In vitro effect of tuibur (tobacco brew) on the viability of human blood lymphocytes

Abstract

The use of tobacco and its products are known to cause many illnesses including cancer. A smokeless tobacco locally manufactured called tuibur (tobacco brew) has been consumed by the Mizos from a very long time. In this experiment we aim to determine the cytotoxicity of tuibur by an in vitro study on tuibur-treated human peripheral blood lymphocytes. We have found that 24 h treatment of human lymphocytes with two grades of commercial tuibur and nicotine showed a concentration dependent decrease in cell viability. We, therefore, concluded that as the in vitro use of tuibur has an adverse effect on cell survival, its consumption might have potential side effects on the health of the users.

Determination of Nicotine in Domestic and Imported Cigarettes in Aden (Yemen) Markets

“…The human body obtains nicotine through smoking, chewing and smelling, all of which have dangerous and destructive effects on human health [13]. Consumption of smoking and smokeless tobacco are common all over the world [14]. Long-term tobacco use is associated with increased rates of cancer, chronic obstructive pulmonary disease, atherosclerosis, high blood pressure and low birth weight for babies born to mothers who smoke [15].…”

Nematocidal effects of tobacco infusion (tuibur) against intestinal helminth parasites of chicken

“…Chemical analysis of the tobacco infusion using Fourier transform-infrared spectroscopy has been reported (Lalmuanpuii and Muthukumaran, 2016). Its cytotoxicity is demonstrated against different cancer cell lines such as HeLa, Dalton's lymphoma ascites, Chinese hamster carcinoma cell (V79), as well as cultured human peripheral blood lymphocytes (Lalruatfela et al, 2017). In Mizo traditional medicine, it is used for the treatment of tetanus and as a potent deworming agent against helminth parasites (Sawmliana, 2013).…”