In vitro electro-mechanical characterization of human knee articular cartilage of different degeneration levels: A comparison with ICRS and Mankin scores

Abedian, Reza; Willbold, Elmar; Hurschler, Christof

doi:10.1016/j.jbiomech.2013.02.004

Cited by 26 publications

(27 citation statements)

References 33 publications

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“…These color maps agree with previously reported contrast agent distribution patterns for CA4+ [10, 31, 32] and for Ioxaglate [17, 21, 32]. Additionally, the Safranin-O stain distribution in the histological sections agrees with previous reports for human cartilage [35, 36]. Thus, CA4+ enhanced CT attenuation reflects GAG distribution within human metacarpal cartilage tissue.…”

Section: Discussionsupporting

confidence: 91%

Contrast-enhanced CT facilitates rapid, non-destructive assessment of cartilage and bone properties of the human metacarpal

Lakin

Ellis

Shelofsky

et al. 2015

Osteoarthritis and Cartilage

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Objective The aim of this work is to establish the human metacarpal as a new whole joint surface early-stage osteoarthritis (OA) model that enables comparisons of articular cartilage and subchondral bone through high resolution contrast-enhanced CT (CECT) imaging, mechanical testing, and biochemical analysis. Design The 4th metacarpal was obtained from 12 human cadaveric donors and baseline μCT imaging was followed by indentation testing. The samples were then immersed in anionic (Ioxaglate) and cationic (CA4+) iodinated contrast agent solutions followed by CECT. Cartilage GAG content and distribution was measured using the 1,9 dimethylmethylene blue (DMMB) assay and Safranin-O histology staining. Linear regression was performed to compare cartilage and subchondral bone properties. Results Strong and significant positive correlations were observed between CA4+ CECT attenuation and both GAG content (R2=0.86) and equilibrium modulus (R2=0.84), while correlations using Ioxaglate were insignificant (R2≤0.24, p>0.05). Subchondral bone plate (SBP) thickness negatively and significantly correlated with SBP mineral density (R2=0.49). Cartilage GAG content significantly correlated with several trabecular bone properties, including positive correlations with bone volume fraction (%BV/TV, R2=0.67), trabecular number (Tb.N, R2=0.60), and trabecular thickness (R2=0.42), and negative relationships with structural model index (SMI, R2=0.78) and trabecular spacing (Tb.Sp, R2=0.56). Similarly, equilibrium modulus correlated positively with %BV/TV (R2=0.50), Tb.N (R2=0.59) and negatively with Tb.Sp (R2=0.55) and SMI (R2=0.60). Conclusion This study establishes the human metacarpal as a new early-stage OA model suitable for rapid, high resolution CECT imaging, mechanical testing, and biochemical analysis of the cartilage and subchondral bone, and for examining their inter-relationships.

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Section: Discussionsupporting

confidence: 91%

Contrast-enhanced CT facilitates rapid, non-destructive assessment of cartilage and bone properties of the human metacarpal

Lakin

Ellis

Shelofsky

et al. 2015

Osteoarthritis and Cartilage

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“…Indeed, electromechanical QP and subchondral-bone plate thickness increased remarkably with degeneration, while cartilage thickness barely changed. These observations agree with previously published studies where the measured streaming potentials varied according to different early degeneration stages of cartilage 19,24,28 . Effectively, since cartilage alteration is characterized by enzymatically cleaved collagen network and a depletion of proteoglycan content e which leads to a biomechanical weakening of the tissue e lower streaming potentials are then generated during compression 24,26 .…”

Section: Discussionsupporting

confidence: 93%

“…Cartilage electromechanical properties were measured using a hand-held probe (Benchtop Arthro-BST, Biomomentum) 19,25,27,28 . This device allows a non-destructive measurement of streaming potentials generated during a light indentation (~1 s) of the articular cartilage with an array of 37 gold microelectrodes lying on a semi-spherical indenter (Ø ¼ 6.35 mm, 5 microelectrodes/mm 2 ).…”

Section: Electromechanical Assessmentmentioning

confidence: 99%

Electromechanical properties of human osteoarthritic and asymptomatic articular cartilage are sensitive and early detectors of degeneration

Hadjab

Sim

Karhula

et al. 2018

Osteoarthritis and Cartilage

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“…Moreover, the role of the camera‐registration system accompanying both systems is crucial since it registers the location of test measurements which in the case of the electromechanical probe allows for real‐time positioning and, in the case of the automated indentation technique, allows for mechanically controlled positioning over the surface. For clinical evaluation of cartilage, the electromechanical probe offers a major advantage over the automated indentation technique since it can be used as an arthroscopic tool and has already shown reliability in assessing the quality of cartilage and user‐independence . Nonetheless, the automated indentation technique has the advantage of being used on animals as small as rabbit or mice for in vitro studies …”

Section: Discussionmentioning

confidence: 99%

“…For clinical evaluation of cartilage, the electromechanical probe offers a major advantage over the automated indentation technique since it can be used as an arthroscopic tool and has already shown reliability in assessing the quality of cartilage and user-independence. 15,16,[41][42][43] Nonetheless, the automated indentation technique has the advantage of being used on animals as small as rabbit or mice for in vitro studies. 44 A limitation imposed by our study design is that our sensitivity analysis was based on macroscopic visual assessment (assumed comparable to the arthroscopic assessment) for delimiting all regions.…”

Section: Cartilage Electromechanical and Indentation Assessmentsmentioning

confidence: 99%

Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

Sim

Chevrier

Garon

et al. 2016

Journal Orthopaedic Research

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Recent advances in the development of new drugs to halt or even reverse the progression of Osteoarthritis at an early-stage requires new tools to detect early degeneration of articular cartilage. We investigated the ability of an electromechanical probe and an automated indentation technique to characterize entire human articular surfaces for rapid non-destructive discrimination between early degenerated and healthy articular cartilage. Human cadaveric asymptomatic articular surfaces (four pairs of distal femurs and four pairs of tibial plateaus) were used. They were assessed ex vivo: macroscopically, electromechanically, (maps of the electromechanical quantitative parameter, QP, reflecting streaming potentials), mechanically (maps of the instantaneous modulus, IM), and through cartilage thickness. Osteochondral cores were also harvested from healthy and degenerated regions for histological assessment, biochemical analyses, and unconfined compression tests. The macroscopic visual assessment delimited three distinct regions on each articular surface: Region I was macroscopically degenerated, region II was macroscopically normal but adjacent to regions I and III was the remaining normal articular surface. Thus, each extracted core was assigned to one of the three regions. A mixed effect model revealed that only the QP (p < 0.0001) and IM (p < 0.0001) were able to statistically discriminate the three regions. Effect size was higher for QP and IM than other assessments, indicating greater sensitivity to distinguish early degeneration of cartilage. When considering the mapping feature of the QP and IM techniques, it also revealed bilateral symmetry in a moderately similar distribution pattern between bilateral joints. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:858-867, 2017.

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In vitro electro-mechanical characterization of human knee articular cartilage of different degeneration levels: A comparison with ICRS and Mankin scores

Cited by 26 publications

References 33 publications

Contrast-enhanced CT facilitates rapid, non-destructive assessment of cartilage and bone properties of the human metacarpal

Contrast-enhanced CT facilitates rapid, non-destructive assessment of cartilage and bone properties of the human metacarpal

Electromechanical properties of human osteoarthritic and asymptomatic articular cartilage are sensitive and early detectors of degeneration

Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage

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