In Vitro Effects of Titanium Dioxide Nanoparticles (TiO2NPs) on Cadmium Chloride (CdCl2) Genotoxicity in Human Sperm Cells

Santonastaso, Marianna; Mottola, Filomena; Iovine, Concetta; Cesaroni, Fulvio; Colacurci, Nicola; Rocco, Lucia

doi:10.3390/nano10061118

Cited by 34 publications

(25 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nano-TiO 2 can cause genotoxicity [ 41 ]. Furthermore, the ROS-mediated stress within cells could be the main mechanism for the genotoxicity of nano-TiO 2 [ 42 ]. Further studies are needed to explore whether the TiO 2 NPs and NTs can jointly cause DNA damage due to the production of ROS.…”

Section: Resultsmentioning

confidence: 99%

Combined Toxicity of TiO2 Nanospherical Particles and TiO2 Nanotubes to Two Microalgae with Different Morphology

et al. 2020

View full text Add to dashboard Cite

The joint activity of multiple engineered nanoparticles (ENPs) has attracted much attention in recent years. Many previous studies have focused on the combined toxicity of different ENPs with nanostructures of the same dimension. However, the mixture toxicity of multiple ENPs with different dimensions is much less understood. Herein, we investigated the toxicity of the binary mixture of TiO2 nanospherical particles (NPs) and TiO2 nanotubes (NTs) to two freshwater algae with different morphology, namely, Scenedesmus obliquus and Chlorella pyrenoidosa. The physicochemical properties, dispersion stability, and the generation of reactive oxygen species (ROS) were determined in the single and binary systems. Classical approaches to assessing mixture toxicity were applied to evaluate and predict the toxicity of the binary mixtures. The results show that the combined toxicity of TiO2 NPs and NTs to S. obliquus was between the single toxicity of TiO2 NTs and NPs, while the combined toxicity to C. pyrenoidosa was higher than their single toxicity. Moreover, the toxicity of the binary mixtures to C. pyrenoidosa was higher than that to S. obliquus. A toxic unit assessment showed that the effects of TiO2 NPs and NTs were additive to the algae. The combined toxicity to S. obliquus and C. pyrenoidosa can be effectively predicted by the concentration addition model and the independent action model, respectively. The mechanism of the toxicity caused by the binary mixtures of TiO2 NPs and NTs may be associated with the dispersion stability of the nanoparticles in aquatic media and the ROS-induced oxidative stress effects. Our results may offer a new insight into evaluating and predicting the combined toxicological effects of ENPs with different dimensions and of probing the mechanisms involved in their joint toxicity.

show abstract

Section: Resultsmentioning

confidence: 99%

Combined Toxicity of TiO2 Nanospherical Particles and TiO2 Nanotubes to Two Microalgae with Different Morphology

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Hence, our study investigated the potentially antigenotoxic functions of EA, an antioxidant compound poorly studied in seminal fluid. In this study, human sperm cells were exposed in vitro to EA to investigate its potential in contrasting the genotoxic effect of benzene (Santonastaso et al, 2020). First, we observed that sperm DNA integrity was not affected by EA incubation, regardless the length of exposure.…”

Section: Discussionmentioning

confidence: 99%

“…The polymorphic pattern generated by the RAPD‐PCR profiles allowed the calculation of the genomic template stability (GTS%) as follows: GTS% = (1 − a/ n ) × 100, where a is the average number of polymorphic bands detected in each exposed sample, and n corresponds to the number of total bands in the nontreated cells. The template genomic stability of control was set to 100% (Santonastaso et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Considering the strong anti‐inflammatory and antioxidant properties, and the role that antioxidants play in preventing ROS‐induced damage in the male reproductive system, the aim of this study was to evaluate the capacity of EA to improve sperm parameters and reduce DNA damage after in vitro exposure of human sperm to benzene (45, 60, and 90 min). Benzene was chosen as it is a toxic environmental compound able affecting human sperm motility and vitality as well as inducing DNA fragmentation (Noel & Rath, 2006; Ollero et al, 2001; Rocco et al, 2014; Santonastaso et al, 2019; Santonastaso et al, 2020; WHO, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa

