In vitro Evaluation of Antioxidant Activity of Brugeiera Gymnorrhiza and Aegialitis Rotundifolia

Ark, Reddy; Grace, .

doi:10.4172/2167-0412.1000231

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Further, the leaf of the plant is pounded with oil to make a paste which acts as an antidote for insect bites [10]. According to the present literature available for this plant, there have been very few scientific reports of pharmacological screening conducted such as in-vitro antioxidant [11], antimicrobial [12,13], antibacterial [14], anticancer [15], analgesic, antipyretic [10], anti-inflammatory [10,14], and in-vitro thrombolytic activities [14]. Recently, we have reported the presence of gallic acid, chlorogenic acid, caffeic acid, p-coumaric acid, rutin, coumarin, and quercetin by performing quantitative High-performance liquid chromatography (HPLC) analysis and an organosilicon compound, (−)-spiro[1-[(tert-Butyldimethylsiloxy)methyl]-3,5,8-trimethyl-bicyclo [4.3.0]non-2-en-5,7-diol-4,1′-cyclopropane] was detected in GC-MS analysis as the most abundantly found compound [7].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

2019

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Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote Abstract Background: Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of its folkloric usage as a potent pain reliever. Therefore, the present study deals in acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia ethanolic leaves extract (ARELE) on male and female Wistar rats administered through oral gavage.Methods: Acute toxicity study was conducted at a single oral dose of 1000, 1500, and 2000 mg/kg, body weight (b.wt.) for 14 days with a special emphasis on the first four hours after drug administration to find out any mortality and morbidity. In sub-acute toxicity, the extract at the doses of 125, 250 and 500 mg/kg, b.wt., was administered orally for 30 days. Important parameters such as general behaviour, body and organ weight, urinalysis, haematological and biochemical profile, organ macroscopy and microscopy were conducted. Organ sample of liver, kidney, pancreas, heart, lungs, and stomach were taken from both male and female rats, whereas the sample of testis and ovary was taken from male and female rats respectively for organ necropsy and histopathological studies. Neurobehavioral toxicity was conducted by performing a functional observational battery (FOB) and locomotor activity on the initial and final week of the 30-day study period. Results:No mortality or any major signs of morbidity was recorded for acute toxicity except for the limit dose (2000 mg/kg, b.wt.) which produced a slight short-term sedative effect. In sub-acute toxicity, no major alteration was observed in the evaluated parameters. However, few minor changes were recorded for high dose (500 mg/kg, b.wt.) group. Conclusion:The results of the present study showed that oral administration of Aegialitis rotundifolia ethanolic leaves extract did not produce any severe toxic effects in both acute and sub-acute studies in Wistar rats. Therefore, usage of an appropriate amount of Aegialitis rotundifolia leaves extract preferably at low doses for its traditional use should be considered safe.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

2019

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Phytochemical Profiling Using Lc-Q-Tof-MS Analysis and in Vitro Antioxidant Activity of a Rare Salt-Secreting Mangrove Aegialitis Rotundifolia Roxb. Leaves Extract

Ghosh

Mondal

Ramakrishna

2019

Int J Pharm Pharm Sci

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Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

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Physalis angulata L. (Solanaceae): Antimicrobial and Antioxidant Studies of the Aqueous Methanol Leaves Extract

E.O.,

C.A.,

F.A.

et al. 2024

NJPAS

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Physalis angulata has several ethnomedical uses. The study was aimed at investigating the potential antimicrobial and antioxidant of the aqueous-methanol extract of P.angulata leaves. The extract was cold macerated in 80% methanol and tested for phytochemicals, antioxidants, and antimicrobials. The agar well diffusion method was used to determine antimicrobial activities at 6.25–100 mg/mL. The microorganisms tested included Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Candida albicans, and Aspergillus niger. The quantification of total phenols and flavonoids was conducted using the Folin-Ciocalteu reagent method and the Aluminium Chloride colorimetric method, respectively. The assessment of radical scavenging activity was performed using the DPPH and FRAP models. The plant extract exhibited antibacterial activity, demonstrating concentration-dependent activity against all tested organisms. At 25 mg/mL, S. aureus and A. niger recorded zones of inhibitions of 19.00 and 23.00 mm while B. subtilis, E. coli, P. vulgaris and C. albicans recorded 18.83, 18.67, 14.00 and 4.35 respectively. However, at the maximum concentration of 100 mg/mL, A. niger recorded the highest zone of inhibition of 26.33 mm. The MIC was observed at 12.5 mg/mL against S. aureus, A. niger as well as 25 mg/ml B. substilis, E.coli, P. vulgaris and C. albicans respectively. The total flavonoids and phenolic activities was reported to be of 56.11±0.03 mg QAE/100 g and 96.13±0.07 mg QAE/100 g respectively. The DPPH and FRAP scavenging activities recorded IC50 of 0.05 and 0.07 mg/mL against 0.01 and 0.02 mg/mL of the control. The methanol extract of P. angulata leaves exhibited promising antioxidant and antimicrobial properties, supporting its traditional usage in ethno-medicine for treating many diseases.

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In vitro Evaluation of Antioxidant Activity of Brugeiera Gymnorrhiza and Aegialitis Rotundifolia

Cited by 3 publications

References 11 publications

RETRACTED ARTICLE: Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

RETRACTED ARTICLE: Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

Phytochemical Profiling Using Lc-Q-Tof-MS Analysis and in Vitro Antioxidant Activity of a Rare Salt-Secreting Mangrove Aegialitis Rotundifolia Roxb. Leaves Extract

Physalis angulata L. (Solanaceae): Antimicrobial and Antioxidant Studies of the Aqueous Methanol Leaves Extract

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