In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

Hu, Xiaoke; Cook, Sean; Wang, Peng; Hwang, Huey‐Min

doi:10.1016/j.scitotenv.2009.01.033

Cited by 280 publications

(145 citation statements)

References 14 publications

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“…Sin embargo, ya que las NP tienen largas áreas de superficie, alta reactividad química, volumen de poro interno y elevada permeabilidad celular, son propensas a inducir muchos efectos tóxicos (2,16) . El efecto de las NP depende de factores tales como tamaño, concentración, forma de exposición, la línea celular empleada, el tipo del organismo modelo, la relación cantidad: volumen y el tipo de NP; la literatura es diversa para cada uno de estos factores y en ella se exponen resultados variados (2,5,8,18) .…”

Section: Discussionunclassified

“…La reducción del MTT fue más alta en las NP de ZnO, seguidos por las NP de SiO 2 y NP de TiO 2 , respectivamente. Estos resultados son semejantes a los obtenidos por Zhan et al (9) y Hu et al (16) . La relación directa de la dosis de NP con el porcentaje de la reducción del MTT, también es observada para las alteraciones morfológicas y la muerte celular (9) .…”

Section: Discussionunclassified

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Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Zarria-Romero

Osorio

Pino

et al. 2017

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Evaluar el efecto de las nanopartículas de ZnO, TiO 2 y SiO 2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito -macrófago (GM-CSF) en Mus musculus. Materiales y métodos. Se extrajo médula ósea roja de cinco roedores (Balb/c) para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO 2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas deTiO 2 , 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados. Las nanopartículas de ZnO y SiO 2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO 2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente), con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO 2 (91%). Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones. Las nanopartículas de ZnO, SiO 2 y TiO 2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón. Palabras clave: Nanopartículas; Interleucinas; Expresión génica; Mus musculus (fuente: DeCS BIREME). EFFECT OF THE INDUSTRIAL NANOPARTICLES TiO 2 , SiO 2 and ZnO ON CELL VIABILITY AND GENE EXPRESSION IN RED BONE MARROW OF Mus musculus ABSTRACT Objectives.To evaluate the effect of ZnO, TiO 2 and SiO 2 nanoparticles on cell viability and expression of the interleukin 7, interleukin 3, and granulocyte-macrophage colony stimulating factor (GM-CSF) genes in Mus musculus. Materials and methods. Red bone marrow was extracted from five Balb/c mice for the analysis of cell viability using the MTT test. The mice were divided into two groups of five each: one group was inoculated intraperitoneally with 0.5, 1.0, 2.5, 5.0, and 10 mg/kg of ZnO and SiO 2 nanoparticles, respectively, and the other group was inoculated with 5.0, 10.0, 15.0, 20.0, and 25 mg/kg of TiO 2 nanoparticles, respectively. Thirty hours later, RNA was extracted from the red bone marrow of the mice in both groups for gene expression analysis using quantitative PCR and RT-PCR. Results. ZnO and SiO 2 nanoparticles reduced cell viability in a dose-dependent manner by 37% and 26%, respectively, starting at a dose of 1 mg/kg. TiO 2 nanoparticles at 5 mg/kg and 10 mg/kg reduced the gene expression of interleukins 7 and 3 by 55.3% and 70.2%, respectively, and SiO 2 nanoparticles caused the greatest decrease (91%) in the expression of GM-CSF. ZnO nanoparticles reduced the expression of GM-CSF starting at doses of 20 mg/kg and 25 mg/kg. Conclusions: ZnO, SiO 2 and TiO 2 nanoparticles affect cell viability and gene expression in the mo...

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Section: Discussionunclassified

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Zarria-Romero

Osorio

Pino

et al. 2017

Rev Peru Med Exp Salud Publica

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“…However, it is well known that plants have developed biochemical defense strategies against intracellular ROS accumulation, where the production and the elimination of ROS are in balance under unstressed conditions [27]. It has been proposed for the toxic mechanism of action of many nanomaterials to induce a strong oxidative stress altering the balance between oxidant and antioxidant cellular processes [28,29]. In the present study, when the L. gibba plant was exposed to AgNPs, the increase of intracellular ROS concentration was inducing an oxidative stress, therefore disturbing cellular biochemical functions.…”

Section: Discussionmentioning

confidence: 99%

Silver nanoparticle toxicity effect on growth and cellular viability of the aquatic plant Lemna gibba

Oukarroum

Barhoumi

Pirastru

et al. 2013

Enviro Toxic and Chemistry

188

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Abstract-The toxicity effect of silver nanoparticles (AgNPs) on growth and cellular viability was investigated on the aquatic plant Lemna gibba exposed over 7 d to 0, 0.01, 0.1, 1, and 10 mg/L of AgNPs. Growth inhibition was demonstrated by a significant decrease of frond numbers dependent on AgNP concentration. Under these conditions, reduction in plant cellular viability was detected for 0.1, 1, and 10 mg/L of AgNPs within 7 d of AgNPs treatment. This effect was highly correlated with the production of intracellular reactive oxygen species (ROS). A significant increase of intracellular ROS formation was triggered by 1 and 10 mg/L of AgNP exposure. The induced oxidative stress was related to Ag accumulation within L. gibba plant cells and with the increasing concentration of AgNP exposure in the medium. The authors' results clearly suggested that AgNP suspension represented a potential source of toxicity for L. gibba plant cells. Due to the low release capacity of free soluble Ag from AgNP dissolution in the medium, it is most likely that the intracellular uptake of Ag was directly from AgNPs, triggering cellular oxidative stress that may be due to the release of free Ag inside plant cells. Therefore, the present study demonstrated that AgNP accumulation in an aquatic environment may represent a potential source of toxicity and a risk for the viability of duckweeds. Environ. Toxicol. Chem. 2013;32:902-907. # 2013 SETAC

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“…As more and more nanomaterials are introduced into our daily life, there is a serious lack of information concerning their human health and environmental implications [2]. The integration of nanotechnology and biology provides the opportunity for the development of new materials in the nanometer size range with many potential applications in biological sciences and clinical medicine [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Ada

Türk²,

Oğuztüzün³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 μg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 μg/mL concentration for 16 h, but high in 400-500 μg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 μg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.

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In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

Cited by 280 publications

References 14 publications

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Silver nanoparticle toxicity effect on growth and cellular viability of the aquatic plant Lemna gibba

Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

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