In vitro effects of N-acetylcysteine alone and combined with tigecycline on planktonic cells and biofilms of Acinetobacter baumannii

Feng, Jinlun; Liu, Baomo; Xu, Jin; Wang, Qinqin; Huang, Lixia; Ou, Weijun; Gu, Jincui; Wu, Jianyong; Li, Shaoli; Zhuo, Chao; Zhou, Yan

doi:10.21037/jtd.2017.11.130

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…It has yet to be determined if variability in biofilm production affects treatment or clinical outcome. Although several studies have reported on biofilm treatment agents, they have not specified differences in efficacy of agents on isolates that have produced high, moderate or low biofilm production . This is an area that future studies may clarify.…”

Section: Discussionmentioning

confidence: 99%

Biofilm production of Pseudomonas spp. isolates from canine otitis in three different enrichment broths

Robinson¹,

Paterson²,

Bennett³

et al. 2019

Veterinary Dermatology

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Background -Pseudomonas spp. are commonly isolated from dogs with clinical otitis and have been shown to produce biofilm. There is a paucity of studies demonstrating biofilm growth in veterinary medicine.Hypothesis/objectives -To compare biofilm production of Pseudomonas spp. isolated from dogs with otitis using three different enrichment broths at two different time points. Speciation was performed.Animals -One hundred isolates from 98 dogs with clinical otitis were assessed for biofilm production.Methods and materials -One hundred isolates were assessed for biofilm production using a microtitre plate assay. Biofilm production in Luria-Bertani Broth (LBB), Mueller-Hinton Broth (MHB) and Tryptic Soy Broth (TSB) were assessed after 18 and 24 h of incubation.Results -At 18 h, biofilm production was demonstrated in 87% of LBB, 91% of TSB and 93% of MHB grown isolates. By 24 h, this was 92% of LBB, 96% of TSB and 99% of MHB isolates. Biofilm production was significantly increased after 24 h incubation compared to 18 h. A significant difference was noted in biofilm production between LBB and MHB (P = 0.0349), but not between LBB and TSB (P = 0.3727) or MHB and TSB (P = 0.3687) at 24 h incubation. Two isolates were speciated as P. fluorescens and 98 as P. aeruginosa.Conclusion and clinical importance -Not all enrichment broths were equivalent to one another and 24 h incubation was superior to 18 h. Biofilm production was high in this population of Pseudomonas spp. isolates.

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Section: Discussionmentioning

confidence: 99%

Biofilm production of Pseudomonas spp. isolates from canine otitis in three different enrichment broths

Robinson¹,

Paterson²,

Bennett³

et al. 2019

Veterinary Dermatology

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“…Due to its mucolytic activity, NAC has attracted microbiologists to explore its potential as an antibacterial and antibiofilm agent. A number of in vitro studies assessed the activity of NAC against different Gram positive and Gram-negative bacteria including Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Staphylococcus epidermidis amongst others (Quah et al,2012; Choi et al,2018; Feng et al,2018; Jun et al,2019; Ciacci et al, 2019; Li et al,2020; Guerini et al,2020; Kundukad et al, 2020; Manoharan et al, 2021). Given the broad-spectrum activity of NAC against bacteria, this study aims at repurposing N-acetyl cysteine (NAC) to treat biofilms of XDR K. pneumoniae , isolated from patients in hospitals.…”

Section: Introductionmentioning

confidence: 99%

Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Chauhan

Bhowmik

Rohit

2022

Preprint

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Klebsiella pneumoniae is one of the leading causes of community and nosocomial infections. Reduced treatment options against extensively drug resistant (XDR) - K. pneumoniae, is a serious concern in hospital settings, and hence, WHO has categorized it as a critical priority pathogen. Biofilm forming ability is a common virulence mechanism amongst K. pneumoniae that is associated with antibiotic tolerance up to 1000X MIC and hence, are difficult to treat. N-acetyl cysteine (NAC) is an FDA approved mucolytic drug used to treat acetaminophen-associated toxicity and obstructive pulmonary diseases. In this study, we assessed antibacterial and antibiofilm activity of NAC against clinical isolates of XDR K. pneumoniae, obtained from Madras Medical Mission Hospital, India. To assess the biofilm eradication ability of NAC, we grew biofilms in 96 well plates and treated the mature biofilms with different concentrations of NAC. We observed that the biofilms of only 3 isolates of XDR K. pneumoniae could be eradicated at a concentration as low as 20mg/ml. Although increasing the concentration of NAC to 80mg/mL could significantly reduce the biofilms of all the isolates up to 4-5 Log, NAC at a concentration of 100 mg/mL successfully eradicated the mature biofilms of all the isolates of XDR K. pneumoniae. This in vitro study demonstrates the potential of NAC as an efficient agent against the biofilms of clinical isolates of XDR-K. pneumoniae and thus provides a promising alternative to antibiotics.

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Investigating Biofilm Formation and Antibiofilm Activity Using Real Time Cell Analysis Method in Carbapenem Resistant Acinetobacter baumannii Strains

Temel

Eraç

2022

Curr Microbiol

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In vitro effects of N-acetylcysteine alone and combined with tigecycline on planktonic cells and biofilms of Acinetobacter baumannii

Abstract: NAC alone significantly inhibited biofilm formation of . The combination of NAC and TGC induced partial synergistic effect against planktonic cells and synergistic effect against biofilm-embedded, which might be a therapeutic option for biofilm-related infections of .

Cited by 11 publications

References 31 publications

Biofilm production of Pseudomonas spp. isolates from canine otitis in three different enrichment broths

Biofilm production of Pseudomonas spp. isolates from canine otitis in three different enrichment broths

Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Investigating Biofilm Formation and Antibiofilm Activity Using Real Time Cell Analysis Method in Carbapenem Resistant Acinetobacter baumannii Strains

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