In vitro evaluation of selected Indian spices for α-amylase and α-glucosidase inhibitory activities and their spice-drug interactions

Mehrotra, Nupur; Jadhav, Kaustubh; Rawalgaonkar, Swati; Khan, Sara Anees; Parekh, B. R.

doi:10.21276/ap.2019.8.2.5

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…Acarbose is a standard antidiabetic drug that slows down the procedure of disintegration of polysaccharides and prevents hyperglycemia. Although it is well known that phytoconstituents in E. cardamomum have an antidiabetic effect [20], it was suggested by Sadowska-Bartosz et al [21] that various polyphenols and flavonoids attach to proteins and block glycation reactions.…”

Section: Discussionmentioning

confidence: 99%

Green Nanosuspensions of Elettaria Cardamomum Seeds – Comparative Data Analysis for Enhanced Bioactivities

Nadeem

Kayani

Hussain

2022

PJBB

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In order to conclude the efficiency of a plant for a specific ailment, its phytochemicals are evaluated using different scientific procedures. In this study, the aim was to evaluate the bioactive compounds and biological activities of Elettaria cardamomum seeds extract and nanosuspension. Nanosuspensions were formed using nano-precipitation method. Among bioactivities, antioxidant property was measured through total flavonoid content (TFC), total phenolic content (TPC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) activity. Antidiabetic activity was evaluated through alpha-amylase inhibition and antiglycation assays. Cytotoxicity and antibiofilm activity was determined using the hemolytic assay and micro-titer dish assay respectively. For functional groups and structural characterization FTIR was performed. In TPC and TFC the results showed 358.89 and 126.27 mg gallic acid equivalent to 100gram dry weight, 137.78 and 65.145 mg catechin equivalent to 100gram dry weight for nanosuspensions and extract respectively. Whereas the DPPH inhibition was 31.3% and 32.1% for nanosuspensions and extract respectively. Nanosuspensions and extract showed growth resistance against E. coli i.e. 68.12% and 64.09% respectively. Cardamom seeds nanosuspension and extract showed 55.77% and 55.15% antidiabetic property through glycation inhibition. According to alpha amylase inhibition analysis 51.67% and 33.67% inhibiting property of nanosuspension and extract was observed. Hemolytic assay gave 18% and 32% hemolysis by using nanosuspension and extract. The nanosuspension that showed existence of sulfoxides, fluoro compounds, amines, phenols, alkaloids and alcohols as determined by FTIR. In order to do this seeds were obtained and ethanol extract was formulated. This study showed that nanosuspensions and extract prepared from Elettaria cardamomum exhibited enhanced bioactivities and further research is essential to determine remarkable characteristics and pharmacological potentials of these unique seeds.

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Section: Discussionmentioning

confidence: 99%

Green Nanosuspensions of Elettaria Cardamomum Seeds – Comparative Data Analysis for Enhanced Bioactivities

Nadeem

Kayani

Hussain

2022

PJBB

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“…In vitro potency of plant's extract and fractions provides a platform for further research in the molecular label. Synergetic effect of active phytochemicals such as alkaloids, tannins, phenols, saponins, terpenoids, flavonoids, steroids, and sterols contribute to the plant's medicinal properties [24]. Due to the adverse effect of synthetic drugs the efficacy of plant-based food profiles with potent inhibitors is in demand.…”

Section: Discussionmentioning

confidence: 99%

α-Glucosidase and α-Amylase Inhibition Activities of Sarcococca coriacea Hook. And Sarcococca wallichii Staph. of Nepalese Origin

Baral

Shrestha

Adhikari

2022

J. Nepal Chem. Soc.

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Diabetes mellitus is being severe health problem globally with an increasing number of patients every day. Due to the lack of effective and non-toxic medicine to cure diabetes, plants that are used in ethnomedicine may be a good source for antidiabetic drug discovery. Plants of the Sarcococca genus are medicinally important and are used by local people for managing many diseases including diabetes. In the course of our continuous search of antidiabetic plants and pure compounds, in vitro α-glucosidase, and α-amylase inhibition activity along with the antioxidant activity of methanolic extract of S. coriacea leaf ( Sc-A), S. coriacea stem (Sc-B), and dichloromethane fraction of methanolic extract of Sarcococca wallichi (Sw-D) were carried out. The research revealed dicholoromethane fraction of S. wallichii (Sw-D) with good inhibition of α- amylase enzyme (IC50= 53.79 ± 2.50), whereas Sc-B inhibits α-glucosidase (20.97±2.37) effectively. Similarly, Sc-A showed significant antioxidant activity with IC50=24.56±3.3. The total phenolic content on Sc-A and Sc-B was calculated as 151.35±4.42 mg GAE/g and 86.22±1.59 mg GAE/g whereas the total flavonoid content on Sc-A and Sc-B was found to be 21.61±4.88 mg QE/g and 24.09±4.02 mg QE/g respectively. Similarly, total phenolic and total flavonoid content on Sw-D were found to be 85.26±3.16 mg GAE/g and 21.57±1.26 mg QE/g. To the best of our knowledge, this is the first report of α-glucosidase and α-amylase inhibition activity in these plants. This research work has scientifically supported the use of these plants to manage diabetes by local people and has explored new plants for antidiabetic drug discovery research.

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In vitro evaluation of selected Indian spices for α-amylase and α-glucosidase inhibitory activities and their spice-drug interactions

Cited by 2 publications

References 34 publications

Green Nanosuspensions of Elettaria Cardamomum Seeds – Comparative Data Analysis for Enhanced Bioactivities

Green Nanosuspensions of Elettaria Cardamomum Seeds – Comparative Data Analysis for Enhanced Bioactivities

α-Glucosidase and α-Amylase Inhibition Activities of Sarcococca coriacea Hook. And Sarcococca wallichii Staph. of Nepalese Origin

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