In vitro Enhancement of Pancreatic β Cells MIN6 Proliferation by Insulinotropic Gymnema sylvestre Aqueous Extracts: Evidence-based Regenerative Therapeutic Capacity of a Medicinal Herb

Kasabri, Violet; Afifi, Fatma U.; Bustanji, Yasser; Mashallah, Sundus; Naffa, Randa; Mehdi, Hala

doi:10.9734/bjmmr/2015/12681

Cited by 4 publications

(5 citation statements)

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“…In vitro glucose movement was assayed according to Kasabri et al (2015). To imitate the viscosity-based diffusion hindrance of gel-forming dietary fibres, and hence, their postprandial glucose lowering efficacies in vitro, guar gum 50 mg/mL was used as a classical positive control, and 10, 25 and 50 mg/mL of A. capillus-veneris AEs in 0.22 M glucoses in triplicates were dialysed against 0.15 M NaCl overnight at 37 C with gentle shaking and a parallel plant-free (negative) control was included (Gallagher et al 2003;Butt et al 2007).…”

Section: Glucose Movement In Vitro Assaymentioning

confidence: 99%

“…With the treatment plant administered in doses 125, 250 and 500 mg/kg b.wt; OSTT and OGTT were conducted according to Kasabri et al (2015) …”

Section: Oral Starch Tolerance Test (Ostt) and Oral Glucose Tolerancementioning

confidence: 99%

“…This was further ascertained by the lack of in vitro efficacies of A. capillus-veneris AEs on the impedance of glucose movement in vitro. Using the diffusion model described in Kasabri et al (2015), viscous water- Table 2. Effects of ascending concentrations of different parts of A. capillus-veneris AE (mg/mL) and its bioactive phytoconstituent chlorogenic acid (lg/mL) on % reduction of enzymatic starch digestion in vitro.…”

Section: Osttmentioning

confidence: 99%

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Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts: in vitro and in vivo evaluations

Kasabri

Al-Hallaq²,

Bustanji

et al. 2016

Pharmaceutical Biology

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Context: Adiantum capillus-veneris L. (Adiantaceae) hypocholesterolemic activity is therapeutically praised. Objectives: Pharmacological modulation of pancreatic triacylglycerol lipase (PL) and a-amylase/a-glucosidase by A. capillus-veneris are evaluated. Materials and methods: Using positive controls (acarbose, orlistat, guar gum, atorvastatin, glipizide and metformin) as appropriate, crude aqueous extracts (AEs) of A. capillus-veneris aerial parts were tested via a combination of in vitro enzymatic (0.24-100 mg/mL), acute in vivo carbohydrate tolerance tests (125, 250 or 500 mg/kg body weight [b.wt]) and chronic in vivo studies (500 mg/kg b.wt) in high cholesterol diet (HCD) fed Wistar rats. Results: Like acarbose, A. capillus-veneris as well as chlorogenic acid, with respective IC 50 values (mg/mL) of 0.8 ± 0.0 and 0.2 ± 0.0, were identified as in vitro potent dual inhibitors of a-amylase/a-glucosidase. Unlike guar gum, A. capillus-veneris had no glucose diffusion hindrance capacity. Equivalent to orlistat, A. capillus-veneris and its phytoconstituents inhibited PL in vitro with an ascending order of PL-IC 50 values (lg/mL): ferulic acid; 0.48 ± 0.06 < ellagic acid; 13.53 ± 1.83 < chlorogenic acid; 38.4 ± 2.8 < A. capillus-veneris; 1600 ± 100. Incomparable to acarbose or metformin and glipizide, A. capillus-veneris (125, 250 and 500 mg/kg b.wt) lacked antihyperglycaemic efficacies in acute starch-or glucose-evoked postprandial hyperglycaemia increments in normoglycaemic overnight fasting rats. Superior to atorvastatin; A. capillusveneris exerted significant antiobesity (p < 0.001) with marked triacylglycerol-reducing capacities (p < 0.001) in comparison to rats fed with HCD for 10 weeks. Discussion and conclusion: A. capillus-veneris, modulating pancreatic digestive enzymes, may be advocated as a combinatorial diabesity prevention/phytotherapy agent. ARTICLE HISTORY

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Section: Glucose Movement In Vitro Assaymentioning

confidence: 99%

“…With the treatment plant administered in doses 125, 250 and 500 mg/kg b.wt; OSTT and OGTT were conducted according to Kasabri et al (2015) …”

