In Vitro Embryo Culture of Some Sweet Cherry Genotypes

Asănică, A.; Tudor, V.; Plopa, Catița; Sumedrea, Mihaela; Peticilă, A.; Teodorescu, Răzvan; Tudor, Valentina

doi:10.1016/j.aaspro.2016.09.049

Cited by 5 publications

(6 citation statements)

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“…The escalating threat of extreme weather events caused by climate change is set to pose an increasingly serious challenge to goji berry production, major threats being extreme drought and insolation [62,63]. The Romanian homologated varieties were developed based on Chinese varieties germplasm, due to their high fruit quality traits [64,65], without using the local germplasm.…”

Section: Introductionmentioning

confidence: 99%

Exploring Genetic Diversity in Romanian Lycium Species: A Comparative Study of Wild and Cultivated Genotypes

Ciceoi,

Asănică,

Luchian

et al. 2023

Preprint

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Goji berries, long valued in Chinese medicine and cuisine for their wide range of medicinal benefits, are considered a 'superfruit' and functional food. Out of the nearly 100 Lycium species known for their genetic diversity, L. barbarum and L. chinense currently dominate the market. Due to increasing market demand and concerns about food safety and sustainability, Europe and the Americas are expanding the local goji berry production, using as starting material plants originated from China. European breeding programs are focusing on Lycium to develop varieties adapted to local conditions, especially in response to climate change. By 2023, Romania registered seven goji berry varieties, both from L. barbarum and L. chinense species, without incorporating local germplasm. Advanced genomic studies, including NGS sequencing of five cultivated and three wild goji berry plant genomes, have been conducted to analyse their genetic variation, with a particular focus on the BODYGUARD 3 and 4 genes. This research uncovered significant differences between cultivated and wild genotypes, both in the entire genome and specifically in the BODYGUARD genes, providing crucial insights for goji berry breeders to support the development of goji berry cultivation in Romania.

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Section: Introductionmentioning

confidence: 99%

Exploring Genetic Diversity in Romanian Lycium Species: A Comparative Study of Wild and Cultivated Genotypes

Ciceoi,

Asănică,

Luchian

et al. 2023

Preprint

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“…Современная биотехнология растений этого направления пополняется новыми знаниями о составе применяемых искусственных питательных сред (Zdruikovskaya-Richter, 1964;Plaksina et al, 2013;Kukharchik et al, 2006;Asanica et al, 2016) и обеззараживающих препаратов, позволяющих снизить уровень инфицированности зародышей (Podorozhnyi, 1998;Plaksina, 2016). При всей значимости вышеприведенных задач, решаемых биотехнологами в процессе работы, выход селекционного материала оказывается невысоким.…”

Section: Introductionunclassified

“…Чеботарева считает, что для культивирования зародышей вишни наиболее благоприятным является срок их изоляции на ранней стадии развития -на 19-22-й день, к этому периоду относят стадии развития зародыша «сердечко» и «торпеда» (Chebotareva, 1990). Зарубежные ученые, работая с культурой зародышей сортов черешни, показали, что зародыши успешно поддаются культивированию при их вычленении из недозрелых плодов «желто-зеленой» стадии (Kukharchik et al, 2006;Woo, Wetzstein, 2008;Dulic et al, 2016;Asanica et al, 2016).…”

Section: Introductionunclassified

Effect of the timing of embryo isolation on the output of seedlings of sour cherry varieties when cultivated <i>in vitro</i>

Kovalenko

Поливара

2020

Biotehnologiâ i selekciâ rastenij

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The breeders are faced with the task of the fastest breeding of sour cherry (Prunus cerasus L) varieties, which will be able to fill a niche among the insurance stone fruit crops, complementing the existing assortment. It is practically impossible to speed up this process without the use of in vitro culture. In this regard, the purpose of the present study was to identify the most favorable timing for isolating embryos of sour cherry varieties and hybrids with sweet cherry for embryos active development and growth in vitro. Analysis of the literature data and the results obtained in this study allows a conclusion that the timing of embryos isolation for their introduction into in vitro culture plays a very important role for the entire breeding process. Due to the discrepancy in defining the timing, a certain starting point is proposed, which is the date of fertilization (artificial pollination), or mass flowering in the case of free pollination. The parameters (height and width) of sour cherry seeds, which depend on the period of pollination, are given. Together with the cotyledons, the embryos of sour cherry varieties on the 14th day of their development correspond to sizes from 1.2 × 0.4 mm to 1.6 × 1.0 mm and, in the case of normal fertilization, increase in size from 6 .5 × 5.1 mm to 7.2 × 6.3 mm by day 28-30, on an average. At the same time, the seed with the embryo of hybrid sour cherry varieties (dukes) reaches higher values, e.g., 11.3 × 8.1 mm. When introducing cherry embryos into culture, one should focus on their size, as well as on the period that has passed from pollination. It was found that the periods of isolation, both early on day 14-19 and late on day 44-52, negatively affect the development of embryos: they stop their development, and the number of the obtained seedlings decreases. It has been experimentally proven that the most optimal timing of fruit picking for growing cherry hybrids in vitro is the period from day 28 to 32 after pollination of flowers: active development was observed in them on days 10-15 in test tubes, and most of them (83-85%) began to germinate 40 days later (on day 39-47 after the introduction into in vitro culture). In general, this leads to the efficient hybridization and accelerated obtaining of seedlings in the same year.

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“…Метод культивирования зародышей in vitro уже несколько десятилетий занимает важное место при разработке различных селекционных программ по черешне в России (Здруйковская-Рихтер, 1964; Кухарчик, 1994; Ковальчук, Чуканова, 1997; Захарченко, Бунцевич, 2001; Коваленко, 2017) и за рубежом (Balla, Brozik, 1996;Stanys, 1998;Standardi, 2012;Asănică et al, 2016). Из анализа литературных данных следует, что на культуру эмбрионов влияют прежде всего генотип и искусственная питательная среда.…”

Section: Introductionunclassified

“…Из анализа литературных данных следует, что на культуру эмбрионов влияют прежде всего генотип и искусственная питательная среда. Cреды при культивировании зародышей составлялись преимущественно зарубежными авторами на основе солей питательных сред Мурасиге и Скуга (Asănică et al, 2016;Dulić et al, 2016). Использовались также варианты питательных сред, созданные на основе состава, предложенного De Fossard (1977).…”

Section: Introductionunclassified

In vitro cultivation of the embryos of hybrid forms of early-ripening sweet cherry (Prunus avium L.) varieties

Kovalenko

Gladkikh

2019

Vestn. VOGiS

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In Vitro Embryo Culture of Some Sweet Cherry Genotypes

Cited by 5 publications

References 5 publications

Exploring Genetic Diversity in Romanian Lycium Species: A Comparative Study of Wild and Cultivated Genotypes

Exploring Genetic Diversity in Romanian Lycium Species: A Comparative Study of Wild and Cultivated Genotypes

Effect of the timing of embryo isolation on the output of seedlings of sour cherry varieties when cultivated <i>in vitro</i>

In vitro cultivation of the embryos of hybrid forms of early-ripening sweet cherry (Prunus avium L.) varieties

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