Phytochemical Profiling Using Lc-Q-Tof-MS Analysis and in Vitro Antioxidant Activity of a Rare Salt-Secreting Mangrove Aegialitis Rotundifolia Roxb. Leaves Extract

Ghosh, Debjit; Mondal, Sambhu Charan; Ramakrishna, K.

doi:10.22159/ijpps.2019v11i3.29985

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…FRAP estimates the ability of compounds to act as an electron donor [34]. DPPH assay based on DPPH solution decolorization after the addition of a radical or an antioxidant species is a simple, rapid, sensitive, and reproducible method for the evaluation of the free radical scavenging effect of plant extracts [35]. DPPH at room temperature is a stable free radical and becomes a stable diamagnetic molecule by accepting an electron or by hydrogen transfer [36,37].…”

Section: Discussionmentioning

confidence: 99%

Seasonal Variations in Antioxidant Capacities and Phenolic Contents of Tea Leaf Extracts

JAMIR

Ajungla

2020

Asian J Pharm Clin Res

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Objectives: The objective of the study was to estimate the seasonal variations in the antioxidant capacities, total polyphenol content (TPC), total flavonoid content (TFC), and tannin content (TC) of tea leaf extracts from two different plantation sites. Methods: Samples were collected from two tea gardens in Tuli and Ungma situated at N 26°39’19.3 E 094°39’22.7 and N 26°17’30.6 E 094°28’29.2, respectively, under the Mokokchung district of Nagaland, India. TPC, TFC, and TC from sample extracts were determined using Folin–Ciocalteu reagent, aluminum chloride colorimetric, and Folin–Ciocalteu assay. Apart from these, antioxidant capacities were analyzed using ferric reducing ability of plasma (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The concentrations of total polyphenol, flavonoid, and tannin varied from 552.029±8.079 to 305.647±1.744 mg gallic acid equivalent/g, 238.770±0.508–148.457±1.653 mg catechin equivalent/g, and 26.453±0.485–20.173±0.173 mg tannic acid equivalent/g, respectively. FRAP and DPPH assay displayed value ranging from 2.564±0.023 to 1.074±0.023 mmol Fe(II) equivalent/g and 3.612±0.053–2.076±0.028 mmol Trolox equivalent/g. Significant seasonal variations in concentrations of these compounds were observed and a positive correlation between antioxidant capacities and phenolics of tea leaf extracts was established. Conclusion: Tea (Camellia sinensis (L.) O. Kuntze) has been regarded as a plant of immense medicinal and therapeutic value since time immemorial. The tea leaf extracts analyzed in this study gave high TPC, TFC, and TC, as well as high antioxidant activity in terms of DPPH and FRAP value. Studying such properties in tea leaves contributes more to our understandings of health benefit potentials in tea leaves and the quality of tea leaves on the basis of seasons and sites where they are planted.

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Section: Discussionmentioning

confidence: 99%

Seasonal Variations in Antioxidant Capacities and Phenolic Contents of Tea Leaf Extracts

JAMIR

Ajungla

2020

Asian J Pharm Clin Res

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“…Such studies will be useful in herbal medicine and will reduce the burden on endangered medicinal plants. Ghosh et al, (2018) [26] evaluated the phytochemical constituents of Aegialitis rotundifolia leaves using LC-Q-TOF-MS analysis and detected 25 phytoconstituents mostly belonging to flavonoids and alkaloids. For characterization, quality assurance and evaluation of O. indicum samples, LC coupled with electrospray ionization-qTOF-mass spectrometry (ESI-qTOF-MS) was performed for identification of chemical constituents of O. indicum (Peng et al, 2019) [27] .…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of bioactive compounds from different medicinal plants: An overview

Pande¹,

Chanda²

2020

Int. J. Chem. Stud.

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Metabolomics has become an important tool in many research areas like drug discovery, diseases and nutrition, plant physiology, food quality and authenticity assessment, biomarker discovery, environmental and biological-stress studies, functional genomics, integrative systems biology, etc. It can be targeted or non-targeted. Metabolomics has been used as a vast tool for the investigation and quality measurement and assessment of natural products derived from plant and its parts. However, metabolite profiling in crude extracts is not an easy work as natural materials show a very diverse form of structure. It makes use of various analytical techniques like LC-QTOF-MS, GC-QTOF-MS, UPLC-QTOFMS, LC-ESI-QTOF/MS. In the present review, an attempt has been done to enlist metabolic profiling of 50 medicinal plants, along with their part, solvent used and number of bioactive compounds identified. Its various uses have also been discussed.

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Screening of Aegialitis Rotundifolia Roxb. Leaves for Their Antioxidant Potential, Polyphenol Content and Flavonoid Content

N.¹,

Pavani²,

S.³

et al. 2023

IND. DRU.

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The present research is focused on investigating the phytochemical analysis and antioxidant activity of Aegialitis rotundifolia Roxb. leaf. Different solvent extracts of A. rotundifolia Roxb. leaves are screened for secondary metabolites by qualitative and quantitative analysis. The resultant phytochemical screening proves that the plant leaves consist of various secondary metabolites. The quantitative analysis proves that phenolic compounds and flavonoids are dominant among them. Four different antioxidant activity methods, namely, ABTS radical scavenging activity, phosphomolybdate assay, peroxide activity and FRAP (Ferric Reducing Antioxidant Power), are used for assessing the activity of the leaf extract. Ethanolic and aqueous extracts of the leaf have high potency of antioxidant activity and reported dose-dependent activity. Similar promising antioxidant results are also reported for ABTS radical scavenging activity (82.4% reduction at 200 µg mL-1 of ethanol and 90.0% reduction at 200 µg mL-1 of aqueous extract), phosphomolybdate assay (82.4% reduction at 200 µg mL-1 of ethanol and 74.5 % reduction at 200 µg mL-1 of aqueous extract) and peroxide activity (92.1 % reduction at 200 µg mL-1 of ethanol and 93.1 % reduction at 200 µg mL-1 of aqueous extract). The antioxidant activity reported with ethanol (157.4 mmol Fe2+/g) and aqueous extract (129.8 mmol Fe2+/g) was also studied by analyzing with FRAP reduction. The obtained findings confirmed the high antioxidant activity of plant leaf extract and its application for pharmacological activity.

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Phytochemical Profiling Using Lc-Q-Tof-MS Analysis and in Vitro Antioxidant Activity of a Rare Salt-Secreting Mangrove Aegialitis Rotundifolia Roxb. Leaves Extract

Cited by 3 publications

References 33 publications

Seasonal Variations in Antioxidant Capacities and Phenolic Contents of Tea Leaf Extracts

Seasonal Variations in Antioxidant Capacities and Phenolic Contents of Tea Leaf Extracts

Metabolic profiling of bioactive compounds from different medicinal plants: An overview

Screening of Aegialitis Rotundifolia Roxb. Leaves for Their Antioxidant Potential, Polyphenol Content and Flavonoid Content

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