Section: Oral Starch Tolerance Test (Ostt) and Oral Glucose Tolerancementioning

confidence: 99%

Section: Osttmentioning

confidence: 99%

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Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts: in vitro and in vivo evaluations

Kasabri

Al-Hallaq²,

Bustanji

et al. 2016

Pharmaceutical Biology

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“…The complimentary effects on HbA1c and fasting blood sugar in patients with uncontrolled Type 2 diabetes mellitus after 12 weeks showed by GCT [66]. The study revealed that G. sylvestre leaves aqueous extracts augmented β-cell expansion and potentiated glucose-evoked Ca 2+ -regulated insulin secretion [67].…”

Section: Antidiabetic Activitymentioning

confidence: 99%

A Review on Phytochemistry and Pharmacological Properties of Milkweed Family Herbs (Asclepiadaceae)

Karale¹,

Karale²

2017

Asian J Pharm Clin Res

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Calotropis procera and Gymnema sylvestre are a perennial shrub and woody climber, respectively, and belonging to the family Asclepiadaceae. They are commonly known as milkweed family herbs. The Calotropis commonly have two species, i.e., C. procera and C. gigantean. All parts of plant exude white milky latex when cut. It has been widely used in traditional medicine due to its pharmacological active compounds found in all parts of plants such as bark, roots, and leaves and especially its latex which exudes from damaged or broken stem and leaves. G. sylvestre an ayurvedic herb came to be known as "destroyer of sugar" because in ancient times Ayurveda physicians observed that chewing a few leaves of G. sylvestre suppressed the taste of sugar. It also reported to possess antidiabetic, antioxidant, and immunomodulatory, antiulcer, and anticancer potential. The gymnemic acid is the main constituent which is responsible for number of pharmacological activities. The present review assembles the data on pharmacognostic and pharmacological potential of C. procera and G. sylvestre.

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“…This study was designed to evaluate and evidence the role of both Arum species in the treatment of diabetes and obesity since the high prevalence of dyslipidemia, obesity and diabetes in Jordan has reached alarming dimensions. Pancreatic triacylglycerol lipase (PL), α-amylase and α-glucosidase are interesting pharmacological targets for the management of diabetes-obesity (diabesity) (Kasabri et al , 2015. On the other hand, the determinant role of plants in the ethnomedical integrative/complementary treatment of many diseases including obesity-diabetes in Jordan is well reported (Bustanji et al 2010;Afifi-Yazar et al 2011;Al-Hallaq et al 2012;Afifi & Kasabri 2013).…”

Section: Introductionmentioning

confidence: 99%

In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth & Sm. andArum palaestinumBoiss.

Afifi

Kasabri

Liţescu

et al. 2015

Natural Product Research

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Arum dioscoridis and A. palaestinum (Araceae) are indigenous plant species in Jordan. HPLC-MS analysis of A. dioscoridis revealed the presence of apigenin, luteolin, quercetin, quercetin-3-O-β-glucoside, vitexin, isoorientin, esculin, and caffeic and ferulic acids. Both Arum spp., influenced gastrointestinal carbohydrate and lipid digestion and absorption. Orlistat inhibited dose dependently and highly substantially pancreatic lipase (PL) in vitro. Similar to orlistat, Arum species aqueous extracts (AEs), apigenin, caffeic acid and esculin exhibited a concentration related PL inhibition. Comparable to acarbose, dual inhibition of α-amylase/α-glucosidase was observed for both Arum species. Like guar gum, A. dioscoridis AE minimised substantially area under 24 h glucose curve. Acute starch-induced postprandial hyperglycaemia in overnight fasting rats was highly significantly (p < 0.001) decreased by A. dioscoridis AE. A. palaestinum could not perform effectively in either starch- or glucose-fed fasting rats. No antiproliferative effects against colorectal cancer cell lines HT29, HCT116 and SW620 were detected for tested Arum spp.

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In vitro Enhancement of Pancreatic β Cells MIN6 Proliferation by Insulinotropic Gymnema sylvestre Aqueous Extracts: Evidence-based Regenerative Therapeutic Capacity of a Medicinal Herb

Cited by 4 publications

References 0 publications

Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts: in vitro and in vivo evaluations

Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts: in vitro and in vivo evaluations

A Review on Phytochemistry and Pharmacological Properties of Milkweed Family Herbs (Asclepiadaceae)

In vitroandin vivocomparison of the biological activities of two traditionally and widely usedArumspecies from Jordan:Arum dioscoridisSibth & Sm. andArum palaestinumBoiss.

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