In vitro Effect on Stored Red Blood Cells and Platelets after a 15-Hour Delayed Refrigeration of Whole Blood prior to Component Preparation in CPD-AD

Abstract

We extended the time of keeping whole blood at 20-24 °C to 15 h (overnight) after phlebotomy for preparing platelet concentrates. We have evaluated the in vitro characteristics of platelets and blood cells prepared from whole blood drawn into CPD-AD, an anticoagulant containing 0.4 mM adenine and 1.5 times more dextrose than CPD. We studied in vitro red cell and platelet function of blood cooled either within 4 h after collection or after a 15-hour delay. In vitro platelet function measured as hypotonic shock reaction, aggregation response to ADP and collagen and 14C-serotonin uptake were not significantly different after preparation and after a 5-day storage period. Units held at room temperature for 15 h after blood collection exhibited a level of 2,3-DPG that was 45% of that exhibited by red cells held for 15 h at 1-6°C. All other in vitro parameters of red cell concentrates measured during 35 days of storage were not significantly different. Based on these in vitro data blood drawn into CPD-AD might be kept up to 15 h at room temperature prior to refrigeration in order to prepare platelet concentrates.

Evaluation of the overnight hold of whole blood at room temperature, before component processing: platelets (PLTs) from PLT‐rich plasma

“…However, the relevance of this finding is likely to be small, as the difference was not detectable by Day 5. Our data, comparing 3‐hour versus 24‐hour room temperature hold of WB, confirm and extend earlier reports where no negative effect was seen of a 4‐hour versus 15‐hour room temperature hold of WB on PLT hypotonic shock response, ADP aggregation, collagen aggregation, and 14 C‐serotonin uptake, both immediately after preparation of the PCs and during 5 days of storage 10 . In general, no effects were seen on PLT functionality, measured as ristocetin aggregation and epinephrine/ADP aggregation when comparing 6‐hour versus 24‐hour room temperature hold 5 .…”

Characterization of blood components prepared from whole‐blood donations after a 24‐hour hold with the platelet‐rich plasma method

“…This study presents encouraging results suggesting that WB donations may be stored overnight and processed into blood components with the PRP method with minimal impact on the in vitro measures, mainly of RBC units and cryoprecipate units (FVIII) PLT functions and yields were comparable in PCs processed after either an 8‐ or a 24‐hour hold. Our results showed that PRP preparation can be delayed up to 24 hours after donation, as long as WB is rapidly chilled on cooling plates and maintained at room temperature until processing 1‐3,5‐8,14,15,17‐20,22,33 . With regard to the growing interest to extend PC storage time up to 7 days, 31,34 our results also showed that delaying PLT processing up to 24 hours did not affect their in vitro measures, because no significant differences were observed between 8‐ and 24‐hour components on Day 7.…”

“…Because WB collection often takes place on mobile clinics, the 8‐hour time limit before PRP preparation remains logistically problematic. In 1986, Koerner and coworkers 5 showed that WB might be kept up to 15 hours at room temperature before PC preparation with the PRP method. Pietersz and associates 6 were able to further extend this time limit by demonstrating that the in vitro properties of PLTs and factor VIII (FVIII) content of fresh‐frozen plasma (FFP) were maintained despite an extended storage of WB for 24 hours at room temperature 6 .…”

Evaluation of the properties of components prepared and stored after holding of whole blood units for 8 and 24 hours at ambient temperature

“…The potential for holding WB units at ambient temperature for longer periods after collection was raised in two published studies in the 1980s. One study showed that PLT properties were maintained with a 15‐hour hold time 6 . A second report showed that key in vitro properties for red blood cells (RBCs suspended in SAGM), plasma, and buffy coat–derived PLTs were maintained overnight, essentially a 24‐hour hold 7 .…”