In vitro effects of dental monomer exposure - Dependence on the cell culture model

Morisbak, Else; Uvsløkk, Solveig; Samuelsen, Jan Tore

doi:10.1016/j.tiv.2020.104906

Cited by 6 publications

(12 citation statements)

References 41 publications

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“…Acryloyl chloride was freshly distilled before use. 1 H and 13 C NMR spectra were acquired with a Bruker AMX (400 and 100 MHz, respectively). 1 H NMR chemical shifts are reported as values in ppm relative to CHCl 3 (7.26) in CDCl 3 , H 2 O (4.79) in D 2 O and Me 4 Si (TMS) was used as internal standard.…”

Section: Monomers Synthesismentioning

confidence: 99%

“…1 H NMR (400 MHz, Deuterium Oxide) δ 6.81 -6.60 (m, 2H), 6.21 -6.06 (m, 2H), 5.86 -5.70 (m, 2H), 3.56 -3.34 (m, 4H), 3.11 (s, 3H), 2.97 (s, 3H), 2.48 -2.30 (m, 3H), 2.28 -2.12 (m, 3H), 1.88 -1.67 (m, 4H). 13 C NMR (101 MHz, Deuterium Oxide) δ 169. 01, 168.77, 128.36, 128.28, 127.70, 127.40, 53.56, 53.50, 53.17, 53.10, 48.39, 46.28, 46.24, 40.90, 40.82, 35.59, 33.91, 24.74, 24.72, 23.40, 23.34 13 C spectra were observed suggesting multiple isomeric forms due to the ring structure of BAADA (Figure S3).…”

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

“…13 C NMR (101 MHz, Deuterium Oxide) δ 169. 01, 168.77, 128.36, 128.28, 127.70, 127.40, 53.56, 53.50, 53.17, 53.10, 48.39, 46.28, 46.24, 40.90, 40.82, 35.59, 33.91, 24.74, 24.72, 23.40, 23.34 13 C spectra were observed suggesting multiple isomeric forms due to the ring structure of BAADA (Figure S3).…”

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

“…The synthesis procedure was the same as described above, with an additional quaternization step which can be found in supplementary information. 1 H spectra and duplicate peaks in the 13 C spectra are shown in Figures S4-6.…”

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

“…Additionally, the copolymerization of HEMA with a hydrophobic difunctional methacrylate (such as BisGMA and UDMA) forms a loosely cross-linked network, with hydrophilic HEMA-rich domains [12] that are easily plasticized by swelling with water. Also, due to its low molecular weight and the tendency to partition in water, HEMA is easily extracted from the polymer network, with consequent biological repercussions, such as suppression of expression of certain genes associated with protection against excessive cell proliferation, apoptosis and cell mutation [13]. Other than HEMA, the dimethacrylates, commonly used as base monomers can also pose concern in terms of cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of di- and triacrylamides with tertiary amine cores and their evaluation as monomers in dental adhesive interfaces

et al. 2020

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Purpose/aim: In an attempt to increase the service life of dental adhesive interfaces, more hydrolytically and enzymatically-stable methacrylate alternatives, such as methacrylamides, have been proposed. The aim of this study was to investigate polymerization behavior, as well as mechanical and biological properties of experimental adhesives containing multi-functional acrylamides. ethyl]amine -TMAAEA, N,N'-bis(acrylamido) 1,4-diazepane -BAADA, N,N-Diethyl-1,3bis(acrylamido)propane -DEBAAP) or HEMA (2-Hydroxyethyl methacrylate -control) were added at 40 wt% to UDMA. 0.2 wt% DMPA and 0.4 wt% DPI-PF6 were used as initiators. Polymerization kinetics was followed in real-time in near-IR during photoactivation (320-500 nm, at 630 mW/cm 2 ). Water sorption/solubility and flexural strength/modulus were measured according to ISO 4049. 1 H NMR was used to assess monomer degradation kinetics. MTT assay was used to assess cytotoxicity against OD-21 and DPSC cells. Biofilm formation and adhesion were assessed by Luciferase Assay and Impingement technique, respectively. Solvated adhesives (40 vol% ethanol) were used to test interfacial adhesion strength. The results were analyzed by ANOVA/Tukey's test (α = 0.05). Materials and methods: Results:In general, the pure methacrylate mixture had higher rate of polymerization (Rp max ), degree of conversion (DC) at Rp max , and final DC than the acrylamides. Flexural properties after

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Section: Monomers Synthesismentioning

confidence: 99%

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

Section: Nn-bis[(3-methylaminoacryl)propyl]methylamine (Bmaapma)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of di- and triacrylamides with tertiary amine cores and their evaluation as monomers in dental adhesive interfaces

et al. 2020

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Digital manufacturing techniques and the in vitro biocompatibility of acrylic-based occlusal device materials

Haugli,

Alkarra,

Samuelsen

2024

Clin Oral Invest

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Objectives Material chemistry and workflow variables associated with the fabrication of dental devices may affect the biocompatibility of the dental devices. The purpose of this study was to compare digital and conventional workflow procedures in the manufacturing of acrylic-based occlusal devices by assessing the cytotoxic potential of leakage products. Methods Specimens were manufactured by 3D printing (stereolithography and digital light processing), milling, and autopolymerization. Print specimens were also subjected to different post-curing methods. To assess biocompatibility, a human tongue epithelial cell line was exposed to material-based extracts. Cell viability was measured by MTT assay while Western blot assessed the expression level of selected cytoprotective proteins. Results Extracts from the Splint 2.0 material printed with DLP technology and post-cured with the Asiga Flash showed the clearest loss of cell viability. The milled and autopolymerized materials also showed a significant reduction in cell viability. However, by storing the autopolymerized material in dH2O for 12 h, no significant viability loss was observed. Increased levels of cytoprotective proteins were seen in cells exposed to extracts from the print materials and the autopolymerized material. Similarly to the effect on viability loss, storing the autopolymerized material in dH2O for 12 h reduced this effect. Conclusions/Clinical relevance Based on the biocompatibility assessments, clinical outcomes of acrylic-based occlusal device materials may be affected by the choice of manufacturing technique and workflow procedures.

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Thermo-responsive polymers as surface active compounds: A review

Manfredini,

Gardoni,

Sponchioni

et al. 2023

European Polymer Journal

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In vitro effects of dental monomer exposure - Dependence on the cell culture model

Cited by 6 publications

References 41 publications

Synthesis of di- and triacrylamides with tertiary amine cores and their evaluation as monomers in dental adhesive interfaces

Synthesis of di- and triacrylamides with tertiary amine cores and their evaluation as monomers in dental adhesive interfaces

Digital manufacturing techniques and the in vitro biocompatibility of acrylic-based occlusal device materials

Thermo-responsive polymers as surface active compounds: A review

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