In vitro effect of metal ions on the activity of two amphibian glyceraldehyde-3-phosphate dehydrogenases: potential metal binding sites

Mounaji, Khadija; Vlassi, Metaxia; Erraiss, Nour-Eddine; Wegnez, Maurice; Serrano, Aurelio; Soukri, Abdelaziz

doi:10.1016/s1096-4959(03)00051-4

Cited by 19 publications

(17 citation statements)

References 36 publications

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“…These three sites per subunit is consistent with the observation of 10 Zn 2+ bound per GAPDH [64]. The fourth site proposed by Mounaji and coworkers [63] is species-specific and even different between Xenopus laevis and Pleurodeles waltl. Regarding the effects of Zn 2+ of oligomeric properties, two residues just mentioned (i.e.…”

Section: Factors Affecting Oligomerizationsupporting

confidence: 89%

“…Zn 2+ also exerts an inhibitory effect on GAPDH [63], but it is not known if this action alters subunit associations. The possible role of Zn 2+ in GAPDH structure and function remains virtually an unexplored area.…”

Section: Factors Affecting Oligomerizationmentioning

confidence: 99%

“…While their sequences are similar, the structural folds are very much alike. Mounaji and coworkers [63] identified four binding sites per subunit using a motif search procedure in the GAPDHs from Xenopus laevis and Pleurodeles waltl. One of the sites is the active center, which contains a cysteine and histidine residues (i.e.…”

Section: Factors Affecting Oligomerizationmentioning

confidence: 99%

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Dynamic Oligomeric Properties

Seidler

2012

GAPDH: Biological Properties and Diversity

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This chapter provides a foundation for further research into the relationship between dynamic oligomeric properties and functional diversity. The structural basis that underlies the conformational sub-states of the GAPDH oligomer is discussed. The issue of protein stability is given a thorough analysis, since it is well-established that the primary strategy for protein oligomerization is to stabilize conformation. Several factors that affect oligomerization are described, including chemical modification by synthetic reagents. The effects of native substrates and coenzymes are also discussed. The curious feature of chloride ions having a de-stabilizing effect on native GAPDH structure is described. Additionally, the role of adenine dinucleotides in tetramer-dimer equilibrium dynamics is suggested to be a major part of the physiological regulation of GAPDH structure and function. This chapter also contends that a vast amount of useful information can come from comparative analyses of diverse species, particularly regarding protein stability and subunit-subunit interaction. Lastly, the concept of domain exchange is introduced as a means of understanding the stabilization of dynamic oligomers, suggesting that inter-subunit contacts may also be a way of masking docking sites to other proteins.

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Section: Factors Affecting Oligomerizationsupporting

confidence: 89%

Section: Factors Affecting Oligomerizationmentioning

confidence: 99%

Section: Factors Affecting Oligomerizationmentioning

confidence: 99%

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Dynamic Oligomeric Properties

Seidler

2012

GAPDH: Biological Properties and Diversity

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“…A key role of metal species in biological organisms are acting as cofactors of diverse enzymes (Andreini et al 2007). The identified proteins with common metal binding properties, such as the enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been known to function with metal ions as their cofactors (Mounaji et al 2003). GAPDH has been well documented in several previous studies both in plants and algal species (Contreras et al 2010;Li et al 2009;Liu et al 2014;Ritter et al 2010;Song et al 2014;Wang et al 2014;Zou et al 2015), in which this enzyme was found to be up-regulated by Cu stress.…”

