In vitro effects of IL-12 and IL-2 on NK cells, cytokine release and clonogenic activity in myelodysplastic syndromes (MDS)

Abstract

We evaluated the in vitro effects of IL-12, alone and in associIn this study, we investigated the effect of IL-12, alone and ation with IL-2 on MDS bone marrow and peripheral blood cells.in association with IL-2, on PB and BM NK cells, lymphocyte Thirty-six patients and 14 healthy subjects were studied. Natuphenotype, cytokine release and BM clonogenic activity in being affected by refractory anemia (RA n = 10), refractory

In vitro IL-12 treatment of peripheral blood mononuclear cells from patients with leukemia or myelodysplastic syndromes: increase in cytotoxicity and reduction in WT1 gene expression

“…In vitro studies have demonstrated that IL-12 can enhance the non-MHCrestricted cytolytic activity of NK cells 3 and facilitate specific allogeneic human cytolytic T lymphocyte responses against fresh leukemia cells and cell lines. [4][5][6] IL-12 can potently induce IFN-␥, 7,8 and up-regulate the expression of cytolytic components including perforin, serine esterase and TIA-1 in peripheral blood lymphocytes. 9 IL-12 can also stimulate continuous proliferation of both T lymphocytes and NK cells.…”