Iovine

Mottola

Santonastaso

et al. 2021

Molecular Reproduction Devel

Self Cite

View full text Add to dashboard Cite

Oxidative stress (OS) plays a significant role in the etiology of male infertility, resulting in the impairment of male reproduction. This condition, characterized by an imbalance in the levels of oxidizing and antioxidant species in the seminal fluid, has a harmful impact on sperm functions and DNA integrity. The present study aimed to evaluate the anti‐genotoxic action of ellagic acid, a polyphenolic molecule of natural origin having a powerful antigenotoxic, anti‐inflammatory and antiproliferative role. An OS condition was induced in vitro by incubating normozoospermic human semen samples in benzene for 45, 60 and 90 min. DNA integrity was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay, RAPD‐PCR was performed to calculate the genome template stability, while the percentage of intracellular reactive oxygen species (ROS) was assessed by the 2ʹ, 7ʹ‐dichlorofluorescein assay. Our results showed that ellagic acid has a consistent protective effect on DNA integrity, as well as on sperm vitality and motility, by counteracting generation of intracellular ROS. The results of this study suggest ellagic acid as a suitable molecule to protect sperm DNA from oxidative stress, with a potentially significant translational impact on the management of the male infertility.

show abstract

“…Exposure to cadmium causes destructive effects in male and female reproductive systems and negatively affects on sperm parameters (Kumar & Sharma, 2019; Pollack, Ranasinghe, Sjaarda, & Mumford, 2014; Zhao et al, 2017). Previous studies showed that cadmium induces DNA fragmentation during apoptosis in various cells in vitro and in vivo (Acosta et al, 2016; El‐Atta et al, 2014; Santonastaso et al, 2020). Also, it was emphasised that cadmium exerts its toxic effects through induction of oxidative stress (Xia, Chen, Dahms, Ying, & Peng, 2016).…”

Section: Introductionmentioning

confidence: 98%

Impact of silymarin on cadmium‐induced apoptosis in human spermatozoa

2020

View full text Add to dashboard Cite

Oxidative stress‐induced apoptosis in spermatozoa may lead to male infertility. Environmental pollutants and heavy metals such as cadmium cause harmful effects on the reproductive system and sperm parameters through the induction of oxidative stress. Silymarin, as a potent antioxidant, is able to inhibit oxidative stress. This study was performed to investigate the protective effects of silymarin on cadmium‐induced toxicity in human spermatozoa. Sperm samples were divided into the following five groups: (a) spermatozoa at 0 min, (b) spermatozoa in the control group, (c) spermatozoa treated with cadmium chloride (20 μM), (d) spermatozoa treated with silymarin (2 μM)+ cadmium chloride (20 μM) and (e) spermatozoa treated with silymarin (2 μM). Sperm parameters related to apoptosis, such as DNA fragmentation, nucleus diameter, mitochondrial membrane potential (MMP) and expression of caspase‐3, were evaluated in all groups. After 180 min, spermatozoa treated with cadmium chloride showed a significant decrease in nucleus diameter and MMP but a significant increase in DNA fragmentation; however, caspase‐3 expression remained unchanged. At this time point, silymarin in the silymarin + cadmium chloride group could significantly reverse the adverse effects of cadmium chloride on these parameters.Silymarn could partly compensate for the caspase‐independent apoptosis in the spermatozoa. Therefore, oxidative stress could be a consequence for cadmium toxicity.

show abstract

In Vitro Effects of Titanium Dioxide Nanoparticles (TiO2NPs) on Cadmium Chloride (CdCl2) Genotoxicity in Human Sperm Cells

Cited by 34 publications

References 76 publications

Combined Toxicity of TiO2 Nanospherical Particles and TiO2 Nanotubes to Two Microalgae with Different Morphology

Combined Toxicity of TiO2 Nanospherical Particles and TiO2 Nanotubes to Two Microalgae with Different Morphology

In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa

Impact of silymarin on cadmium‐induced apoptosis in human spermatozoa

Contact Info

Product

Resources

About