In vitro evaluation of Radachlorin® sensitizer for photodynamic therapy

Streptococcus mutans is the main etiological agent for dental caries. Recently, photodynamic therapy (PDT) has been introduced as a new modality in bacterial decontamination.Objective This in vitro study was carried out to evaluate the susceptibility of Streptococcus mutans to antibacterial PDT using two different photosensitizers and light sources. Material and Methods Standard suspensions of S. mutans were exposed to laser light at 662 nm and Radachlorin® or LED 630 nm in combination with Toluidine blue O (TBO). Radiation-only groups, photosensitizer alone, and groups with no treatment were used as controls. Bacterial suspension from each treatment was subcultured onto the surface of Mueller-Hinton agar plates and bacterial growth was assessed. The results were analyzed by analysis of variance and Tukey test (p<0.05). Results PDT with TBO and Radachlorin® significantly reduced S. mutans viability, whereas no difference was observed between two groups of PDT. In the groups treated just with the photosensitizer or irradiated alone, no significant reduction of S. mutans colonies was observed. ConclusionS. mutans colonies were susceptible to either 662 nm laser or LED light in the presence of Radachlorin® and TBO respectively with no priority.

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“…It is a chlorophyll a derivative, including mainly sodium chlorin e6 5 . Research on PDT with Radachlorin ® is limited.…”

Section: Discussionmentioning

confidence: 99%

“…Radachlorin ® was the other photosensitizer which was used in combination with laser 662 nm. It is a chlorophyll a derivative, including mainly sodium chlorin e6 5 . Research on PDT with Radachlorin ® is limited.…”

Section: Discussionmentioning

confidence: 99%

The susceptibility of Streptococcus mutans to antibacterial photodynamic therapy: a comparison of two different photosensitizers and light sources

et al. 2014

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“…Previous studies showed that aPDT mediated by this hydrosoluble chlorine was highly effective against Streptococcus mutans 21,22 . Photophysical characteristics of hydrosoluble chlorine —including water solubility, high quantum yield of singlet oxygen (75%), low toxicity in the dark, intensive absorption band in the middle‐red part of the spectrum, quicker kinetics in comparison with Ce6 alone, and high phototoxicity make it a unique agent as a photosensitizer for aPDT 22‐27 …”

Section: Methodsmentioning

confidence: 99%

Effect of Hydrosoluble Chlorine–Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature‐Induced Periodontitis in Dogs

Sorkhdini

Moslemi

Jamshidi

et al. 2013

Journal of Periodontology

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“…23 The porphyrins and their metallicderivatives act efficiently as sensitizing agents and they have presented phototoxic activity against different pathogens, such as bacteria, fungi, viruses and parasites. [24][25][26][27] Different porphyrinic photosensitizers have reported anti-leishmanicidal activity against Leishmania tarentolae in promastigote stage (ethyl and diethyl carbaporphyrin), against amastigotes of L. panamensis, and promastigotes of L. major and L. braziliensis (β-substituted porphyrinic systems). [28][29][30][31][32] Improved pharmacological responses have been found when incorporating metals in the macrocycle, as reported by Gomes et al when evaluating the activity against L. amazonensis against derivatives metalated with Bi (III) and Sb (IV) (IC50 of 93.8 μM and 52.4 μM respective).…”

Section: Introductionmentioning

confidence: 99%

Photophysical study and in vitro approach against Leishmania panamensis of dicloro-5,10,15,20-tetrakis(4-bromophenyl)porphyrinato Sn(IV)

et al. 2021

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Background: Photodynamic therapy activity against different biological systems has been reported for porphyrins. Porphyrin modifications through peripheral groups and/or by metal insertion inside the ring are main alternatives for the improvement of its photo-physical properties. In this study, we synthesized and characterized 5,10,15,20-tetrakis(4-bromophenyl)porphyrin and the dicloro-5,10,15,20-tetrakis(4-bromophenyl)porphyrinato Sn(IV). Methods: Metal-free porphyrin was synthesized using the Alder method, while the Sn(IV)-porphyrin complex was prepared by combining metal-free porphyrin with stannous chloride in DMF; the reaction yields were 47% and 64% respectively. Metal-free porphyrin was characterized by UV-Vis, FT-IR, ESI-mass spectrometry and 13C-NMR. Additionally, the Sn(IV) -porphyrin complex was characterized using UV-Vis and FT-IR. Cyclic voltammetry tests in four different solvents. The fluorescence quantum yield (Φf) was measured using fluorescein as a standard, the singlet oxygen quantum yield (ΦD) was estimated using the standard 5,10,15,20-(tetraphenyl)porphyrin (H2TPP) and the quencher of singlet oxygen 1,3-diphenylisobenzofuran (DPBF). Results: UV-Vis assay showed typical Q and Soret bands for porphyrin and its metallo-porphyrin complex. Compounds showed photoluminescence at the visible range of electromagnetic spectrum. The inclusion of the metal in the porphyrin core changed the Φf from 0.15 to 0.05 and the ΦD increased from 0.55 to 0.59. Finally, the effect of the compounds on the viability of L. panamensis was evaluated by means of the MTT test. The results showed that both compounds decreased the viability of the parasite; this inhibitory activity was greater under light irradiation; the porphyrin compound had IC50 of 16.5 μM and the Sn(IV)-porphyrin complex had IC50 of 19.2 μM. Conclusion: The compounds were synthesized efficiently, their characterization was carried out by different spectroscopy techniques and their own signals were evidenced for both structures, both compounds decreased the cell viability of L. panamensis.

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In vitro evaluation of Radachlorin® sensitizer for photodynamic therapy

Cited by 40 publications

References 23 publications

The susceptibility of Streptococcus mutans to antibacterial photodynamic therapy: a comparison of two different photosensitizers and light sources

The susceptibility of Streptococcus mutans to antibacterial photodynamic therapy: a comparison of two different photosensitizers and light sources

Effect of Hydrosoluble Chlorine–Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature‐Induced Periodontitis in Dogs

Photophysical study and in vitro approach against Leishmania panamensis of dicloro-5,10,15,20-tetrakis(4-bromophenyl)porphyrinato Sn(IV)

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