In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)

Bermejo, Carolina; Gatti, Ileana; Cointry, Enrique

doi:10.1007/s11240-016-1065-7

Cited by 48 publications

(30 citation statements)

References 23 publications

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“…Through use of this technique combined with the SSD breeding method, and once more considering phase III as the end of each cycle, three generations of selfed pepper plants could be conducted per year. In lentils, four generations per year could be conducted using embryos excited 18 days after anthesis (Bermejo et al 2016), demonstrating the viability of this technique.…”

Section: Discussionmentioning

confidence: 95%

“…This study demonstrates the efficiency of the in vitro culture technique of immature zygotic embryos (IZE) in shortening the breeding cycle of ornamental peppers. The same technique used to reduce the breeding cycle of sunflower (Dağüstü et al 2012), wheat (Sharma and Gill 1982), orchid (Yeung et al 1981), tomato (Gebologlu et al 2011), pepper and green pepper (Manzur et al 2014), and lentil (Bermejo et al 2016) have already been described.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro cultivation of immature zygotic embryos (IZE) can reduce the breeding cycle, considering that it is not necessary to wait for seed maturation in the fruit (Gatti et al 2016). This biotechnological technique can be used in combination with conventional methods such as single seed descent (SSD), which allows homozygous lines and/or recombinant inbred lines (RIL) to be obtained in a relatively short time (Bermejo et al 2016). Manzur et al (2013) and Manzur et al (2014), working with five genotypes of pepper in the climate conditions of the Mediterranean coast of Spain, reduced the reproductive cycle from 148-184 days in the conventional method to 93-133 days, depending on the genotype, through embryo culture.…”

Section: Introductionmentioning

confidence: 99%

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How to shorten a plant breeding program? A case study with ornamental peppers

Barroso

Rêgo

Crispim

et al. 2019

Crop Breed. Appl. Biotechnol.

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Plant breeding of ornamental peppers (Capsicum spp.) can be supported by biotechnological tools. The objective of this study was to evaluate the efficiency of an in vitro culture of immature zygotic embryos (IZE) to reduce the breeding cycle of ornamental pepper (C. annuum) in comparison to the conventional system. Three ornamental pepper genotypes were used: UFPB 001, UFPB 004, and UFPB 099. Embryos at 30 days after selfing were inoculated in MS ½-strength culture medium, and at the same time, seeds were placed to germinate in a commercial substrate. Approximately 215 days are required from selfing until fruit ripening in the conventional system, whereas the IZE system requires an average of 153 days, a decrease of approximately 30% per selection cycle, corresponding to 496 days considering 8 selfing cycles. A decrease in time, labor, and inputs makes the IZE system a suitable tool for shortening the breeding program of ornamentals peppers.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

How to shorten a plant breeding program? A case study with ornamental peppers

Barroso

Rêgo

Crispim

et al. 2019

Crop Breed. Appl. Biotechnol.

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“…Other protocols use an in vitro step in which immature seeds are dissected out and cultured on artificial media such as for field pea (Ochatt et al., ), tomato (Bhattarai, de la Pena, Midmore, & Palchamy, ) or sunflower (Jambhulkar, ). Regarding lentil, an in vitro embryo culture method has been published allowing the development of four generations per year (Bermejo, Gatti, & Cointry, ). However, in vitro methods are expensive and time‐consuming due to the required dissection of small, fragile seeds, sterile growing conditions and special media required for growth and are thus of limited use in breeding programmes (Gatti et al., ).…”

Section: Introductionmentioning

confidence: 99%

Rapid generation cycling of an F₂ population derived from a cross between Lens culinaris Medik. and Lens ervoides (Brign.) Grande after aphanomyces root rot selection

Lulsdorf

Banniza

2018

Plant Breeding

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Cultivated lentil (Lens culinaris Medik.) is susceptible to aphanomyces root rot (ARR), whereas partial resistance is present in wild lentil including Lens ervoides (Brign.) Grande. Approximately six generations of selfing are required to fix a desired trait in a population, which usually requires 2 years in a breeding programme, so the primary objective was to develop a rapid generation cycling (RGC) technique that achieves this goal in 1 year. Rapid generation cycling was then tested on an F2 population (LR‐59) derived from a L. culinaris × L. ervoides cross in combination with a reliable ARR screening technique, which generates a wide range of disease severities conducive to selection. Phenotyping of an F2 population of more than 1,200 plants resulted in scores ranging from 2.4 to 4.0 on a scale from zero to five. Plants with scores lower than 4.0 were selected for advancement for five generations using a modified single‐seed descent method, optimum growing conditions, 20‐hr photoperiod and harvest of immature seeds. Seeds were germinated in a 100 μM gibberellin solution. Average generation length after phenotyping was 56 days resulting in five generations within approximately 300 days. Using a modified inoculation protocol, ARR phenotyping of the F7 population resulted in scores ranging from 1.4 to 4.0. This inexpensive, nonsterile speed breeding protocol saves 1 year in the development of lentil varieties with improved ARR resistance.

