In Vitro Evaluation of Antibacterial and Antifungal Activity of Biogenic Silver and Copper Nanoparticles: The First Report of Applying Biogenic Nanoparticles against Pilidium concavum and Pestalotia sp. Fungi

Abstract

There is increased attention paid to metallic nanoparticles due to their intensive use in various branches of agriculture and biotechnology, such as pest management, nanosensors, gene delivery, seed treatment, etc. There has been growing interest in applying environmentally friendly strategies for synthesizing nanoparticles without using substances which are hazardous to the environment. Biological practices for the synthesis of nanoparticles have been considered as possible ecofriendly alternatives to chemical synthesis. In the present study, we used biogenic silver and copper nanoparticles which were prepared by a previously reported green method. Moreover, the problem of chemical residues, which usually remain along with chemically synthesized nanoparticles and limit their application, was solved by developing such a green synthesis approach. To study the antibacterial activity of silver and copper nanoparticles, Pseudomonas aeruginosa was used; for the evaluation of antifungal activity, the pathogenic fungi Botrytis cinerea, Pilidium concavum and Pestalotia sp. were applied. To the best of our knowledge, this study represents the first time that the antifungal impact of a nanoparticle has been tested on Pilidium concavum and Pestalotia sp. Silver nanoparticles were found to be the more effective antimicrobial agent against all examined pathogens in comparison to copper nanoparticles. Data from such investigations provide valuable preliminary data on silver nanoparticle-based compounds or composites for use in the management of different pathogens.

Inhibition of Phytopathogenic and Beneficial Fungi Applying Silver Nanoparticles In Vitro

“…Furthermore, other authors have reported that fungal hyphae showed the formation of pits and pores against different pathogenic isolates of the same A. solani fungus after being treated with silver nanoparticles [ 20 ]. In addition, Bayat et al [ 40 ] reported that AgNP could successfully inhibit spore germination in B. cinerea in a concentration-dependent manner. AgNP application on Phytophthora parasitica and P. capsici caused a significat inhibition of the on-zoospore germination and germ tube elongation [ 26 ].…”

Biomedical applications of cerium vanadate nanoparticles: a review

“…16 In vitro experiments were performed to evaluate the antimicrobial susceptibility of microorganisms and the antimicrobial activity of biosynthesized NPs on Pseudomonas aeruginosa bacteria. 98 The results showed that CeVO 4 NPs have excellent antibacterial activity against Streptococcus mutans and Streptococcus pyogenes with MIC values at 200 mg mL À1 and against Vibrio cholera, Salmonella typhi, and Shigella flexneri with MIC values at 350 mg mL À1 . 16 Microsporum canis, Trichophyton rubrum, Trichophyton mentagrophytes, and Aspergillus niger were all eliminated by CeVO 4 NPs, and the NPs showed antifungal action against Candida albicans, Aspergillus niger and Aspergillus clavatus.…”

Influence of Inorganic Metal (Ag, Cu) Nanoparticles on Biological Activity and Biochemical Properties of Brassica napus Rhizosphere Soil

“…ZnO NPs at concentrations above 3 mmol L −1 (244.2 mg L −1 ) significantly inhibit growth of pathogenic postharvest fungi Botrytis cinerea and Penicillium expansum [23,24]. AgNPs tested at concentration 1-100 ppm were found to be the more effective antimicrobial agent against fungal pathogens Botrytis cinerea and Pilidium concavum in comparison to CuNPs tested at same concentrations [25]. Botrytis cinerea growth inhibition was not demonstrated in an in vitro well test with CuO NPs at concentrations 5, 50, 100, and 200 mg L −1 [26].…”