In vitro effects of active constituents and extracts of Orthosiphon stamineus on the activities of three major human cDNA-expressed cytochrome P450 enzymes

In Vivo Effect of Ruta Chalepensis Extract on Hepatic Cytochrome 3a1 in Rats

“…Previous research has reported on the assessment of different solvents used to obtain R. chalepensis extract; its authors have concluded that the aqueous extract contains the greatest amount of constituents and cancels the risk of ethanol, hexane, or another type of solvents interacting with the plant's constituents, which cause an effect on the cytochromes under study (Nazish et al, 2009). This is a possible explanation to the results found by Pan et al (2011), who concluded that the petroleum ether (PE) extract of Orthosiphon stamineus produces the greatest inhibitor effect on CYP2C19. At the time this experiment was published, there were no other studies on R. chalepensis extract in cytochromes available to compare our results; however, previous research reports on the study of other plants, such as Andrographis paniculata (Pekthong et al, 2009), Orthosiphon stamineus, Aframomum cuspidatum (Agbonon et al, 2010), and Centella asiatica (Pan et al, 2011) in cell culture.…”

“…This is a possible explanation to the results found by Pan et al (2011), who concluded that the petroleum ether (PE) extract of Orthosiphon stamineus produces the greatest inhibitor effect on CYP2C19. At the time this experiment was published, there were no other studies on R. chalepensis extract in cytochromes available to compare our results; however, previous research reports on the study of other plants, such as Andrographis paniculata (Pekthong et al, 2009), Orthosiphon stamineus, Aframomum cuspidatum (Agbonon et al, 2010), and Centella asiatica (Pan et al, 2011) in cell culture. Centella asiatica was also studied in vivo by Kulthong et al, (2009).…”

“…These enzymes are the main mechanism for increased, diminished, or null bioavailability of herbal-based drugs and compounds when said enzymes and drugs are simultaneously administered (Pekthong et al, 2009;Wang et al, 2009). They are termed P450 because an absorbance peak is produced at 450 nm when treated with a reducer agent and linked with carbon monoxide (Pan et al, 2011). In rats, the CYP450 enzymes commonly involved in the metabolism of xenobiotics include CYP1a2, CYP2c11, CYP2e1, and CYP3a1/2 (Li et al, 2008), while in humans, the enzymes involved are CYP1A2, CYP2C9, CYP2D6, and CYP3A4 (Ponnusankar et al, 2011;Zhou et al, 2011).…”

Inhibitory effects of gypenosides on seven human cytochrome P450 enzymes in vitro

“…This IC 50 unit could be compared to physiological distribution volumes and allows for a uniform scale to assess the inhibitory potential of herbal components in relation to physiologically relevant parameters (Pan et al, 2011). Our calculation was based on one gypenosides product (total Jiaogulan saponin tablets, each tablet contains 20 mg gypenosides, Manufacturer: Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd.) which was available from local market and it was of commercial quality.…”

Virtual Searching of Dummy Template for Sinensetin Based on 2d Molecular Similarity Using Chemdb Tool

“…Akowuah et al has separated sinensetin in the microscale using high performance thin layer chromatography (HPTLC) for quantitative determination [2]. For macroscale separation, especially to produce sinensetin as herb product, solvent extraction method is used [3]. If necessary, it needs special techniques such as sonication [1] or microwave-assisted extraction [4].…”