In vitro Evaluation Of Odontogenic Differentiation Potential Of Adipose Tissue-Derived Stem Cells In Presence And Absence Of Nano-Hydroxyapatite Crystals.

Odontogenic Differentiation Potential Of Adipose Tissue Stem Cells In The Presence Of Odontogenic Stimuli Compared To Dental Pulp Stem Cells [An In-vitro Study].

“…Both types of cells displayed an increase in odontogenic differentiation genes expression of DMP, DSSP, and ALP, indicating the ability of ODM in the presence of NHA to differentiate DPSCs and ATSCs into odontogenic-like cells. And this agrees with several studies which examined the effect of NHA on odontogenic differentiation potential either on DPSCs [23,20] or ATSCs [24] .…”

“…However, many authors have studied the ability of ATSCs to differentiate into mineralizing cells for hard tissue regeneration such as bone [3] and dentine [24,32,33] . Some of these studies have revealed that ATSCs display an odontogenic potential in vitro [34] [35] , but the inductive stimulus required for odontogenic differentiation induction of ATSCs has not been fully explained.…”

“…In our previous study, it was proved that the addition of nanohydroxyapatite (NHA) to the odontogenic differentiation medium (ODM) significantly increased the odontogenic differentiation potential of ATSCs more than culturing them in growth media or ODM alone [24] . So, in the current study, the odontogenic differentiation potential of ATSCs has been compared to that of DPSCs in vitro in the presence of ODM and NH as a proven successful odontogenic differentiation stimulus for both types of cells.…”

“…For odontogenic induction, the cells were incubated in an odontogenic differentiation medium (ODM) [20,23] mixed with NHA crystals [23,24,25] The cells were placed in 6-well plate at a density of 5x10 4 cell/well with ODM [which is GM supplemented with 15% FBS, 10 mM -glycerophosphate, 0.2 mM/L ascorbate-2phosphate, and 100 nM dexamethasone (Sigma-Aldrich, USA)] supplemented with 10 g/mL NHA crystals for 14 days for better odontogenic induction. The cytotoxicity test of this concentration of NHA was previously tested on BMSCs [26] and ATSCs [24] . DPSCs and ADCs were seeded into 6-well plates at a density of 5x104/well.…”

“…In our previous work we investigated the odontogenic differentiation potential of ATSCs in presence of 3 different types of media. [24]. So, the current study aimed to compare between the odontogenic differentiation potential of adipose tissue stem cells (ATSCs) and dental pulp stem cells (DPSCs) in presence of odontogenic differentiation medium (ODM) and nano-hydroxy apatite crystals (NHA)…”