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“…The appropriate method and how early seed can be made viable are determined by the species/genotype and will impact how many additional generations can be achieved. Bermejo et al (2016) used PGR in embryo culture media to promote germination of immature lentil (Lens culinaris) seed to achieve 4 generations annually. Mobini et al (2015) sprayed both lentil and faba bean (Vicia faba) plants with PGR to promote early flowering and carried out embryo rescue with PGR-enriched agar media to achieve up to 8 and 6.8 generations per year,…”

Section: Embryo Rescue Of Immature Seedmentioning

confidence: 99%

Integrating genomic selection and speed breeding to increase genetic gain in spring wheat (Triticum aestivum) breeding

Watson¹

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There is a need to develop novel breeding approaches to increase the rate of genetic gain for wheat yield due to rising demand and climate volatility. Genomic selection (GS) can increase genetic gain in quantitative traits, such as yield, principally through a shorter selection cycle. 'Speed breeding' (SB) could further accelerate the breeding cycle by using extended photoperiod in the glasshouse, enabling rapid generation advance. An integrated breeding approach could include fast inbred line development, phenotyping of yield secondary traits for multivariate GS and indirect phenotypic selection for important traits prior to field trials in a target environment. The objective of this thesis was to explore a comprehensive breeding strategy that combines the aforementioned tools, aimed at increasing the rate of genetic gain in wheat. Firstly, to investigate the potential for SB application across major crop species, a sample of wheat, barley, canola and chickpea cultivars were evaluated under either 22-hour light (SB) or natural, diurnal lighting in a temperature-controlled glasshouse to quantify and compare the effects on development. Wheat and barley were also grown at high densities to investigate potential single seed descent (SSD) programs. Time to anthesis was significantly reduced for all species relative to the natural photoperiod conditions. Coupled with harvesting seed two weeks post-anthesis, SB could enable up to six generations of wheat per year. Grain and spike number were similar between lighting treatments and germination percentage was higher in grain of SB-grown plants at two weeks after flowering. These findings indicated that SB had potential to be used at multiple stages of a breeding program. In order to identify traits that could be measured under SB (SB traits) that were correlated to field-based yield and could be used in multivariate GS, an explorative study was carried out on a 135-line, bi-parental (SeriM82 x Hartog, SxH) bread wheat population. Days to anthesis (SB-DTA), plant height (SB-height), spike length and flag leaf length were measured under SB and narrow-sense heritability and genetic correlation with field-based yield was determined from previously performed field trials in three environments that varied with respect to extent and timing of water deficit. Field-based traits were also measured, including DTA, plot height and two senescence indices. The heritability of most SB and field-based traits were higher than yield heritability and SB-DTA and SB-height showed strong genetic correlations with yield (up to-0.80 and-0.66 respectively) in the irrigated environment. Further investigation with a larger, more diverse population was carried out using a previously developed 256-line, multi-reference bread wheat population, and data from two previous rain-fed yield trials. Multivariate models iii incorporating the field-based senescence indices significantly increased yield prediction ability although the addition of SB traits did not improve prediction above that of univariate mo...

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In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)

Cited by 48 publications

References 23 publications

How to shorten a plant breeding program? A case study with ornamental peppers

How to shorten a plant breeding program? A case study with ornamental peppers

Rapid generation cycling of an F₂ population derived from a cross between Lens culinaris Medik. and Lens ervoides (Brign.) Grande after aphanomyces root rot selection

Integrating genomic selection and speed breeding to increase genetic gain in spring wheat (Triticum aestivum) breeding

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In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik)

Cited by 48 publications

References 23 publications

How to shorten a plant breeding program? A case study with ornamental peppers

How to shorten a plant breeding program? A case study with ornamental peppers

Rapid generation cycling of an F2 population derived from a cross between Lens culinaris Medik. and Lens ervoides (Brign.) Grande after aphanomyces root rot selection

Integrating genomic selection and speed breeding to increase genetic gain in spring wheat (Triticum aestivum) breeding

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Rapid generation cycling of an F₂ population derived from a cross between Lens culinaris Medik. and Lens ervoides (Brign.) Grande after aphanomyces root rot selection