depending on their physical training background. The untrained group was composed of nine oral contraceptive users (OC+) and seven eumenorrheic women (OC-), while the trained group was entirely composed of OC+ subjects. Three laboratory sessions were conducted in a randomized order: a prolonged exercise test, a short-term exercise test and a control session. Urine specimens were collected before and 30, 60 and 90 min after the exercise test and at the same times of the day during the control session. Urinary concentrations of nandrolone metabolites were determined by gas chromatography coupled to mass spectrometry. Urinary concentrations of 19-NA and 19-NE ranged from undetectable levels to 1.14 and 0.47 ng/mL, respectively. Nandrolone excretion was not affected by the menstrual cycle phase (early follicular vs mid-luteal), prior physical training, oral contraception or acute physical exercise. Therefore, a urinary concentration of 2 ng/mL of 19-NA appears to be fair as the upper acceptable limit in doping control tests for female athletes.

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“…However, this possible association requires further evaluation. Elbetagy et al [23] treatment of granulosa cells with low dose DHEAS increased the expression of androgen receptor, aromatase, 3-beta-hydroxysteroid dehydrogenase, and cyclooxygenase-2, reduced the expression of estrogen receptor beta, and increased estrone and estradiol levels. To sum up, DHEA not only has a beneficial effect on the percentages of primordial follicles but also affects the steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

Effect of dehydroepiandrosterone on ovarian morphology and follicular apoptosis following 4-vinylcyclohexene diepoxide induced premature follicle loss in rats

Özer¹

2020

Ann Clin Anal Med

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Dehydroepiandrosterone and premature ovarian loss in a rat model Effect of dehydroepiandrosterone on ovarian morphology and follicular apoptosis following 4-vinylcyclohexene diepoxide induced premature follicle loss in rats AbstractAim: Chemical-induced depletion of ovarian follicle pool can lead to premature ovarian failure in rats. In the rat model of 4-vinylcyclohexene diepoxide (VCD)induced ovarian follicular loss, we tested the hypothesis that Dehydroepiandrosterone (DHEA) could be reversed by short and long-term treatment of follicular loss. Material and Method: All rats were divided into groups for study and treated with dimethylsulfoxide (DMSO) (control group), VCD alone, DHEA and VCD + DHEA for only 15 days. At the end of 15 days, some of the rats were killed as a group of 15 days. Others continued their treatment with DHEA to form groups of 35 and 55 days, respectively. For each animal, follicle numbers were counted and photographed. Results: VCD treatment produced a sustainable state of ovotoxicity throughout the experiment, diminishing the numbers of both primordial and primary follicles (p<0.05). Only VCD-treated rats showed apoptotic cells at very high rates with TUNEL and caspase-3 immunohistochemical staining (p <0.05). On 15th day, concurrent use of DHEA increases the percentages of both normal primordial and primary follicles. More cystic follicles were observed in both VCD-and DHEA-treated rats, compared to controls (p<0.05). Discussion: In this ovotoxicity model with VCD in rats, administration of DHEA provides protection in the number of primordial follicles in the short and long term.

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In vitro effect of dehydroepiandrosterone sulfate on steroid receptors, aromatase, cyclooxygenase-2 expression, and steroid hormone production in preovulatory human granulosa cells

Abstract: DHEAS may be an essential trigger of ovulation.

Cited by 11 publications

References 31 publications

Effect of DHEA and metformin on corpus luteum in mice

Effect of DHEA and metformin on corpus luteum in mice

Nandrolone excretion in sedentary vs physically trained young women

Effect of dehydroepiandrosterone on ovarian morphology and follicular apoptosis following 4-vinylcyclohexene diepoxide induced premature follicle loss in rats

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