In vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari: Psoroptidae)

Lekimme, Mireille; Mignon, Bernard; Tombeux, S.; Focant, Charles; Maréchal, Françoise; Losson, Bertrand

doi:10.1016/j.vetpar.2006.02.041

Cited by 26 publications

(20 citation statements)

References 33 publications

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“…Infestation was identified by macroscopic lesions and confirmed through microscopic examination, selecting and counting P. cuniculi with a mobility and morphology coincident with those described in earlier studies (Lekimme et al 2006, Bowman 2009). …”

Section: Methodssupporting

confidence: 53%

A trial of doramectin injection and ivermectin spot-on for treatment of rabbits artificially infested with the ear mite “Psoroptes cuniculi”

Elhawary

Sorour

El-Abasy

et al. 2017

Polish Journal of Veterinary Sciences

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The ear mite "Psoroptes cuniculi" is the main cause of ear mange, a highly contagious parasitic skin disease in rabbits all over the world. In the current work, a preliminary therapeutic trial to study the effect of the broad use acaricides doramectin and ivermectin on P. cuniculi was performed on artificially infested rabbits.Twenty five adult New Zealand white rabbits were used in this study. The rabbits were assigned randomly into five groups/ 5 rabbits in each group. Each rabbit was experimentally infested with 100 mites/ ear. The first group was designated the positive control group and was not treated. The second and third groups were treated with doramectin 200 and 400 μg/kg bw, respectively. Groups 4 and 5 were treated by dressing with ivermectin in one dose and 2 doses with a 1 week interval. After the therapy, all rabbits were examined microscopically on the 7 th , 14 th , and 28 th day post treatment and the number of live mites (larvae, nymphs, and adults) on each rabbit was counted at the end of the experiment (28 th day).The results showed that the rabbits treated subcutaneously with doramectin at a single dose of 200 μg /kg bw showed a very low effect, although there was significant improvement when the dose was doubled to 400 μg /kg bw, with the number of mites counted decreasing significantly. Rabbits treated topically with ivermectin spot-on, a single dose or 2 doses, showed great improvement of the lesion: the number of mites was reduced to zero. In conclusion, this work showed that ivermectin spot-on applied locally on infested ears proves to be more effective against P. cuniculi than doramectin injected subcutaneously. Further trials on ear mange therapeutics in rabbits are to be encouraged.

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Section: Methodssupporting

confidence: 53%

A trial of doramectin injection and ivermectin spot-on for treatment of rabbits artificially infested with the ear mite “Psoroptes cuniculi”

Elhawary

Sorour

El-Abasy

et al. 2017

Polish Journal of Veterinary Sciences

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“…Until now, only two species of entomopathogenic fungi have shown lethal activity against this mite, i.e. M. anisopliae (Smith et al 2000) and B. bassiana (Lekimme et al 2006a). Two parameters are important for the control of psoroptic mange with fungi.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, control of psoroptic scab is based on the use of chemical acaricides. Nevertheless treatment failures and environmental considerations have led to the development of alternative approaches, particularly the use of entomopathogenic fungi (Smith et al 2000;Wall 2001, 2005;Brooks et al 2004;Lekimme et al 2006a;Abolins et al 2007). Because infection by entomopathogenic fungi such as Beauveria and Metarhizium species results from direct penetration of the tegument without any requirement for ingestion, these organisms often display wide host ranges.…”

Section: Introductionmentioning

confidence: 99%

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Pathogenicity and thermotolerance of entomopathogenic fungi for the control of the scab mite, Psoroptes ovis

et al. 2008

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Psoroptes ovis is responsible for a highly contagious skin condition, both in sheep and cattle. This parasite has a marked economical impact in the sheep and cattle industry. Biological control is considered as a realistic alternative to chemotherapeutic control. Laboratory experiments were carried out to evaluate the pathogenicity and the thermotolerance of twelve isolates of entomopathogenic fungi from four genera (Beauveria Vuillemin, Metarhizium Sorokin, Paecilomyces Bainier and Verticillium Nees). The pathogenicity was evaluated by the survival of P. ovis females after exposure to 10(6) to 10(8) conidia ml(-1) in humidity chambers. Results revealed intra- and interspecies differences. All isolates with the exception of B. bassiana IHEM3558 and V. lecanii MUCL8672 induced 50% mortality within 2 days at the highest concentration. At this concentration the entire mite population became infected with all isolates but B. bassiana IHEM3558; however, only four isolates gave rise to 100% infected cadavers at the lowest concentration. The thermotolerance of each isolate was evaluated by measuring its growth on an artificial medium kept between 25 and 37.5 degrees C. All isolates were able to grow up to 30 degrees C but only two, M. anisopliae IHEM18027 and Paecilomyces farinosus MUCL18885, tolerated temperatures up to 35 degrees C. These two isolates could be considered as good candidates for further use as biopesticide taking into account their virulence and thermotolerance. Other critical factors linked with the implementation of this type of biocontrol in P. ovis infected animals are discussed.

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“…Previous studies carried out with the aim to find a biological control method for psoroptic mange showed the pathogenicity of other fungal species, such as Metarhizium anisopoliae and Beauveria bassiana for Psoroptes mites in vitro and in vivo (Smith et al 2000;Brooks and Wall 2001;Brooks et al 2004;Lekimme et al 2006;Abolins et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Isolation of Scopulariopsis spp. fungi from Psoroptes cuniculi body surface and evaluation of their entomopathogenic role

et al. 2008

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In this study, some in vitro trials were carried out to evaluate the association between Scopulariopsis spp. fungi with Psoroptes cuniculi (Acari: Psoroptidae) and their potential pathogenicity to this mite species. After cultivation on Sabouraud dextrose agar with chloroamphenicol at 26 degrees C for 20 days and macro- and microscopical examinations, from P. cuniculi mites taken from some infested rabbits fungi belonging to the genus Scopulariopsis were isolated. Investigations were carried out to evaluate in vitro the potential pathogenic role of Scopulariopsis to P. cuniculi; to this aim, Scopulariopsis brevicaulis isolated from a cat was tested. In several culture media, the dose-dependent P. cuniculi mortality with different concentrations of S. brevicaulis and the ability of S. brevicaulis to penetrate inside the body of infected mites were evaluated. Results obtained demonstrated that, in the rabbit, Scopulariopsis fungi can be associated with P. cuniculi, and that S. brevicaulis can be an entomopathogen for P. cuniculi in a dose-dependent manner. A more rapid mortality of the mites and a quicker S. brevicaulis growth in plates containing rabbit serum with respect to all other media used were also found. S. brevicaulis demonstrated the ability to invade the body of P. cuniculi.

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In vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari: Psoroptidae)

Cited by 26 publications

References 33 publications

A trial of doramectin injection and ivermectin spot-on for treatment of rabbits artificially infested with the ear mite “Psoroptes cuniculi”

A trial of doramectin injection and ivermectin spot-on for treatment of rabbits artificially infested with the ear mite “Psoroptes cuniculi”

Pathogenicity and thermotolerance of entomopathogenic fungi for the control of the scab mite, Psoroptes ovis

Isolation of Scopulariopsis spp. fungi from Psoroptes cuniculi body surface and evaluation of their entomopathogenic role

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