In vitro evaluation of immunogenic properties of active dressings

Abstract

The aim of this study was in vitro evaluation of the level of the immune response in relation to wound dressings composed of alginate, calcium carboxymethylcellulose, and dibutyrylochitin and determination of the direction of response, which will make referring next to the results of in vivo phase possible. The subject of the experiments was to examine the commercially available, biodegradable alginate dressing, commercially available but not biodegradable dressing constructed from the sodium carboxymethylcellulose, and synthesized in house biodegradable dressing constructed of the dibutyrylchitin. To determine the direction of the immune response, the degree of secretion of pro-inflammatory interleukin (IL-1, IL-6) and antiinflammatory (IL-10) interleukin from murine fibroblasts having contact with the tested dressings (ELISA enzyme linked immunosorbent assay), was tested.

Alginate-Based Composite and Its Biomedical Applications

“…Free-dried porous sheets and fibrous dressing that followed are formed. Alginate dressings are characterized by absorbing wound fluid, physiologically moist environment, maintain the optimal pH, and reduced bacterial infections, that improve the physicochemical stability [36,37].…”

Blood Coagulation Activities of Cotton–Alginate–Copper Composites

“…It is believed that alginate-based wound dressings provide an appropriate drainage of wound exudates and prevent the entry of pathogenic bacteria [9][10][11][12][13]. Alginate's high content of water allows it to ensure the proper moist environment [5], which in turn helps to keep the optimal pH [18,24]. This is of the utmost importance because it was proven that a wet environment is considerably more effective in terms of wound healing [2].…”

“…Moreover, some studies suggest that alginate promotes the growth of the new epidermis [5]. Thus, alginate-based materials are considered to accelerate the process of hemostasis and wound healing [15,18,21,23,24].…”