In Vitro Effect of Interferon on Development and Function of Bone‐Marrow‐Derived Cytotoxic Diffuse Colonies

Mh, Claësson

doi:10.1111/j.1365-3083.1984.tb00903.x

Cited by 3 publications

(1 citation statement)

References 9 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lohmann-Matthes et al described how cells differentiating to promonocytes in response to M-CSF have functional characteristics comparable to NK cells capable of mediating antibody-dependent cellular cytotoxicity [17,18,20,25]. Cl~sson et al have also described that bone-marrow-derived diffuse colonies induced by M-CSF, the majority of which are morphologically defined as myelomonocytic cells, have NK cell activity [4,5] and that the activity is enhanced by interferon [3]. Other investigators have shown that NK cells [13,15,16,29] or natural cytotoxic cells [7,12] can be generated from bone marrow by interferon or interleukin 2 in vitro and in vivo [23,24].…”

Section: Discussionmentioning

confidence: 98%

Induction of tumoricidal macrophages from bone marrow cells of normal mice or mice bearing a colony-stimulating-factor-producing tumor

Hosoe

Ogura

Hayashi

et al. 1989

Cancer Immunol Immunother

View full text Add to dashboard Cite

Nonadherent cells of the bone marrow of C3H/HeN mice were incubated for 3 days with the culture supernatant of an L-929 cell line containing macrophage-colony-stimulating factor. Approximately, 70% of the cells became phagocytic, adherent to plastic dishes and positive for nonspecific esterase staining. The adherent cells exhibited a weak tumoricidal activity against syngeneic mammary carcinoma cells, and the cytotoxicity was strongly augmented by the addition of bacterial lipopolysaccharide to the cytotoxicity assay. The cytotoxicity induced by lipopolysaccharide was also shown to be mediated by Thy1.2- and asialo-GM1+ cells, and was abrogated by the addition of carrageenan. Macrophage-colony-stimulating-factor-producing (D66) and nonproducing (A23) variants were separated from the MM48 tumor line in in vitro culture following limiting dilution. There was no difference between these two variants in either the in vitro growth rate or the susceptibility to macrophage-mediated cytotoxicity. C3H/HeN mice inoculated i.p. with D66 survived longer than did those inoculated i.p. with A23. C3H/HeN mice bearing D66 or A23 as an ascitic form were given i.p. injections of Nocardia rubra cell wall skeleton (N-CWS). N-CWS significantly prolonged the survival period of mice bearing D66, whereas it exhibited no apparent antitumor effect on mice bearing A23. The increase in the cell number of D66 in the peritoneal cavity was significantly retarded, compared with that of A23. In contrast, the number of peritoneal macrophages increased more in D66-bearing mice than in A23-bearing mice. The increase in the peritoneal macrophage number was further augmented by an i.p. injection of N-CWS. Peritoneal macrophages of D66-bearing mice exhibited apparent tumoricidal activity against MM48 tumor cells in the presence of lipopolysaccharide, and the cytotoxicity was significantly augmented by i.p. injection of N-CWS. On the other hand, the responsiveness of peritoneal macrophages to lipopolysaccharide was found to be poor in A23-bearing mice and the tumoricidal activity was only weakly augmented by N-CWS. These results strongly suggest that M-CSF plays an important role not only in the maturation of macrophage progenitors but also in the induction and the accumulation of activated macrophages.

show abstract

Section: Discussionmentioning

confidence: 98%

Induction of tumoricidal macrophages from bone marrow cells of normal mice or mice bearing a colony-stimulating-factor-producing tumor

Hosoe

Ogura

Hayashi

et al. 1989

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

Characterization of cytotoxic cells generated from in vitro cultures of murine bone marrow cells

Klimpel

Sarzotti

Reyes

et al. 1985

Cellular Immunology

View full text Add to dashboard Cite

Alpha-Interferon: Differential Suppression of Colony Growth from Human Erythroid, Myeloid, and Megakaryocytic Hematopoietic Progenitor Cells

Mazur¹,

Richtsmeier²,

South³

1986

Journal of Interferon Research

View full text Add to dashboard Cite

The effect of varying concentrations of interferon-alpha (IFN-alpha) on the in vitro colony growth from all single-lineage human hematopoietic colony-forming progenitor cells was evaluated. IFN-alpha was tested at concentrations of 0, 2, 20, and 200 U/ml in optimally stimulated bone marrow cultures from each of 4 volunteer donors. Substantial donor-to-donor variability and distinct, lineage-specific patterns of stem cell sensitivity to IFN-alpha were observed. In the erythroid series, the more primitive progenitor or burst-forming unit (BFU-E) was substantially more resistant to growth inhibition at low IFN-alpha concentrations than the mature colony-forming unit (CFU-E). Colony growth by the megakaryocyte progenitor cell (CFU-Meg) was decreased by all concentrations of IFN-alpha which produced a biphasic, inhibitory dose response. The response of the colony-forming unit granulocyte/macrophage (CFU-GM) was heterogeneous among the donors tested. CFU-GM growth from 2 donors was insensitive to IFN-alpha at all concentrations. Conversely, CFU-GM from the other 2 donors manifested a steep dose-response curve that was similar to that of the CFU-E. These data demonstrate a heterogeneity of progenitor cell sensitivity to growth suppression by IFN-alpha which appears to be influenced by (i) hematopoietic lineage, (ii) degree of differentiation of the progenitor cell, and (iii) individual variability.

show abstract

In Vitro Effect of Interferon on Development and Function of Bone‐Marrow‐Derived Cytotoxic Diffuse Colonies

Cited by 3 publications

References 9 publications

Induction of tumoricidal macrophages from bone marrow cells of normal mice or mice bearing a colony-stimulating-factor-producing tumor

Induction of tumoricidal macrophages from bone marrow cells of normal mice or mice bearing a colony-stimulating-factor-producing tumor

Characterization of cytotoxic cells generated from in vitro cultures of murine bone marrow cells

Alpha-Interferon: Differential Suppression of Colony Growth from Human Erythroid, Myeloid, and Megakaryocytic Hematopoietic Progenitor Cells

Contact Info

Product

Resources

About