In vitro evaluation of osteoblastic cell adhesion on machined osseointegrated implants

Abstract

At present the major consideration in planning an implant design is to seek biocompatible surfaces that promote a favorable response from both cells and host tissues. Different treatments of implant surfaces may modulate the adhesion, proliferation and phenotypic expression of osteoblastic cells. For this reason, the aim of the present study was to evaluate the biocompatibility of an implant surface, observing adhesion, cell morphology and proliferation of osteoblast-like cells cultivated on a commercially available titanium dental implant (Titamax Liso  , Neodent, Curitiba, PR, Brazil). The implant samples were immersed into an osteoblast-like cell (Osteo-1) suspension for a period of 24, 48 and 72 hours. After seeding the cells, the samples were prepared for analyses through scanning electron microscopy. Based on the surface analysis, the osteoblastic cells adhered to the machined surface after 24 hours in culture. In 48 hours, the cells spread over the implant surface, and after 72 hours a proliferation of cells with large and flat bodies was observed over the machined implant surface. These results demonstrate that the machined titanium surface studied is biocompatible since it allowed adhesion and proliferation of the osteoblast-like cells, in addition to preserving cell integrity and the morphologic characteristics of cells during the studied period.

Evaluation of bone ingrowth into porous titanium implant: histomorphometric analysis in rabbits

“…4 Therefore, many efforts to modify the titanium implant surface for improved tissue response have been reported. 19,20,21 The advantage of porous materials is their ability to provide biological anchorage for surrounding bone tissues 18,22 and greater contact area at the implant-bone interface. 4,9 Additionally, the bone-filled porous structure is a composite, an important area of transition between the titanium core and bone.…”

Surface Characteristics and Cell Adhesion: A Comparative Study of Four Commercial Dental Implants

“…Currently no cell adhesion study compares implants from different companies. One study to compare the cell adhesion on the actual implant surface examined implants from only one company, which was not internationally recognized . To the best of our knowledge, our study is the first cell adhesion study to directly compare commonly used implants from different companies.…”

“…Currently, there is a lack of information regarding the direct comparison of cell culture or in vivo comparison among those dental implant systems. One recent publication compared the cell adhesion on the actual implant surface; however, the study only selected implants from one company, and the products are not internationally recognized …”

In Vitro Evaluation of Osteoblast-Like Cell Adhesion and Proliferation on Titanium Oxide Film Formed by Sand-Blasted Titanium Anode Treatment Process

“…In addition, rough surfaces enhance osteoblastic phenotype differentiation and the capacity of osteoblasts to synthesize bone matrix (1).…”