Section: Protein Involved In Carbohydrate Metabolismmentioning

confidence: 99%

Leaf proteome characterization in the context of physiological and morphological changes in response to copper stress in sorghum

et al. 2016

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Copper (Cu) is an essential micronutrient required for normal growth and development of plants; however, at elevated concentrations in soil, copper is also generally considered to be one of the most toxic metals to plant cells due to its inhibitory effects against many physiological and biochemical processes. In spite of its potential physiological and economical significance, molecular mechanisms under Cu stress has so far been grossly overlooked in sorghum. To explore the molecular alterations that occur in response to copper stress, the present study was performed in ten-day-old Cu-exposed leaves of sorghum seedlings. The growth characteristics were markedly inhibited, and ionic alterations were prominently observed in the leaves when the seedlings were exposed to different concentrations (0, 100, and 150 µM) of CuSO4. Using two-dimensional gels with silver staining, 643 differentially expressed protein spots (≥1.5-fold) were identified as either significantly increased or reduced in abundance. Of these spots, a total of 24 protein spots (≥1.5-fold) from Cu-exposed sorghum leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Of the 24 differentially expressed proteins from Cu-exposed sorghum leaves, 13 proteins were up-regulated, and 11 proteins were down-regulated. The abundance of most identified protein species, which function in carbohydrate metabolism, stress defense and protein translation, was significantly enhanced, while that of another protein species involved in energy metabolism, photosynthesis and growth and development were severely reduced. The resulting differences in protein expression patterns together with related morpho-physiological processes suggested that these results could help to elucidate plant adaptation to Cu stress and provide insights into the molecular mechanisms of Cu responses in C4 plants.

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“…This has already been shown in a number of aquatic animals exposed to copper, which is known to affect a range of endpoints such as oxygen consumption, blood variables, and energy efficiency (US EPA, 2003), with possible consequences on individual performances. Such effects have been extensively investigated in fish (DeBoeck et al, 1997a, b;Handy et al, 1999;Smith et al, 2001;Campbell et al, 2002;Ali et al, 2003), amphibians (Mounaji et al, 2003;Carattino et al, 2004;Redick and La Point, 2004), and molluscs (Widdows and Johnson, 1988;Belanger et al, 1990;Cheung and Wong, 1998;Elfwing and Tedengren, 2002). Effects of copper on the energetic costs associated with individual performances have also been studied in arthropods.…”

Section: Introductionmentioning

confidence: 99%

Effects of copper on energy metabolism and larval development in the midge Chironomus riparius

Servia

Péry²,

Heydorff

et al. 2006

Ecotoxicology

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When spiked in sediments, copper is known to reduce growth of Chironomus riparius larvae and the production of eggs by adult females. The aim of this work was to better understand the origin of these phenomena by studying the effects of copper using developmental and energetic biomarkers, such as changes in larval weight and age and changes in the levels of sugars and lipids. Four-day-old C. riparius larvae were exposed to nominal concentrations of copper of 0, 6.5, 12.5, 25 and 50 mg/kg of dry sediment (silica) in 0.6 l beakers. They were fed ad libitum and exposures were stopped at 7 and 9 days after the beginning of the tests. The larvae were weighed, sexed and aged. For each sex, the larvae belonging to the phases the most frequently found in the beakers were selected for dissection and measurement of energy reserves. The increase in the concentration of copper resulted in an increasing delay in larval growth in both sexes. Desynchronized development was observed, as shown by the increase in the number of individuals that remained in the third instar or early phases of the fourth instar, as well as by a reduction in age of males. Concerning energy reserves, the levels of sugars (glycogen, trehalose and glucose) in the dissected larvae remained almost constant among levels of exposure. In contrast, at the highest copper concentration (50 mg/kg), triglyceride levels suffered a slight reduction whereas the level of free glycerol significantly increased. It is concluded that selection of C. riparius larvae for both sex and age improves the relevance of some energy-yielding substrates as indicators of adverse physiological effects of copper.

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In vitro effect of metal ions on the activity of two amphibian glyceraldehyde-3-phosphate dehydrogenases: potential metal binding sites

Cited by 19 publications

References 36 publications

Dynamic Oligomeric Properties

Dynamic Oligomeric Properties

Leaf proteome characterization in the context of physiological and morphological changes in response to copper stress in sorghum

Effects of copper on energy metabolism and larval development in the midge Chironomus riparius

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