In vitro evaluation of antibacterial nanomaterial-induced anaphylactoid reaction for indwelling catheters

Abstract

Background To prevent tunnel infection of indwelling catheters, impregnation with antiseptics or antibiotics is effective. However, 13 patients using chlorhexidine–silver sulfadiazine-impregnated catheters experienced serious anaphylactic shock in Japan. Thus, it is necessary to select a suitable evaluation method for allergic reactions and develop a novel antibacterial coating material that does not cause anaphylactic reaction. Methods Two types of highly dispersible and antibacterial nanoparticles—fluorine (F)-doped hydroxyapatite (HAp) and zinc (Zn)-doped HAp—were tested using of the system and compared with compound 48/80 (c48/80) as a histamine releaser and chlorhexidine gluconate (CHG) as an anaphylactic inducer. Results The histamine concentrations secreted from HMC-1 cells remained mostly the same even with the addition of F-HAp and Zn-HAp. On the contrary, the levels of the chemical mediators from the cells by the addition of F-HAp and Zn-HAp were significantly lower than those of only c48/80 and CHG without the addition of HAp. Conclusions The assay was a well-evaluated method for quantifying histamine concentrations released from HMC-1 cells. Our study induced HMC-1 cells accompanied with and without the nanomaterials; the potential of F-HAp and Zn-HAp to induce allergic reactions was found to be quite low. Therefore, the antibacterial nanomaterials are expected to hardly induce anaphylactoid reactions.

Continuous antimicrobial mechanism of dispersible hydroxyapatite nanoparticles doped with zinc ions for percutaneous device coatings

“…49 In our earlier research, Zn-HAp nanomaterials with 9% [Zn/(Zn + Ca) × 100] 15 were applied in the anaphylactoid reaction assays using HMC-1 cells. 26 In this study, the Zn ion content in Zn-HAp was increased to 13.5% (the Zn(15)-HAp) which was almost the maximum value of Zn ion-substitution in HAp crystalline lattice formed without ZnO contamination during the synthesis process. 15 Based on this result, it was thought that Zn(15)-HAp nanoparticles are poor at inducing an allergic-like reactions in vitro.

Figure 5.Histamine amounts released from HMC-1 cells.

…”

“…Prior to the clinical estimation of anaphylaxis incidence in the implantation of antisepsis-impregnated catheters, 10 in vitro pre-assays of anaphylactoid reactions to antibacterial materials are valuable. Histamine release detection tests involving HMC-1 cells as anaphylactoid reaction assays 26 were conducted by the addition of Zn-HAp nanoparticles (Figure 5). The amount of histamine released from HMC-1 cells after addition of Zn(15)-HAp nanoparticles was not statistically different to that of the negative control (cells alone) and nHAp, but very much lower than that of CHG.…”

“…Histamine release assay from human mast cell-1 (HMC-1) cells was conducted using an in vitro screening method involving anaphylactoid reactions, modifying the method of Hamano et al, 25 against antibiotic nanoparticles as described in our literature. 26 Briefly, HMC-1 cells (at a density of 1.0 × 10 4 in 150 μL) were seeded in 96-well plates. After 6 h of incubation, the plate was centrifuged at 150 × g for 5 min and histamine concentrations in the supernatant were measured using a histamine ELISA Kit (Bertin Pharma, MI, USA).…”

Reduced Sliding Friction of Lubricant-Impregnated Catheters

“…Indwelling catheters are left in place after insertion, and urine is drained into a collection bag; a water-filled balloon holds the catheter in place within the bladder . This latter type is also known as the Foley catheter, which was invented in 1929 by Frederic Foley. , For both types of catheters, several studies have compared the performance of uncoated versus coated catheters. − The most common choice is a hydrophilic polymer coating that becomes slippery when wet. Clinical studies have shown that the use of hydrophilic catheters significantly reduces the incidence of CAUTIs.…”

“… 1 , 23 For both types of catheters, several studies have compared the performance of uncoated versus coated catheters. 24 − 29 The most common choice is a hydrophilic polymer coating that becomes slippery when wet. Clinical studies have shown that the use of hydrophilic catheters significantly reduces the incidence of CAUTIs.…”